万古长空,一朝风月——关于7的整除性

我记得那天夜里，我从禅寺走向我在寺里的宿处时，只见月光下的树木和我自己皆澄彻透明。——铃木大拙《禅天禅地》[编按]     

Rare earth effect of yttrium on formation and property of Cr_2O_3 oxide film formed on Co-Cr binary alloy

The isothermal oxidizing kinetics of Co-40Cr alloy and its yttrium ion-implanted samples were studied at 1000 ℃ in air by thermal-gravity analysis (TGA). Scanning electronic microscopy (SEM) was used to examine the Cr2O3 oxide film's morphology after oxidation. Secondary ion mass spectrum (SIMS) method was used to examine the binding energy change of chromium caused by Y-doping and its influence on formation of Cr2O3 film. Acoustic emission (AE) method was used in situ to monitor the cracking and spalling of oxide films formed on both samples during oxidizing and subsequent air-cooling stages. Theoretical model simulating the film fracture process was proposed to analyze the acoustic emission spectrum both on time domain and on AE-event number domain. It is found that yttrium ion-implantation can remarkably reduce the isothermal oxidizing rate of Co-40Cr and improve the anti-cracking and anti-spalling properties of Cr2O3 oxide film. Reasons for the improvement are mainly that the implanted yttrium can reduce the grain size of Cr2O3 oxide, increase the high temperature plasticity of oxide film, and remarkably reduce the number and size of Cr2O3/Co-40Cr interfacial defects.     

Microbial starch-binding domains are superior to granule-bound starch synthase I for anchoring luciferase to potato starch granules

Microbial starch-binding domains (SBD) and granule-bound starch synthase I (GBSSI) are proteins which are accumulated in potato starch granules. The efficiency of SBD and GBSSI for targeting active luciferase reporter proteins to granules during starch biosynthesis was compared. GBSSI or SBD sequences were fused to the N- or C-terminus of the luciferase (LUC) gene, via an artificial Pro-Thr encoding linker sequence. The genes were introduced into an amylose-free (amf) potato mutant. It appeared that SBD was superior to GBSSI as a targeting sequence, mainly because the luciferase retained higher activity in the SBD-containing fusion proteins than in the GBSSI-containing ones.     

植物环肽

首先简要地介绍了植物环肽的定义、研究历史、结构分类、生物活性与生物功能等,最后以石竹科类型环肽和环蛋白为例详细介绍其检测方法、结构研究方法、化学合成或生物合成等.     

基于EXCEL和MATLAB的矩形薄片热传导计算与仿真研究

对二维导热问题进行了离散化研究,推导出了节点差分方程,以矩形薄片为例,用MS.Excel对节点温度进行了计算,用MATLAB对计算结果进行了仿真,得到了薄片的温度分布仿真图.     

Mutations in genes encoding melanosomal proteins cause pigmentary glaucoma in DBA/2J mice.

Pigmentary glaucoma is a significant cause of human blindness. Abnormally liberated iris pigment and cell debris enter the ocular drainage structures, leading to increased intraocular pressure (IOP) and glaucoma. DBA/2J (D2) mice develop a form of pigmentary glaucoma involving iris pigment dispersion (IPD) and iris stromal atrophy (ISA). Using high-resolution mapping techniques, sequencing and functional genetic tests, we show that IPD and ISA result from mutations in related genes encoding melanosomal proteins. IPD is caused by a premature stop codon mutation in the Gpnmb (GpnmbR150X) gene, as proved by the occurrence of IPD only in D2 mice that are homozygous with respect to GpnmbR150X; otherwise, similar D2 mice that are not homozygous for GpnmbR150X do not develop IPD. ISA is caused by the recessive Tyrp1b mutant allele and rescued by the transgenic introduction of wildtype Tyrp1. We hypothesize that IPD and ISA alter melanosomes, allowing toxic intermediates of pigment production to leak from melanosomes, causing iris disease and subsequent pigmentary glaucoma. This is supported by the rescue of IPD and ISA in D2 eyes with substantially decreased pigment production. These data indicate that pigment production and mutant melanosomal protein genes may contribute to human pigmentary glaucoma. The fact that hypopigmentation profoundly alleviates the D2 disease indicates that therapeutic strategies designed to decrease pigment production may be beneficial in human pigmentary glaucoma.     

