Effect of thrombin on the apoptosis of hippocampal neurons in vitro

Hippocampal neurons were treated by thrombin and thrombin receptor activating peptides (TRAP). Cell survival rate was decreased in a dose-dependent manner by MTT assay. The numbers of apoptotic cell and apoptotic rate of hippocampal neurons treated by different concentrations of thrombin were increased in a dose-dependent manner by terminal deoxynucleotidyl transferase (TdT) mediated dUTP-biotin nick end-labeling (TUNEL) method and Flow Cytometry. When the concentration of thrombin is 40 U/mL, TUNEL positive cells and apoptotic rate of hippocampal neurons reached peak value, were 27.3±4.0 and (29.333±4.633)%, respectively. Immunocytochemistry assay show that Bcl-2 protein expression was down-regulated and Bax protein expression was up-regulated with the concentration of thrombin increased. TRAP can mimic the effect of thrombin to induce apoptosis on hippocampal neurons. These data demonstrated that thrombin induced hippocampal neuron apoptosis in a dose-dependent manner through activating protease-activated protein-1 (PAR-1). The change in expression of Bcl-2 and Bax was related with the effect of high concentration thrombin induced apoptosis on hippocampal neurons. Foundation item: Supported by the Natural Science Foundation of Hainan Province (N30215) Biography: YANG Wen-qiong (1968-), female, Ph.D. candidate, research direction: cerebrovascular disease.     

一种新型双层PBG结构带阻滤波器

本文介绍了一种双层结构的基于村底打孔的PBG结构带阻滤波器，采用了周期结构与微带电路分离的设计。解决了传统的打孔结构的导带加工问题，并且有利于电路的加工和修改。最后给出了利用HFSS理论仿真和实际测量的结果，论证了这种结构的可行性     

Atomic-scale computer simulation for early precipitation process of Ni75AlxV25-x alloy with intermediate Al composition

The microscopic phase-field approach is applied to model the early precipitation process of Ni75AlxV25-x alloy. Without any prior assumptions, this model can be used to simulate the temporal evolution of arbitrary morphologies and microstructures on atomic scale. By simulating the atomic pictures, and calculating the order parameters and volume fraction of the θ (Ni3V) and γ'(Ni3Al) ordered phases, we study Ni75AlxV25-x alloys with Al composition of 0.05, 0. 053 and 0. 055 (atom fraction). Our calculated results show that,for these alloys, θ and γ' phases precipitate at the same time; with the increase of Al content, the amount of γ' phase increases and that of θ phase decreases; the precipitation characteristic of γ' phase transforms from Non-Classical Nucleation and Growth (NCNG) to Congruent Ordering Spinodal Decomposition (CO SD) gradually; otherwise, the precipitation characteristic of θ phase transforms from Congruent Ordering Spinodal Decomposition (CO SD) to Non-Classical Nucleation and Growth (NCNG) mechanism gradually. Both θ and γ' phases have undergone the transition process of mixture precipitation mechanism characterized by both NCNG and CO SD mechanisms. No incontinuous transition of precipitation mechanism has been found.     

A template of rat brain based on fMRI T*2 imaging

The development of functional magnetic resonance imaging (fMRI) technology has made it possible to carry out functional brain imaging experiments in small animals. Usually, group data is required to form the assessment of population, which can not only increase the sensitivity of the overall experiment, but also allow the generalization of the conclusion to the whole population. In order to average the signals of functional brain images from different subjects, it is necessary to put all the mapping images into the same standard space (template image). However, up to now, most animal brain templates remain unavailable and it must be done by ourselves. In this study, a template image based on the brains of eight male Wistar rats is obtained, and it is successfully used in our present Alzheimer disease (AD)-like rat model studies as template for spatially normalizing images to the same stereotaxical space. The fMRI results processed with statistical parametric mapping (SPM99) software are in agreement with the results from immunohistochemical experiment, which proves that this method is universally applicable to the pathologic models of other small animals and to human brain lesion studies.     

发展循环经济推进新型工业化

循环经济是一种新的经济发展模式,发展循环经济是推进新型工业化的最优路径选择,现阶段发展循环经济推进新型工业化面临许多障碍,发展循环经济必须有新的对策.     

西部民族地区在大开发中所面临的生态环境问题与思考——以内蒙古自治区鄂托克旗为例

实施西部大开发战略，加快中西部地区发展，是党中央面向新世纪做出的战略决策。位于内蒙古自治区西部的鄂托克旗，是一个典型的蒙古族聚居地区，自然资源丰富、生态环境脆弱，具有我国西部民族地区的典型特征。鄂托克旗所面临的生态环境问题在我国西部民族地区具有较强的代表性，其形成原因及解决方法对于我国西部大开发中的西部民族地区具有普遍意义。     

人工神经网络预测高炉铁水硅含量

提出了BP神经网络预测高炉铁水硅含量的几种网络优化方法,指出了神经网络预测硅含量的可行性与局限性.     

三峡水利枢纽二期工程科技和管理创新

峡工程规模巨大、技术复杂，针对重大技术难题，汇集全国科技精华，充分发挥专家的作用，展开科技攻关，并借鉴国外先进经验，科学决策。2003年6月1日水库开始蓄水，6月10日水位到达135 m高程，6月16日双线五级船闸试通航成功，左岸电站首批水轮发电机组从7月份开始投产，到2003年底已有6台机组正式并网发电。三峡工程已经顺利地实现了蓄水、通航、发电的二期工程建设目标。在枢纽总布置及大坝工程、水电站工程、双线连续五级船闸、特大型水轮发电机组、导截流及围堰工程和特大型工程管理技术研究及实践方面，取得了一系列重大     

线性人脸对象类模型的匹配提升技术

针对真实感人脸模型匹配的细节控制和稳健创建问题，提出了线性人脸对象类模型的匹配提升技术。基于非统一抽样(NUS)的动态高斯金字塔分析(DGPA)方法，结合不等概率抽样和整群抽样策略，自适应地动态调整每级高斯金字塔图像的抽样分布，利用最优化算法由粗到精的计算全局近似最优解，获得精确的模型匹配。动态调节整群区域边界并利用再抽样率调节抽样密度，可以有效控制人像模型的细节表达效果，提高模型创建的稳健性。随机梯度下降的线性相关性扰动(CD-SGD)和学习率自适应(ALR)技术，提高了模型匹配的准确性和收敛速度。以M     

基于最大熵原理的复合形法及其在边坡稳定分析中的应用

将最大熵原理应用于基本复合形法的优化计算中，旨在提高算法的寻优成功率。具体步骤为：选取复形中熵最大的顶点为寻优点；在复形其余各点的加权中心点与寻优点的连线上，搜索比寻优点改善的新顶点；并替换掉寻优点构成新的复形；如此迭代直至复形中的信息被完全利用。采用基本复合形法和基于最大熵原理的复合形法对两个复杂土坡的最小安全系数进行了搜索，结果表明基于最大熵原理的复合形法的全局搜索能力有了明显改善。