Larval instars of the ant Strumigenys solifontis Brown (Hymenoptera: Formicidae): the fallacy of size distribution

Larval instars of the myrmicine ant Strumigenys solifontis Brown were determined by measuring the head width under a stereomicroscope and examining the morphology, especially chaetotaxy, by light microscopy and scanning electron microscopy. This study found that the larval stage consisted of four instars, but the first and second instars were not distinguishable by their head widths. However, the four instars could be discriminated clearly by a combination of size measurement and observations on the chaetotaxy. Within a population, there are few larvae in the first instar because of its short period (only 1–2 days before moulting into the second instar) and the low oviposition rate of the queen. Therefore, the first-instar larva needs to be collected separately from other instars so that it is not missed.     

Studies on Alenophalera bronneomixia (Lepidoptera: Notodontidae)—a pest of the West African indigo plant,Lonchocarpus cyanescens (Papilionaceae)

Summary

This paper is concerned with the general biology of the notodontid moth, A. bronneomixia whose larvae are found to be serious defoliators of the West African indigo plant, Lonchocarpus cyanescens.

At temperatures fluctuating between 21°C and 29°C (mean 22·5°C) and humidities between 70 to 95 per cent eggs developed and hatched in a mean of 11·1 days from deposition, mean hatch being 93 per cent.

Under the said environmental conditions, the life cycle from egg to adult entailed six larval instars and a prepupal stage before the real pupa, all lasting 60 to 80 days. Larvae were strongly monophagous, fed communally and in nature pupated in soil/litter very close to the foot of their invaded food plant.

Adults were retiring and in the field deposited clusters of numerous eggs preferably on the abaxial surfaces of their food plants. In nature, this moth's population seemed strongly regulated by high egg and larval parasitization, larval predation and fungal attacks at the pupal stage.     

Observations on the developmental biology of Cicindela arenicola Rumpp (Coleoptera: Cicindelidae)

Cicindela arenicola Rumpp is being considered for threatened and endangered status by the U.S. Fish and Wildlife Service. Little is known about the developmental biology of this species. Adults of C. arenicola were active from April through late June and late August through early November, but generally they were not on the surface when temperatures were below 19 C or above 45 C, or when conditions were windy, cloudy, or rainy. Mating and egg-laying occurred only during the spring. Five size classes (1-,2-,3-,4-, and 5-mm diameters) of larval burrow openings were observed. Burrows of 5 mm had a mean depth of 42.23 ± 8.81 cm (1 S.D.). Larvae of C. arenicola were active from April through June and late September through mid-November. Developmental times and larval size were affected by food supplementation. Nearly all food-supplemented, 1-mm, first instars molted to larger second instars of3-mm diameter. Most nonsupplemented larvae spent several months as 2-mm second instars before growing to 3 mm in diameter. Food-supplemented larvae reached the third instar in approximately 13 months, while nonsupplemented larvae took slightly over two years to reach this stage. Development from egg to adult was estimated at two years for food-supplemented larvae and three and one-half to four years for nonsupplemented larvae. Cattle had a significant effect on larval mortality. A high percentage (76-80%) of larval C. arenicola that were stepped on by cattle never reopened their burrows. Only 14% of undisturbed larvae never reopened their burrows. Approximately 15% of 150 larvae marked in 1988 reopened burrows in 1989.     

Are there costs to extended larval transport in the Trinidadian stream frog,Mannophryne trinitatis (Dendrobatidae)?

Previous work has shown that male Mannophryne trinitatis (Dendrobatidae) carry their larvae on their backs for up to 4 days in search of a predator‐free pool in which to deposit them. The experiments reported here investigated whether costs to the larvae or to the adults limit transport duration. We simulated transport durations of 0, 4, 8, and 12 days for larvae, but found no deterioration in terms of ability to grow to metamorphosis; indeed, 12‐day larvae grew better than all the others. After 8 days of simulated transport, larvae had used up all their yolk reserves and begun to lose dry weight. Larvae on wet substrates gained wet weight and length but on drier substrates merely maintained weight, suggesting that dehydration could be a problem on the male's back. In a trial of locomotor performance (mean jump length; number of jumps to traverse a runway), females performed best with calling males not significantly different from transporting males, despite an average larval load equivalent to 15–20% of the frog's mass. Assessment of gut contents showed that females foraged more than males, but that transporting males foraged as much as did calling males. We found no differences between the three classes of adult frogs in fat body weight.     

