双层钢箱截面组合索塔力学性能试验

设计制作了5个具有开孔板连接件和5个具有焊钉连接件的双层钢箱截面钢-混凝土组合索塔模型试件,根据索塔实际受力特性进行轴压和恒定轴力下的往复弯曲荷载试验.试验展示了使用两类连接件的双层钢箱组合索塔在不同荷载作用下的破坏形态和极限能力,结果表明:开孔板和焊钉均能约束受压钢板的屈曲波长,连接件的类型对试件破坏的具体形态有一定影响;轴压比是影响试件力学性能的主要因素,所有试件都根据其大小分为受压破坏和受拉破坏两类;无轴力和过高的轴力都会明显降低构件在往复荷载下的力学性能.最后,将试验结果与按照基于刚塑性原理的规范计算得到的承载能力进行比较.     

Unresponsiveness of colon cancer to BRAF(V600E) inhibition through feedback activation of EGFR

Inhibition of the BRAF(V600E) oncoprotein by the small-molecule drug PLX4032 (vemurafenib) is highly effective in the treatment of melanoma. However, colon cancer patients harbouring the same BRAF(V600E) oncogenic lesion have poor prognosis and show only a very limited response to this drug. To investigate the cause of the limited therapeutic effect of PLX4032 in BRAF(V600E) mutant colon tumours, here we performed an RNA-interference-based genetic screen in human cells to search for kinases whose knockdown synergizes with BRAF(V600E) inhibition. We report that blockade of the epidermal growth factor receptor (EGFR) shows strong synergy with BRAF(V600E) inhibition. We find in multiple BRAF(V600E) mutant colon cancers that inhibition of EGFR by the antibody drug cetuximab or the small-molecule drugs gefitinib or erlotinib is strongly synergistic with BRAF(V600E) inhibition, both in vitro and in vivo. Mechanistically, we find that BRAF(V600E) inhibition causes a rapid feedback activation of EGFR, which supports continued proliferation in the presence of BRAF(V600E) inhibition. Melanoma cells express low levels of EGFR and are therefore not subject to this feedback activation. Consistent with this, we find that ectopic expression of EGFR in melanoma cells is sufficient to cause resistance to PLX4032. Our data suggest that BRAF(V600E) mutant colon cancers (approximately 8-10% of all colon cancers), for which there are currently no targeted treatment options available, might benefit from combination therapy consisting of BRAF and EGFR inhibitors.     

含风电电力系统随机生产模拟的改进算法

针对传统随机生产模拟忽略负荷的时序特性而难以考虑机组启停、备用、调峰等相关动态费用的问题,将转移频率分析纳入随机生产模拟框架中,形成了改进等效电量频率法.该方法通过等效负荷频率曲线(ELFC)的卷积考虑机组启停,将生产成本分析的范畴拓宽到动态费用的计算.根据所提算法和含风电的EPRI-36算例,比较了风电并网前后系统可靠性指标、燃料成本、环境成本和动态费用等的变化,并研究了风电装机规模对动态费用率的响.结果表明,相对于动态费用的增加,风电对系统可靠性与经济性的改善是主要的,但动态费用率随着风电规模的扩大而提高.     

Recent advances in hydrogen storage technologies based on nanoporous carbon materials

Hydrogen is a promising energy carrier that can potentially facilitate a transition from fossil fuels to sustainable energy sources without producing harmful by-products. Prior to realizing a hydrogen economy, however, viable hydrogen storage materials must be developed. Physical adsorption in porous solids provides an opportunity for hydrogen storage under low-stringency conditions. Physically adsorbed hydrogen molecules are weakly bound to a surface and, hence, are easily released. Among the various surface candidates, porous carbons appear to provide efficient hydrogen storage, with the advantages that porous carbon is relatively low-cost to produce and is easily prepared. In this review, we summarize the preparation methods, pore characteristics, and hydrogen storage capacities of representative nanoporous carbons, including activated carbons, zeolite-templated carbon, and carbide-derived carbon. We focus particularly on a series of nanoporous carbons developed recently: metal–organic framework-derived carbons, which exhibit promising properties for use in hydrogen storage applications.     

脑-机接口中小波和小波包方差的特征比较

针对两种不同意识任务的脑-机接口设计,提出了以方差作为特征的方法和以分类速率作为评价标准之一的新方法.首先深入研究了小波理论,分析了小波包分解中存在的频带交错现象,然后以小波系数和小波包系数的方差作为特征,对C3,C4导联脑电信号分别进行两种特征的提取,最后采用线性支持向量机作为分类器进行分类.结果表明,两种特征对应的最大分类正确率均达到了86.43%,对应时间分别为4.32和4.31 s.因此,以小波方差和小波包方差作为特征是完全可取的;分类速率的提出能同时反映分类正确率和分类时间,为大脑意识任务分类提供了新思路.     

