A rapid and accurate 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide colorimetric assay for quantification of bacteriocins with nisin as an example

The objective of this study is to propose a more accurate and faster MTT [3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide] colorimetric assay (MCA) for quantitative measurement of polypeptide bacteriocins in solutions with nisin as an example. After an initial incubation of nisin and indicator bacterium Micrococcus luteus NCIB 8166 in tubes, MTT was added for another incubation period. After that, nisin was quantified by estimating the number of viable bacteria based on measuring the amount of purple formazan produced by cleavage of yellow tetrazolium salt MTT. Then MCA was compared to a standard agar diffusion assay (ADA). The results suggested a high correlation coefficient (r ²=0.975±0.004) between optical density (OD) and the inhibitory effect of nisin on a bacterial strain Micrococcus luteus NCIB 8166 at a range of 0.125~32 IU/ml. The MCA described in this study was very quick. Quantification of nisin took only 7~8 h and the detection limit was at the level of 0.125 IU/ml when compared to 12 IU/ml and 24~28 h for ADA. The MCA provides an accurate and rapid method for quantification of nisin in solutions and is expected to be used for quantification of other antimicrobial substances.     

35t/h工业链条炉计算机控制系统的设计

介绍了35t/h工业链条炉计算机控制系统,论述了各控制系统的实现,阐述了系统软件的设计。     

二元多项式环中理想生成元个数的估计

对于两个变量的多项式环中的理想,我们给出了其有限生成基个数的一个最小上界的估计.并由例子说明此估计是最佳的.     

AMC垃圾渗滤液处理工艺

介绍了AMC垃圾渗滤液处理的工艺流程,对系统单元进行了功用分析,探讨了AMC系统的特点。     

对高校图书馆人力资源管理工作的几点认识

针对当前高校图书馆工作的实际,就图书馆组织结构、人员结构、工作组织方式、激励管理及培训等方面提出了一些思考。     

黄连素的十大药理功能

本文通过查阅大量文献,综合分析近年来关于黄连素药理作用研究情况,总结出黄连素十大药理功能,旨在理顺有关黄连素的研究思路,为更加合理的研究开发和使用黄连素提供理论支持。     

我国国内旅游需求影响因素分析及规模预测

近20年来我国国内旅游业发展之所以形成高潮,一方面由于我国实行改革开放的结果,而另一方面更在于近年我国居民可支配收入和闲暇时间的增多.2003年上半年,中国旅游业遭受SARS的沉重打击,2003年下半年国内旅游业经历了迅速恢复期,2004年已经表现出很强的增长势头.就影响我国国内旅游业发展的主要因素进行分析,并对未来五年我国国内旅游发展规模进行预测.     

太中银线吕梁段方案研究

文章以太中银线吕梁段方案为例,分析了影响线路方案的主要因素,强调了线路选线过程中的主要技术标准以及与地方规划协调的重要性。     

A theoretical model for mid-and low-latitude ionospheric electric fields in realistic geomagnetic fields

The geomagnetic fields, which play important roles in the ionospheric dynamo, can greatly affect the global distribution of ionospheric electric fields, currents and other ionospheric electrodynamics phenomena. In the study of ionospheric electrodynamics phenomena, such as the longitudinal variations of ionospheric electric fields, the non-dipolar component of the geomagnetic fields must be taken into account. In this paper, we deduce a theoretical electric field model for ionospheric dynamo at mid- and low-latitude which adopt a modified magnetic apex coordinates system. In the new electric field model, the geomagnetic fields can be calculated from either the IGRF model or the dipole field model, and the neutral winds and conductivities are calculated based on empirical models. Then the dynamo equation for the electric potential is finally solved in terms of the line-by-line iteration method, and the ionospheric electric fields and currents are derived from the calculated potential. Our model can reproduce the main features of the ionospheric electrodynamics processes, so it will be a useful tool for the investigation of the upper atmosphere and ionosphere.     

HACCP在石油行业野外施工食堂中应用的绩效分析

将HACCP这一世界公认的食品安全控制系统引入野外施工食堂卫生管理当中.方法采用文献检索,事故分析、现场调查和Delphi等研究方法,通过对野外施工食源性疾病危害分析、建立关键控制点,形成野外施工食堂管理HACCP管理规范,并组织实施.结果实施HACCP体系后,从业人员手、餐具、菜墩、抹布的抽查合格率有显著提高,食堂从业人员以及参加施工人员对饮食卫生以及食堂卫生管理知识的掌握显著提高.结论在野外施工食品卫生管理中引入HACCP管理体系,控制食源性疾病取得了良好的绩效.