Bionomics,behaviour and immature stages of Pelecotoma fennica (Paykull) (Coleoptera: Rhipiphoridae)

Ptilinus fuscus (Anobiidae) was confirmed as the host species of Pelecotoma fennica (Rhipiphoridae, Pelecotominae). Females of Pelecotoma oviposit into the wood infested by the host larvae. First-instar larvae are elongate, unsclerotized, very different from the triungulinid larvae known in other rhipiphorids. They search actively for the host larvae in the wood; no phoresy is involved in the life cycle. The first-instar larvae temporarily paralyse the host larva and enter its body, overwintering (some perhaps more than once) as an endoparasite. In the spring of the year of emergence, the endoparasite engorges enormously (without moulting) and develops a special sclerotized caudal structure which is then used for perforation of the host's integument. The larva undergoes a further four ectoparasitic instars. The fifth (i.e. fourth ectoparasitic) instar differs considerably from the preceding three, and is capable of boring through the wood to prepare the emergence gallery for the adult. Pupation occurs in the wood. The rate of parasitization may locally far exceed 50%. Superparasitization by the first-instar larvae is possible, but usually only 1 larva survives to the ectoparasitic stage. Larval morphology of Pelecotoma is described and illustrated. Additional data on bionomics and larval morphology are also presented for the genus Metoecus (Rhipiphorinae). Comparing the biology and larval morphology of Pelecotoma with other Rhipiphoridae, it is assumed that ancestral rhipiphorids may have been xylophilous Tenebrionoidea with predaceous or omnivorous larvae, and that the ‘triungulinid’ larvae and phoretic habits may not belong to the groundplan of the family Rhipiphoridae. The widespread opinion considering Rhipiphoridae closely related to the family Mordellidae is questioned.     

Pherbina mediterranea: immature stages,biology, phenology and distribution (Diptera: Sciomyzidae)

The egg, three larval instars, and puparium of Pherbina mediterranea are described. The duration of the various immature stages indicates that this specis is univoltine and spends the winter as quiescent larvae. The biology, phenology and geographical distribution are presented. The main features of the larvae are illustrated with scanning electron micrographs. A key to the adults of genus Pherbina is given.     

Growth ‘rules’ and the larvae of decapod crustaceans

Summary

Statements that have attempted to express the relationship between the sizes of successive arthropodan instars are examined with reference to published data on larval decapod crustaceans.

The conclusions reached are (a) that ‘Brooks's Law’, which simply states that the lengths of successive instars form an exponential series, generally holds good in decapod larvae, (b) that ‘Przibram's Rule’, stating that the weight increase between instars is always by some power of 2, is not supported by the observed length changes, and (c) that Gurney's suggestion that growth factors in decapod larvae do not normally exceed about 1·5 is not applicable to the order as a whole, but may be valid for some sub-groups within it.     

Contrasting modes of handling moss for feeding and case‐building by the caddisfly Scelotrichia willcairnsi (Insecta: Trichoptera)

Final‐instar larvae of the microcaddisfly, Scelotrichia willcairnsi sp. nov. (Trichoptera: Hydroptilidae) were discovered feeding on a moss, Platyhypnidium muelleri in north‐eastern Queensland. Not only do the larvae feed on the moss, but their cases are constructed from fragments of moss leaves. Microscopic examination of larval guts and cases showed a total contrast in the angle at which the moss leaves are cut for each purpose: for cases the moss leaves are cut longitudinally, in parallel with the length of the cells, whereas for feeding the leaves are cut perpendicular to the leaf margin, across the cells, presumably an adaptation that releases the cell contents for digestion. The new caddisfly species is described based on the adult males. Scelotrichia willcairnsi represents the first Australian record of the South‐east Asian‐New Guinean Stactobiini genus Scelotrichia.     

Drumming behavior and life history notes of a high-altitude Colorado population of the stonefly Isoperla petersoni Needham

Late instar nymphs and adults of a Colorado Hudsonian zone population of Isoperla petersoni Needham and Christenson were studied during the summer and early fall months of 1998, when stream temperature ranged from 3.3° C to 8.9° C. Early and middle instar nymphs were absent from July to October, and nymphs attained maximum size in mid-August during the 2nd week of adult field presence, suggesting a univoltine-slow life cycle. Nymphs were carnivorous and fed primarily on chironomid larvae. Adults were present from August to mid-October, with peak numbers of adult males and females occurring in mid-September and late September, respectively. Fecundity of field-collected females averaged 94.1 ± 45.15 eggs per female in September ( N = 81) and decreased to 85.2 ± 57.6 in October ( N = 12). Drumming duets were 2-way (male-female), and female answers followed male calls (nonoverlapped) or began before completion of the male call (overlapped). Male calls averaged 11.1 ± 2.09 beats and female answers averaged 6.2 ± 2.99, with mean intervals of 118 and 58 ms, respectively. The average number of female answer beats increased from 5.3 ± 2.56 when duets were nonoverlapped, to 6.6 ± 3.08 when overlapped.     

Bionomics of two aspen bark beetles, Trypophloeus populi and Procryphalus mucronatus (Coleoptera: Scolytidae)

Beetles of Trypophloeus populi attacked green bark of unhealthy aspen ( Populus tremuloides ) and hastened the death of the tree. Beetles of Procryphalus mucronatus favored dead bark and were of little significance in the death of the aspen. These two beetle species were distinguished from each other by their primary galleries, eggs, larvae, larval mines, and mating behavior. In T. populi, there were three larval instars and one to one and one - half generations per year; only larvae overwintered, and the eggs of the first generation were present by mid - July. In P. mucronatus, there were two larval instars and one and one - half to two generations per year; both larvae and adults overwintered, and the first eggs of the new season were present by late May.     