重庆抗战诗歌在期刊媒介场域中的版面争夺

抗战时期,重庆成为政治、文化中心,为文学的繁荣提供了有利的经济条件。重庆抗战诗歌的繁荣与重庆众多文学期刊的创办、复刊有着一种共生关系,这种关系的形成促成了期刊媒介场域的生成。而期刊媒介场域内部也发生着某种"权力"争斗,从而推动着重庆抗战诗歌的发展。     

起爆方式对整体式MEFP战斗部参数的影响

为研究不同起爆方式对整体式多爆炸成形弹丸MEFP战斗部参数的影响,通过LS-DYNA仿真软件,对3种不同起爆方式下战斗部形成多个弹丸过程进行数值仿真,并结合爆轰波作用理论,对弹丸成形形状和速度进行理论分析。结果表明：当采用点起爆与环形起爆时,弹丸束发散角和毁伤面积较大,但周边弹丸速度较低,形状不规则,气动性较差;当采用平面起爆时,弹丸束发散角和毁伤面积最小但弹丸速度较大,气动性较好;相对于点起爆、环形起爆方式,采用平面起爆方式时炸药有效利用率最高,形成弹丸的气动性和对目标侵彻效果最好。     

SKL自主创新能力评价指标设置的问卷分析

作为基础研究的"国家队",国家重点实验室自主创新能力的强弱是关键。对国家重点实验室自主创新能力评价指标的设置问题进行了相关专家的问卷调查,收回有效问卷108份。问卷分析结果表明,该套评价指标体系能够基本正确反映国家重点实验室自主创新能力,并为国家重点实验室进一步提高自主创新能力提供了科学指引。     

DLL3蛋白在人小细胞肺癌细胞中的功能及其机制

为探讨DLL3(human notch ligand delta-like 3)过表达对人小细胞肺癌细胞的影响及可能的作用机制,通过PCR方法扩增人DLL3基因全长序列,并克隆至慢病毒表达载体Lenti-EFS-FLAG-puro而构建人DLL3基因过表达慢病毒表达质粒,酶切及测序鉴定质粒正确。通过慢病毒包装及感染,构建DLL3稳定过表达的人小细胞肺癌细胞株,并通过Western blot验证DLL3蛋白的表达。采用CCK-8法检测DLL3过表达对人小细胞肺癌细胞的增殖的影响。采用平板克隆实验检测DLL3过表达对人小细胞肺癌细胞的克隆形成的影响。Western blot检测DLL3过表达对细胞周期相关蛋白Cyclin D1,Cyclin D3的表达水平的影响。结果表明:人DLL3过表达慢病毒表达质粒构建成功; CCK-8实验显示DLL3过表达促进人小细胞肺癌细胞的增殖。平板克隆实验显示DLL3过表达提高人小细胞肺癌细胞的克隆形成能力。Western blot结果表明DLL3过表达增加细胞周期蛋白Cyclin D1,Cyclin D3的表达水平。可见DLL3过表达对人小细胞肺癌细胞的增殖具有促进作用。     

A voltage-gated proton-selective channel lacking the pore domain

Voltage changes across the cell membrane control the gating of many cation-selective ion channels. Conserved from bacteria to humans, the voltage-gated-ligand superfamily of ion channels are encoded as polypeptide chains of six transmembrane-spanning segments (S1-S6). S1-S4 functions as a self-contained voltage-sensing domain (VSD), in essence a positively charged lever that moves in response to voltage changes. The VSD 'ligand' transmits force via a linker to the S5-S6 pore domain 'receptor', thereby opening or closing the channel. The ascidian VSD protein Ci-VSP gates a phosphatase activity rather than a channel pore, indicating that VSDs function independently of ion channels. Here we describe a mammalian VSD protein (H(V)1) that lacks a discernible pore domain but is sufficient for expression of a voltage-sensitive proton-selective ion channel activity. H(v)1 currents are activated at depolarizing voltages, sensitive to the transmembrane pH gradient, H+-selective, and Zn2+-sensitive. Mutagenesis of H(v)1 identified three arginine residues in S4 that regulate channel gating and two histidine residues that are required for extracellular inhibition of H(v)1 by Zn2+. H(v)1 is expressed in immune tissues and manifests the characteristic properties of native proton conductances (G(vH+)). In phagocytic leukocytes, G(vH+) are required to support the oxidative burst that underlies microbial killing by the innate immune system. The data presented here identify H(v)1 as a long-sought voltage-gated H+ channel and establish H(v)1 as the founding member of a family of mammalian VSD proteins.