利用计算机求解Hanoi塔问题

本文对著名的Hanoi塔问题利用递归关系设计出算法，在计算机上用C 编码实现，并对它的算法复杂度进行了分析。     

黄羊滩沙地的形成及其对北京沙尘暴天气的影响

对黄羊滩沙地进行物质成分分析、粒度分析和扫描电镜观察,结果表明:黄羊滩沙地的物质具有二源性特征,分别来源于黄羊滩洪积台地洪积物和洋河冲积物.根据沙地发育条件和历史过程,认为黄羊滩沙地不会有大的扩展.以粒度数据为基础,估算出黄羊滩沙地沙丘φ值大于4的沙尘年平均输出量为7.98×102 t*a-1,φ值大于7的沙尘年平均输出量为3.23×102 t*a-1,占年沙尘输出量的40.5%.北京沙尘暴沙尘组分以φ值大于6.8的为主,黄羊滩沙地对北京沙尘暴天气的沙尘有一定的贡献量.     

广西产眼镜王蛇毒α-神经毒素基因的分子克隆及序列分析

为获得眼镜王蛇（Ophiophagus hannah，简称Oh）蛇毒α－神经毒素（α-NT）的基因序列，依据眼镜蛇科不同毒蛇种类来源的α－NT基因有较高的同源性，设计1对上下游引物，为克服引物带来模糊扩增，在蛋白编码部分再设计1对上下游特异引物，用Nacleospin RNA Kit法从3条活眼镜王蛇毒腺中提取mRNA，以3′端引物合成的cDNA作为模板进行PCR扩增反应，测定产物的核苷酸序列，得到全长474bp的眼镜王蛇cDNA基因核苷酸序列。该核苷酸序列的信号肽与眼镜蛇树属Pseudonnaja textilis(Pt)、海蛇Laticauda semifasciata(Ls)100%同源，与眼镜蛇南洋亚种Naja sputatrix (Ns)、银环蛇（Bungarus multicinctus)(Bm)96.8%同源；蛋白密码部分有83．3％与Ns、79．2％与Pt、76．4％与Ls、74．1％与Bm同源。信号肽后紧接着的72个氨基酸有90．3％与已发现的眼镜王蛇毒长链α-NT Toxin a同源，大约有73．6％与Toxin b、69．7％与Oh-4、66．7％与Oh-5、56．9％与Oh-6A和6B同源，并与α－银环蛇毒素54．2％同源。说明新发现的眼镜王蛇cDNA是一条长链α-NT基因。     

The genesis and environmental significance of red and black sedimentary interlayers in coral reef of well Nanyong 2

The results of paleogeomagnetics and geochemistry of the coral reef in well Nanyong 2 of Nansha Islands showed that the bottom of the black sedimentary interlayer corresponds to the conversion boundary line between Brunhes Postive Polarity and Matuyama Reversed Polarity (B/M) and the cold/warm (19/20) climatic conversion bounds on δ¹⁸O curve, 0.78 Ma ago; and the red sedimentary interlayer corresponds to the ReunionⅠpolarity excursion (reversion) of the geomagnetic field, 2.01—2.04 Ma ago. Comparing with the normal light-coloured coral reef rock, the magnetic susceptibility (c), residual magnetization intensity (Mr) and the content of MnO and Fe₂O₃ of the black sedimentary layer appeared obviously positive abnormity. The magnetic susceptibility (c), residual magnetization intensity (Mr) and the content of Fe₂O₃ of the red sedimentary layer also appeared positive abnormity. Combining with the analyzing results of paleontology, we hold that this pair of special and typical sedimentary interlayers was relative to the sudden change of paleoclimate, i.e. the global climatic change and its incidental polarity reversal of the geomagnetic field directly affected the living environment of the paleontological species as well as the dispersion and enrichment of some chemical elements, especially the elements sensitive to redox such as Fe and Mn. For example, the elements Fe and Mn concentrated in the glacial period would be largely oxidized and diluted when the climate warmed up suddenly, and the originally oxidized high valence Fe would be condensed again when the climate cooled suddenly. This is possibly one of the important reasons of appearing and disappearing of the red and black sedimentary events of coral reef in well Nanyong 2.