Development of epipods and gills in some pagurids and brachyurans

Sepedon trichrooscelis is a semiaquatic, multivoltine species whose larvae feed on snails of the genus Succinea. The duration of the various developmental periods and larval biology are presented. The egg, three larval instars, and puparium of Sepedon trichrooscelis are described and illustrated. The distribution of the cuticular sensorial receptors, the shape of the hydrofuge setae on the posterior disc, and the details of the egg are illustrated by scanning electron micrographs.     

The life cycle of Hydroperla crosbyi (Plecoptera: Perlodidae)

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Developmental stages of Caligus epidemicus Hewitt,a copepod parasite of tilapia cultured in brackish water

The larval development of Caligus epidemicus comprises ten stages: two nauplii, one copepodid, six chalimus, and one preadult (young adult) stages. The morphology of each of these ten instars is described based on material reared from eggs in the laboratory. Addition of fifth and sixth chalimus stages in the ontogeny of C. epidemicus is discussed in the light of the developmental changes in the leg armature. Some peculiarities about the structure of the frontal filament in various chalimus stages are also discussed.     

Natural history of an ant–plant–butterfly interaction in a Neotropical savanna

Non-myrmecophilous lepidopteran larvae using plants bearing ant attractants such as extrafloral nectaries are good models for studying morphological and behavioural mechanisms against ant predation. Udranomia spitzi (Hesperiidae) is a butterfly whose larvae feed on leaves of Ouratea spectabilis (Ochnaceae), a plant with extrafloral nectaries. We described the early stages of U. spitzi, and used field observations and experiments to investigate the defensive strategies of caterpillars against predatory ants. Larvae pass through five instars and pupation occurs inside larval leaf shelters. Ant-exclusion experiments revealed that the presence of ants did not affect significantly caterpillar survival. Predation experiments showed that vulnerability to ant predation decreased with increase in larval size. The present study showed that predatory ants are not as relevant as demonstrated for other systems, and also illustrates how observational data and field experiments can contribute to a better understanding of the biology and ecology of a species of interest.     

LX.—Copepoda and Cladocera of the Cuillin Hills of the Isle of Skye

Summary

The life history of the African grasshopper Ornithacris turbida (Walker) was studied under controlled laboratory conditions. No phase variation was observed between isolated and crowded stock in respect of colour, morphometrics, weight of hatchlings or fledglings. All the females reared in isolation Had seven nymphal instars but in the case of the isolated males the majority had six but some had seven nymphal instars. This variation was also observed in the crowded stock for both males and females.

In isolation the mean nymphal life of 15 females was 78·7 ± 3·2 days, the mean for nine males having six instars was 59·9 ± 3·2 days and for five males having seven instars the mean nymphal life was 74·6 ± 3·8 days. Overall the nymphal life for the insects reared in crowds was slightly shorter, but no attempt was made to separate the insects having six or seven instars.

The stripes in the compound eye correlated with the number of nymphal instars. Colour changes in the compound eye coincided with overall body colour changes and maturation.

The mean maturation period of females reared in isolation was 101 days, varying from 72 to 158 days. The mean maturation period of the crowded females, estimated by the collection of the first egg-pod, was 73 days with a range of 62 to 84 days; when estimated by the collection of egg-pods averaging one per female it varied with the cage from 91 to 114 days. This suggests that maturation in the crowded females was quicker.

The isolated females lived longer than ones reared in crowded conditions, and laid more egg-pods. There was, however, no difference in the number of eggs per pod or their hatchability between crowded and isolated stock. In both cases 76% of the total number of eggs hatched. Hence overall the isolated females had a greater fecundity than those reared in crowds.

The average weight of a single hopper from isolated parents was 15·5 mg and that from crowded parents was 14·0 mg.     

The occurrence of an additional instar in the development of Chorthippus brunneus (Orthoptera: Gomphocerinae)

In two populations of Chorthippus brunneus in the Breckland district of East Anglia an additional instar is interposed between instars II and III during the development of female nymphs. The IIa nymph occurs with a frequency approaching 100%, in both heavily and very lightly grazed grasslands.

The IIa nymph is intermediate in morphological characteristics between the second and third instars. It is characterized by having wing buds similar to those of a second instar except with more extensive venation, while the genitalia are more like those of a third-instar nymph. The pronotum length: head length ratio is approximately 1·0 and there are 16 or more annuli in the antennae.

Members of the population taken from the very lightly grazed grassland and raised in the laboratory on grass that had been heavily grazed by rabbits omitted the IIa instar and so passed through only four stadia during their development. In contrast, members of the population taken from the heavily grazed field site continued to include the IIa instar in their development when fed in the laboratory on grazed grass.

Comparisons with other studies of this species show that there is geographical variation with respect to numbers of instars passed through during development. The additional instar has been found only in East Anglia where the warmer sunnier climate, which is semi-continental in the Breckland district, leads to earlier hatching. This allows more time for an extra instar to be included in the development.

The ecological implications of the observed variation in life history are discussed.