含二羟硼基二茂铁化合物的合成和表征

以二茂铁甲酸和3-氨基苯硼酸为原料,合成了[(3-二羟硼基苯基)氨酰基]二茂铁(1),通过IR和1H NMR进行了表征.利用循环伏安法(CV)和差分脉冲法(DPV)对其进行电化学性质的研究.     

二甲醚-氢气-空气混合气预混燃烧的实验研究

在定容燃烧弹中,研究了不同燃空当量比、掺氢比和初始压力下的二甲醚-氢气-空气预混合气的一系列层流燃烧特性参数,并且系统地分析了当量比、掺氢比和初始压力对燃烧的影响.结果表明:随着掺氢比的增大,火焰传播速率、层流燃烧速率、燃烧压力升高率和质量燃烧速率都明显增大,火焰发展期和燃烧持续期则随之缩短;当掺氢比较低时,随着当量比的升高,马克斯坦长度不断递减,即稀混合气的燃烧稳定性更高;当掺氢比较高时,随着当量比的升高,马克斯坦长度不断递增,即浓混合气的燃烧稳定性更高;最高燃烧压力随着初始压力的升高而升高,受掺氢比的影响相对较小.     

莱茵衣藻玻璃珠法快速转化检测体系的建立

为了建立在衣藻中快速简便研究外源基因功能的方法,采用玻璃珠转化法将pBI221质粒（35S,Amp,GUS）高效转化至细胞壁缺陷型衣藻品系CW-15（Arg-）,通过对转化条件及GUS表达检测条件摸索,发现35S启动子在CW-15中能有效启动GUS表达,且GUS最佳检测时间约为转化后24h,重组细胞先染色后制片更有利于GUS检测.     

基于Mobile Agent的移动GIS模型

针对移动GIS应用中无线互联网络的带宽限制、非对称、不稳定等问题,采用了移动智能体(Mobile Agent)分布式计算理论与方法,提出了一种基于Mobile Agent的移动GIS模型.该模型集成Mobile Agent、GPS、CDMA、TCP/IP、UDP等方法与技术,可以进行空间信息的移动计算、移动目标的实时监控、空间信息的实时采集与无线传输等,并且该模型从底层初步构建了Mobile Agent运行环境、移动GIS应用服务器、移动GIS客户端和服务器等模块.利用该模型在上海某区消防网格化调查与维护应用中进行了试验,结果表明所提出的模型与方法在无线互联网络环境下具有较好的灵活性和实用性.     

尾矿坝稳定性分析

为了尾矿库的安全生产,需对尾矿坝进行中期的系统稳定性评价,采用数值方法针对具体工程实例进行渗流稳定性、静力稳定性和动力稳定性计算,结果表明:浸润线没有从坝坡出溢,水力坡降不大,渗流稳定.安全系数满足规范要求,坝体是静力稳定的.从坝体动力反应及液化结果得出该坝是动力稳定的.同时提供了一条稳定性评价的完整路线,用数值模拟方式对尾矿坝的计算有一定参考价值和指导意义.     

宽严相济：基于司法能力与被动的分析

宽严相济的刑事政策是我们党和国家提出的新的刑事政策,是司法机关准确实施国家法律的重要指针。依法办案是宽严相济的刑事政策的题中之义,刑事司法必须在现行法律范目内“从宽”与“从严”,但宽严相济的刑事政策不能被筒单理解为依法办案。在刑事司法中,司法者应当明确适用空间、抓住关键环节和区别案件情节。只有这样才能实现宽严相济的刑事政策。     

Cell-specific ATP7A transport sustains copper-dependent tyrosinase activity in melanosomes

