天佛指山松茸资源属性及其可持续发展

充分阐述了天佛指山松茸资源的自然属性、可保护属性和保护区的科学管理基础及条件，指出这些是天佛指山松茸资源可持续发展雄厚的软、硬件基础，而争取到国家级自然保护区的管理力度和条件，加快科学技术应用和成果转化，形成现代化的松茸资源监控管理体系和松茸资源信息管理系统，将强有力地促进天佛指山乃至整个长白山区松茸资源的可持续发展。     

混合系统:回顾与展望

本文首先对混合系统的概念及研究背景进行了介绍,然后综述了混合系统的分析与设计,重点讨论了分析切换系统稳定性的统一李雅普诺夫函数法、多李雅普诺夫函数法,介绍了混合系统的最优控制、混合系统的验证与仿真.对混合系统未来的研究趋势进行了展望,并进一步指出混合系统研究的重要意义.     

一种改进的三电平逆变器空间电压矢量PWM控制技术

根据三电平逆变器空间电压矢量PWM控制原理,针对过调制问题,提出了一种改进的空间电压矢量PWM控制技术,并推导了三电平逆变器空间电压矢量在各个过调制区域内的等效作用时间算法.仿真实验结果表明该方法在过调制时能够保证线性调制关系,有效抑制谐波,提高电压利用率.     

A complete sequence and comparative analysis of a SARS-associated virus(Isolate BJ01)

Severe Acute Respiratory Syndrome (SARS) is a newly identified infectious disease[1—5]. The global outbreak of SARS has been threatening the health of people worldwide and has killed 353 people and infected more than 5462 in 27 countries, as reported by WHO on April 29, 2003 (http://www.who.int/csr/sarscountry/en). Although it has been recognized that a variant of virus from the family of coronavirus might be the candidate pathogen of SARS[1—5], its identity as the unique pathogen sti…     

大型GIS海量数据分布式组织与管理

海量空间数据是制约GIS发展的一个瓶颈，是大型GIS的根本特征．从应用的角度给出了大型GIS和海量数据的定义，提出了造成海量数据的原因，指出解决问题的关键之一是建立分布式数据库，实现海量数据的分布式存储和管理，这又使得GIS数据共享的含义发生了变化．利用Java、JDBC和CORBA技术，设计了两种不同的解决方案：(1)基于元数据的异质数据获取；(2)基于FDBS的异质数据库一体化．目前第一种方案较为可行，第二种方案还有一些技术问题需要解决，后以前为基础，实现前，对后会有极大的帮助，并且由于设计的构件化。可以随着技术的不断发展和成熟，逐步地向前添加有关构件，实现大型GIS分布式组织与管理的渐进发展．     

试题库智能组卷算法的设计与实现

智能计算机辅助教学中一个重要问题是试题库的智能组卷。通过对组卷约束条件的描述，建立了该问题的数学模型，给出了解决此问题所涉及的主要数据结构及组卷搜索算法。实验结果表明，该方法具有较好的性能和实用性。     

体育教学与德育渗透的思考与实践

体育教学中渗透着丰富的德育内容，对于学生人格的培养、世界观的形成具有积极的作用。在体育教学中，根据学生的特点，有意识地渗透德育内容，进行生动活泼的思想道德教育，取得了良好效果。     

Design and application of 60mer oligonucleotide microarray in SARS coronavirus detection

In April 2003, a novel coronavirus[1,2] which was associated with cases of Severe Acute Respiratory Syn-drome (SARS) was first isolated and sequenced in Canada. The genome of SARS coronavirus (SARS-CoV) is 29727[3] nucleotides in length and has 11 known open reading frames (ORFs). Although the genome organiza-tion of this virus is similar to that of other coronaviruses, phylogenetic analyses and sequence alignment show that SARS-CoV is not closely related to any of the previously ch…     

南海海底特征、资源区位与疆界断续线

根据南海海底的成因与地形特征 ,并通过建立和分析南海海底数字地形模型 ,将南海的海底分为中央的深海盆地、两侧的阶梯状大陆坡、以及周边亚洲大陆延伸的大陆架 3个地貌单元 .再据 194 7年“南海诸岛位置图”精确计算出二战后依据波茨坦公告为国际认可的断续线疆界的经纬度坐标 ,阐述了中国对南海主权的历史延续性 .最后 ,论述了南海区位与资源的优势 ,论证了其海域具体位置与巨大的经济政治战略意义 .     

Inhibitory effect of the adenovirus type 5 E1A protein expressed in the yeast system of the human tumor cell growth

Adenovirus 5 type E1A as a tumor suppressor gene can inhibit tumor growth and enhance the censitivity of chemotherapy and radiotherapy.E1A have the ability to integrate into the host genome,resulting in long-time expres-sion that induces Rb gene inactivation and animal cells im-mortalization.This prompted us to select the E1A protein for treatment of cancer in order to overcome the limitations of E1A gene therapy.Thus,we firstly comstructed E1A eu-caryotic expression vector (pPIC9/E1A),transformated the pichia pastoris yeast cells(GS115) and screened the high-expressing recombinant strains.The positive yeast strains were cultured in the shake flask,and induced for 3d.The crude E1A protein was purified using two steps of col-umu chromatography on HiTrap Q and HiTrap SP.The pu-rified E1A protein was identified by SDS-PAGE and Western blot.E1A protein was mostly located at cellular unclear when Cheriot delivered E1A protein into cells.The analysis in vitro indicated that the E1A protein arrested LN686 cell cycle at G2/M phase,and significantly inhibited the growth of LN686 tumor cells.The current studies firstly provided an experimental basis to further develop E1A protein for tumor treatment.