Mechanism of the interaction between Au nanoparticles and polymerase in nanoparticle PCR

Nanoparticle PCR is a novel method to optimize DNA amplification. It performs well in improving specificity, enhancing sensitivity and speed. Several mechanisms were proposed in previous studies: one was based on the interaction between gold nanoparticles (AuNPs) and DNA while the other was attributed to the heat transfer property of AuNPs. In this paper, we propose that the interaction between AuNPs and DNA polymerase can significantly influence PCR. First, the addition of DNA polymerase can eliminate the inhibitory effects of excess AuNPs. Second, the addition of AuNPs will increase yield of the desired PCR product and make the optimum concentration of DNA polymerase move to higher value. Third, while excess polymerase might inhibit amplification efficiency, AuNPs can reverse this process and the yield of PCR amplification. Based on these results we propose a possible mechanism that AuNPs might modulate the activity of polymerase and improve PCR amplification.     

温度、湿度的变化对云冈石窟保存的影响

分析了云冈石窟1962年2窟泉水温度和当时气温的监测数据及2003年全年气象站和窟内6个监测点的温度、湿度数据:总结了窟区和各种类型洞窟内温度、湿度的变化情况及岩石内部温度的变化情况;计算了窟外和各种类型洞窟内可能发生冻融的条件和时间;揭示了不同季节的水分在岩石和大气间运移情况;讨论了温度、湿度对云冈石窟的影响机理.认为修建窟檐对石雕的保存有利,但也存在湿度增大的问题.因此,建议在温度、湿度变化较大的洞窟,应修建窟檐,同时要考虑适当的排湿措施;没有窟檐的洞窟,在湿度过大的地方,也应采取必要的排湿措施.     

有机化学教学改革尝试

在高等教育的改革和发展过程中,教学思想的转变、教学方法和教学内容等诸方面的改革是重要内容。重点对有机化学教学中教学方法和教学内容的改革进行了探讨。     

固定义齿CAD/CAM中标准牙建模方法

从口腔解剖生理学和CAD技术两个角度出发,分析标准牙模型的特征分布规律,提出用牙体表面形态特征和几何构造特征构建标准牙曲面模型.根据牙尖、牙窝等形态特征在牙体表面的分布特点,以及与之关联的龈缘线、近牙合线等几何构造特征,从标准牙点云模型中识别各个特征点.再利用这些特征点构建UV曲线,最终获得能够满足口腔临床修复要求的标准牙曲面模型,为各种修复体的设计提供很好的依据.     

贵阳市紫林庵路口通行能力分析及改进措施

作者以目前贵阳市紫林庵信号环形交叉口为例,通过对此路口高峰时段交通量调查及相关数据采集,对该交叉口的通行能力进行分析和计算.再假设在其它相关数据不变的基础上,分析和计算将现在的信号环形交叉口改为信号平面直交口的通行能力.将两者进行比较和评价,得出信号平面直交口的设计通行能力比信号环形交叉口的设计通行能力更大,更能缓解该地区高峰时段交通拥堵状况.由此得到将现有的环形交叉口改为平面直交口的方案是合理可行的.     

山西省镁工业污染防治现状与对策研究

介绍了国内外镁工业的发展与污染现状，针对山西省镁工业发展与污染的现状以及污染防治中存在的问题，提出了污染防治的对策措施。     

有机碳源环境下的厌氧氨氧化批式实验

通过厌氧氨氧化批式实验,研究了在有机碳源环境下COD/NH4 -N比、pH值以及NO2--N浓度对厌氧氨氧化反应的影响.结果表明:在有机碳源环境下,厌氧氨氧化作用和反硝化作用可以同时存在;适宜的COD/NH4 -N比值范围在0~1.57之间;适宜的pH值范围应该在6.02~8.50之间,最适pH值为8.00;为了得到较好的脱碳和脱氮效果,在初始COD值为300mg/L时,初始NO2--N浓度不宜超过500mg/L,否则会抑制厌氧氨氧化反应和反硝化反应的进行.     

A new estimator of covariance matrix via partial Iwasawa coordinates

This paper is concerned with the problem of improving the estimator of covariance matrix under Stein’s loss. By the partial Iwasawa coordinates of covariance matrix, the corresponding risk can be split into three parts. One can use the information in the weighted matrix of weighted quadratic loss to improve one part of risk. However, this paper indirectly takes advantage of the information in the sample mean and reuses Iwasawa coordinates to improve the rest of risk. It is worth mentioning that the process above can be repeated. Finally, a Monte Carlo simulation study is carried out to verify the theoretical results.     

Ruin probabilities with pairwise quasi-asymptotically independent and dominatedly-varying tailed claims

This paper considers the nonstandard renewal risk model in which a part of surplus is invested into a Black-Scholes market whose price process is modelled by a geometric Brownian motion, claim sizes form a sequence of not necessarily identically distributed and pairwise quasi-asymptotically independent random variables with dominatedly-varying tails.The authors obtain a weakly asymptotic formula for the finite-time and infinite-time ruin probabilities.In particular,if the claims are identically distributed and consistently-varying tailed,then an asymptotic formula is presented.     

跨校区形势下提高新校区教学质量的研究

教学质量是高等院校的立校之本,跨校区教学给新校区教学质量的保证和提高带来了很多前所未有的困难。针对大连民族学院金石滩校区当前存在的一些问题进行研究,并提出一些建设性意见和相应的解决方法,对新校区办学水平和教学质量的进一步提高具有实际意义。