Copper is a cofactor for many cellular enzymes and transporters. It can be loaded onto secreted and endomembrane cuproproteins by translocation from the cytosol into membrane-bound organelles by ATP7A or ATP7B transporters, the genes for which are mutated in the copper imbalance syndromes Menkes disease and Wilson disease, respectively. Endomembrane cuproproteins are thought to incorporate copper stably on transit through the trans-Golgi network, in which ATP7A accumulates by dynamic cycling through early endocytic compartments. Here we show that the pigment-cell-specific cuproenzyme tyrosinase acquires copper only transiently and inefficiently within the trans-Golgi network of mouse melanocytes. To catalyse melanin synthesis, tyrosinase is subsequently reloaded with copper within specialized organelles called melanosomes. Copper is supplied to melanosomes by ATP7A, a cohort of which localizes to melanosomes in a biogenesis of lysosome-related organelles complex-1 (BLOC-1)-dependent manner. These results indicate that cell-type-specific localization of a metal transporter is required to sustain metallation of an endomembrane cuproenzyme, providing a mechanism for exquisite spatial control of metalloenzyme activity. Moreover, because BLOC-1 subunits are mutated in subtypes of the genetic disease Hermansky-Pudlak syndrome, these results also show that defects in copper transporter localization contribute to hypopigmentation, and hence perhaps other systemic defects, in Hermansky-Pudlak syndrome.     

A two-tiered mechanism for stabilization and immobilization of E-cadherin

Epithelial tissues maintain a robust architecture which is important for their barrier function, but they are also remodelled through the reorganization of cell-cell contacts. Tissue stability requires intercellular adhesion mediated by E-cadherin, in particular its trans-association in homophilic complexes supported by actin filaments through beta- and alpha-catenin. How alpha-catenin dynamic interactions between E-cadherin/beta-catenin and cortical actin control both stability and remodelling of adhesion is unclear. Here we focus on Drosophila homophilic E-cadherin complexes rather than total E-cadherin, including diffusing 'free' E-cadherin, because these complexes are a better proxy for adhesion. We find that E-cadherin complexes partition in very stable microdomains (that is, bona fide adhesive foci which are more stable than remodelling contacts). Furthermore, we find that stability and mobility of these microdomains depend on two actin populations: small, stable actin patches concentrate at homophilic E-cadherin clusters, whereas a rapidly turning over, contractile network constrains their lateral movement by a tethering mechanism. alpha-Catenin controls epithelial architecture mainly through regulation of the mobility of homophilic clusters and it is largely dispensable for their stability. Uncoupling stability and mobility of E-cadherin complexes suggests that stable epithelia may remodel through the regulated mobility of very stable adhesive foci.     

纳米晶体TiO2光催化降解1-萘酚的路径

浓度为50mg／L的1-萘酚废水在二次微波法制备的TiO2为催化剂、H2O2为氧化剂和紫外光组成的光催化氧化体系中常温下被彻底降解,CODcr和TOC去除率分别达到86．49％和89．36％通过对降解反应全过程进行定时多点GC／MS检测,发现了60多种中间产物,并对中间产物的产生、变化及其相对舍量与反应时间的关系进行分析、归纳．确定了1-萘酚降解的3条主要路径,全面准确地揭示了降解过程的全貌,对1-萘酚在上述氧化体系中的降解规律进行了实践和理论上的有益探索．     

二元机翼模型在随机参数激励下的首次穿越问题

为研究机翼在随机参数激励下的稳定性和可靠性,建立了在一个随机气流速度影响下的二元机翼随机动力学模型．首先,利用拟不可积Hamilton系统的随机平均法,将系统的广义能量表示为一维Ito扩散过程;其次,利用奇异性边界理论讨论了系统的全局随机稳定性;最后,为研究机翼振动能量和噪声强度对机翼工作安全性能的影响,求得了系统可靠性函数和首次穿越（系统损坏）时间概率密度函数．结果表明：系统可靠性函数在接近安全工作域边界时降低得很快,能量初值远离安全域边界可降低损坏概率密度峰值并将对应的时刻推后;噪声强度的增大也会引起系统可靠性下降加快和首次穿越概率密度函数峰值上升．