全文获取类型
收费全文 | 30077篇 |
免费 | 556篇 |
国内免费 | 1025篇 |
专业分类
系统科学 | 1080篇 |
丛书文集 | 706篇 |
教育与普及 | 269篇 |
理论与方法论 | 104篇 |
现状及发展 | 6299篇 |
研究方法 | 782篇 |
综合类 | 21897篇 |
自然研究 | 521篇 |
出版年
2022年 | 312篇 |
2021年 | 353篇 |
2020年 | 309篇 |
2018年 | 237篇 |
2017年 | 275篇 |
2016年 | 345篇 |
2015年 | 593篇 |
2014年 | 830篇 |
2013年 | 958篇 |
2012年 | 1237篇 |
2011年 | 1858篇 |
2010年 | 1175篇 |
2009年 | 1211篇 |
2008年 | 1486篇 |
2007年 | 1600篇 |
2006年 | 1367篇 |
2005年 | 1229篇 |
2004年 | 890篇 |
2003年 | 647篇 |
2002年 | 746篇 |
2001年 | 868篇 |
2000年 | 857篇 |
1999年 | 643篇 |
1998年 | 229篇 |
1992年 | 366篇 |
1991年 | 306篇 |
1990年 | 292篇 |
1989年 | 263篇 |
1988年 | 245篇 |
1987年 | 236篇 |
1986年 | 206篇 |
1985年 | 252篇 |
1984年 | 236篇 |
1980年 | 200篇 |
1979年 | 445篇 |
1978年 | 340篇 |
1977年 | 367篇 |
1976年 | 264篇 |
1975年 | 322篇 |
1974年 | 446篇 |
1973年 | 379篇 |
1972年 | 360篇 |
1971年 | 421篇 |
1970年 | 484篇 |
1969年 | 425篇 |
1968年 | 407篇 |
1967年 | 442篇 |
1966年 | 384篇 |
1965年 | 301篇 |
1958年 | 231篇 |
排序方式: 共有10000条查询结果,搜索用时 31 毫秒
51.
52.
Rogaeva E Meng Y Lee JH Gu Y Kawarai T Zou F Katayama T Baldwin CT Cheng R Hasegawa H Chen F Shibata N Lunetta KL Pardossi-Piquard R Bohm C Wakutani Y Cupples LA Cuenco KT Green RC Pinessi L Rainero I Sorbi S Bruni A Duara R Friedland RP Inzelberg R Hampe W Bujo H Song YQ Andersen OM Willnow TE Graff-Radford N Petersen RC Dickson D Der SD Fraser PE Schmitt-Ulms G Younkin S Mayeux R Farrer LA St George-Hyslop P 《Nature genetics》2007,39(2):168-177
The recycling of the amyloid precursor protein (APP) from the cell surface via the endocytic pathways plays a key role in the generation of amyloid beta peptide (Abeta) in Alzheimer disease. We report here that inherited variants in the SORL1 neuronal sorting receptor are associated with late-onset Alzheimer disease. These variants, which occur in at least two different clusters of intronic sequences within the SORL1 gene (also known as LR11 or SORLA) may regulate tissue-specific expression of SORL1. We also show that SORL1 directs trafficking of APP into recycling pathways and that when SORL1 is underexpressed, APP is sorted into Abeta-generating compartments. These data suggest that inherited or acquired changes in SORL1 expression or function are mechanistically involved in causing Alzheimer disease. 相似文献
53.
1 Results Pyrimidinones (PMs) are a class of important heterocycles which have been well documented throughout the literature due to their biological importance. They exhibit a wide range of pharmaceutical and therapeutic properties[1].A rapid and efficient one-pot method for the synthesis of 4,6-diarylpyrimidin-2(1H)- ones and related heterocycles is described.The condensation of acetophenone derivatives,aldehydes and urea in the presence of sulfamic acid was employed to synthesize a variety of pyrimid... 相似文献
54.
物理化学实验多媒体课件的设计与应用 总被引:3,自引:0,他引:3
论述了物理化学实验课件经过多年的制作实践是如何从内容到方法逐步完善的设计理念 .使完成的物理化学实验课件在教学中得到广泛的应用 ,并收到传统教学所不能获得的教学效果 ,同时也得到了学生和专家以及同行的肯定 . 相似文献
55.
省级旅游市场供给环境及其评价方法研究--以山东省为例 总被引:1,自引:0,他引:1
余洁 《山东师范大学学报(自然科学版)》2002,17(4):51-54
旅游市场供给的内容十分丰富,其质量和效益的高低可通过对旅游市场的供给环境分析加以确定。本文在对旅游市场供给环境做简要分析的基础上,主要采用层次分析法对山东省旅游市场供给环境做出综合评价,为供给方充分利用和认识市场环境,发挥优势,寻找市场机会提供参考依据。 相似文献
56.
57.
吕玉芳 《济源职业技术学院学报》2002,1(1):48-51
中国共产党始终代表中国先进生产力的发展要求的论断,是站在历史唯物主义的角度,揭示出我们党兴旺发达的根本动力. 相似文献
58.
将快速傅里叶变换技术和数字滤波器设计技术应用于铝电解过程控制信号的滤波与噪声解析模型中,在160kA中间点式下料预焙槽上的试验结果表明了模型的有效性。 相似文献
59.
Nuclear localization and signalling activity of phosphoinositidase C beta in Swiss 3T3 cells. 总被引:14,自引:0,他引:14
The hydrolysis of phosphatidylinositol 4,5-bisphosphate (PtdInsP2) is a widespread receptor-coupled signalling system at the plasma membrane of most eukaryotic cells. The existence of an entirely separate nuclear phosphoinositide signalling system is suggested from evidence that purified nuclei synthesize PtdInsP2 and phosphatidylinositol 4-phosphate (PtdInsP) in vitro and that a transient decrease in the mass of these lipids occurs when Swiss 3T3 cells are cultured in the presence of insulin-like growth factor-1 (IGF-1). These IGF-1-dependent changes in inositol lipids coincide with an increase in nuclear diacyglycerol and precede translocation to the nucleus and activation of protein kinase C (refs 5, 6). Circumstantial evidence that links these changes with mitosis comes from the isolation of a 3T3 clone that expresses the type-1 IGF receptor and binds IGF-1 peptide but does not respond mitogenically or show transient mass changes in nuclear inositol lipids. A key question is how IGF-1 initiates the rapid breakdown of PtdInsP and PtdInsP2 in the nucleus. Here we present evidence that nuclei of 3T3 cells contain the beta-isozyme of phosphoinositidase C, whereas the gamma-isozyme is confined to the cytoplasm and that IGF-1 treatment stimulates exclusively the activity of nuclear phosphoinositidase C. 相似文献
60.
To validate procedures of rational drug design, it is important to develop computational methods that predict binding sites between a protein and a ligand molecule. Many small molecules have been tested using such programs, but examination of protein-protein and peptide-protein interactions has been sparse. We were able to test such applications once the structures of both the maltose-binding protein (MBP) and the ligand-binding domain of the aspartate receptor, which binds MBP, became available. Here we predict the binding site of MBP to its receptor using a 'binary docking' technique in which two MBP octapeptide sequences containing mutations that eliminate maltose chemotaxis are independently docked to the receptor. The peptides in the docked solutions superimpose on their original positions in the structure of MBP and allow the formation of an MBP-receptor complex. The consistency of the computational and biological results supports this approach for predicting protein-protein and peptide-protein interactions. 相似文献