Establishment and cryopreservation of liver, heart and muscle cell lines derived from the Chinese alligator （Alligator sinensis）

The Chinese alligator, Alligator sinensis, is a critically endangered species. A conservation project of gene resources for an endangered species first involves the preservation of organs, tissues, gametes, genomic DNA libraries and cell lines. The present study is the first to establish and cryopreserve cell lines of liver, heart and muscle tissues from the Chinese alligator. The study revealed that there was a large discrepancy in cell migration time in primary cultures among liver (11-12 d), heart (13-14 d) and muscle (17-18 d) tissue pieces. The differences in time in primary cell culture suggested that it was relatively easy to build visceral-derived cell lines for reptiles. Biological analysis showed that the population doubling time for thawed cells was approxi- mately 36 h. Karyotyping revealed that the frequency of Chinese alligator cells showing chromosome number as 2n=32 was 88.6%-93.4%. Chinese alligator cell lines established here provide a vital resource for research and are likely to be useful for protection of this rare and critically endangered species. Furthermore, the establishment of these methods may supply technical and theoretical support for preserving genetic resources at the cellular level for other reptile species.     

Experimental study on the explosive boiling in saturated liquid nitrogen

Nowadays,thedevelopmentandapplicationofsuperconductionisbecomingmoreandmoreimpor tantinmanyareas .Duringtheworkingprocessofsu perconductor,somefactors ,forinstance ,deforma tion ,friction ,andmagneticvortex ,canresultinin stantaneousandrandomheatimpulsion ,andmakeitover heatedtothecriticaltemperatureanddeviatefromthenormaloperationcondition .Undersomeex tremeconditions ,thesuperconductorcannotgobacktothenormalstateevenifremovingthetransientheat load ,thusverysevereandunexpectableconse quencema…     

Crystallization and preliminary X-ray diffraction analysis of FKBP12 complexed with a new neurotrophic ligand

Thetherapyofnerveinjuryandneurode generativediseasessuchasAlzheimer’sdiseaseandParkinson’sdiseaseisadifficultprojectforscientiststosurmount.Manydrugsandtherapeuticapproacheshavebeenusedtotreatthesediseases ,buttheresultisunsatisfactory[1] .Inrecent years ,neurotrophinsincludingnervegrowthfactor (NGF) ,neurotrophin3(NT3) ,neurotrophin 4 / 5 (NT4 / 5 )andbrain de rivedneurotrophicfactor (BDNF)werestudiedex tensivelyandfoundtoaffectthesurvival,growth ,and/ordifferentiationofneuronsandsever…     

Hausdorff centered measure of certain linear Cantor sets

Thenotionofpackingmeasure ,introducedinRefs.[1— 3],hasbeenusedincomparisonwithHausdorffmeasuretostudytheregularityandrectifi abilityofsetsintheplane[4 ] .TheHausdorffmeasureisdefinedusingeconomicalcoversofaset,whilethepackingmeasureisdefinedviapackingwithdisjointballscenteredinthegivenset (formoredetailsseeRefs .[5— 7]) .Asasymmetryofthepackingmea sure ,theHausdorffcenteredmeasure ,definedbythecoverswithballscenteredinthegivenset,wasintro ducedinRef .[4 ].RaymondandTricot[8] verifiedthatt…     

浅谈卫生间装修的施工要点

从材料选型、排砖布局等方面阐述了卫生间整体装修策划的主要内容,详细介绍了卫生间装修工序,提出了卫生间装修施工的注意事项。     

单圈连通图的边独立指数

依据图的边独立指数概念,探讨了单圈连通图的边独立指数的相关性质,得到了单圈连通图边独立指数的上、下界,并给出了单圈连通图边独立指数达到上、下界的极图     

城市资源管理研究综述

随着城市化进程的加快,城市中的有限资源如何得到合理并持续的利用,这个问题受到越来越多人的关注,本文试图在城市管理的基础上,并以资源的角度为新的出发点,提出一个城市资源管理的概念,对此相关文献作系统的综述。     

实施内部会计控制 加强工程财务管理

你是我慈祥的母亲啊 我亲爱的祖国 从远古的荒原上你一路风尘 肩上挑满了风霜的岁月 可你永远是那样的谦和仁爱 从容走过所有的屈辱和挫折     

基础教育改革下高校体育教育专业人才培养对策研究

通过选择部分高校的体育教育专业的教师和人才培养方案(教学计划)、部分中小学体育教师作为研究对象,运用文献资料法、问卷调查法、数理统计法等研究方法,对基础教育改革下的我国高校体育教育专业人才培养现状进行深入调查和客观分析,找出当前我国高校体育教育专业人才培养目标定位、培养内容,以及体育教育专业学生的教育思想、创新能力、综合素质不能很好地适应基础教育体育与健康课程改革的要求等现实问题,对高校体育专业人才培养的发展定位、目标体系、具体内容提出了相应的对策和建议     

183例小儿重度、特重度烧伤的临床治疗方法分析

目的研究小儿重度、特重度烧伤的临床救治.方法对我院2006年1月至2011年12月收治的183例重度、特重度小儿烧伤临床资料进行回顾总结及分析.结果183例患儿中治愈167例,治愈率为91.26%.结论早期及时有效的抗休克治疗是成功救治的重要保证,全身合理使用抗生素是防治烧伤感染的重要措施,积极预防烧伤并发症、维持气道通畅及合理创面外用药物、早期切削痂植皮封闭创面,是成功救治的重要环节