Association of viral ribonucleoprotein with polysomes

() 6 . , (=1,41, 1,45, 1,49 1,55 g/cm³). .     

Preferential excretion of glycated albumin in C57BL-Ks-J mice: effects of diabetes.

Urinary excretion of glycated albumin was quantitated in genetically hyperglycemic mice (C57BL-Ks-J, db/db mice), a model for non-insulin-dependent diabetes mellitus, and compared with their non-diabetic littermates. The data indicated a preferential excretion of glycated albumin in non-diabetic mice. This phenomenon of 'editing' of glycated albumin is decreased significantly in diabetic mice. Quantitative measurements of overall excretion of glycated albumin suggested that the loss of editing in diabetic mice is due to the dilution of glycated albumin by the unmodified albumin which is excreted in large amounts in diabetic mice. Therefore, the loss of editing observed in this model resembled the one we characterized in insulin-dependent diabetic humans and a streptozotocin-diabetic rat model.     

Separation of rat liver mitochondrial amine oxidases

.     

Magnug reflexex of the chest musculature in man

, , .     

Inhibition of sodium transport in frog skin by cardiac glycosides of different molecular structure

p 19-OM no- . ; — .     

The effect of chloramphenicol analogs on protein biosynthesis in a cell-freeEscherichia coli B system

a aaa a aa aa a a E. coli aa, a aa aa a , a a aa - a .

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Laboratory of Biological Investigations.

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Laboratory for Chemistry of Antibiotics.     

Antihypertensive effect of Co-factors of the synthesis and precursors of nucleic acids in experimental hypertension

, ₁₂, .     

A new synthetic method of coumestan derivatives

Zusammenfassung Die Synthese von 7-Hydroxy-5,6-dimethoxybenzofurano[3,2:3,4]coumarin aus 7,2-Dihydroxy-oder 7-Benzyloxy-2-hydroxy-4,5-dimethoxyisoflavon wird beschrieben.     

Effect of some monoamine oxidase inhibitors on deamination of biogenic monoamines by rat liver mitochondrial monoamine oxidase

N--N- MAO , , .     

Zur Frage der glomerulären Permeation höhermolekularer Proteine

Summary Haptoglobin and isoagglutinin were found in the urine of diabetic patients, and their renal excretion was higher in patients with diabetic angiopathy. It is concluded from these results (the biological activity of the two polypeptides in urine was intact) that the mechanism of proteinuria can perhaps be explained only by an extension of pores of the renal glomerular filter.     

The effect of protein biosynthesis inhibitors upon the development of cellular membranes hyperpolarization

( , , ) .     

MultipleW chromosome in a sea snake,Enhydrina schistosa daudin

Résumé Un serpent marin (Enhydrina chistosa Daudin, Hydrophiidae) a 32 chromosomes chez le , 33 chez la , cette dernière ayant unW-chromosome supplémentaire. La digamétie serait: -ZZ; Z W ₁W₂.

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The author is indebted to Prof. S.P. RayChaudhuri for guidance and the University Grants Commission, India, for financial assistance.     

Real-time models of morphogenetic processes

Summary Morphological transformations are often describable by simple series kinetic models,AB, ABC, etc., which allow assessment of the rates of interconversion of the distinguishable shapes or forms present and their probabilities of occurrence at various points in time, thus providing a means for kinetic comparisons with biochemical measurements of the molecular-level reactions that cause the transformations. When changes in cell morphology are followed turbidimetrically, the real-time progress curves can be simulated by fitting the data to a form of Beer's law for scattering by mixtures in which the species concentrations change with time in accordance with the chosen kinetic scheme. Because many even relatively large cells are mostly water, classical light scattering theory can be used to interpret the turbidimetric data in terms of simple geometrical models ofaverage cell size and shape suggested by microscopic examination. Two examples are briefly considered, the stimulus-induced changes in blood platelet shape and apparent size and their correlation with cytosolic-free calcium, and apparent swimming motion exhibited by neutrophils in suspension.What do you know about this business? the King said to Alice. Nothing, said Alice. Nothing whatever? persisted the King. Nothing whatever, said Alice. That's very important, the King said. Lewis Carroll (1865), in Alice's Adventures in Wonderland     

Synthesis and pharmacological properties of [1-N4-dimethyl-asparagine]-angiotensin II

Summary [1-N⁴-Dimethyl-asparagine]-angiotensin II was synthesized by Merrifield's solid-phase procedure. The analogue gave rat blood pressure about 70% relative potency to Hypertensin (Ciba). Rabbit aorta strips gave intrinsic activity _E=1, a PD₂ of 6.92±0.09 and an affinity relative to [Asn¹]-angiotensin II of 6.5%.The editors do not hold themselves responsible for the opinions expressed in the authors' brief reports. — Les auteurs sont seuls responsables des opinions exprimées dans ces brèves communications. — Für die Kurzmitteilungen ist ausschliesslich der Autor verantwortlich. — Per le brevi comunicazioni è responsabile solo l'autore. — . — Solo los autores son responsables de las opiniones expresadas en estas comunicationes breves.Acknowledgment. The authors wish to express their appreciation to Dr Emanuel Escher, School of Medicine, Centre Hospitalier Universitaire, Sherbrooke, Quebec, for his help in providing the biological data and critical comments.     

Consideration on the effects of pollution at community and population level

Summary The effects of pollution at population level are considered in relation to demographic characteristics and overall to the birthrate. The direct and indirect effects of pollution on community structure is discussed. The influence of pollution may vary according to the food-chain structure; (as a consequence, the hazard ceteris paribus will be greater in freshwater communities than in marine ones). Relations between diversity and stability are discussed. In addition, the advantages and difficulties in using diversity and biotic indices for monitoring polluted water are taken into account.     

Changes in the activities of protein kinase modulators in the cerebellum of mice due to ethanol,caffeine, or phenobarbital administration

Summary Decreased activities of both the inhibitory modulator of adenosine 35-monophosphate (cAMP)-dependent protein kinase (A-PK) as well as the stimulatory modulator of guanosine 35-monophosphate (cGMP)-dependent protein kinase (G-PK) from the mouse cerebellum were noted due to the administration of excessive doses of ethanol, caffeine, and phenobarbital for up to 28 days. The dose-dependence of the inhibition of A-PK or the stimulation of G-PK was observed as a function of the amount of protein kinase modulators in the cerebellum of mice receiving different doses of ethanol.Acknowledgments. This work was supported by a grant (RR 08119-06-PK modulator project) from NIH, USA. J.L. Williams, T. Floyd-Jones, C.F. Duggans, D.L. Boone, and S.O. Smith were prebaccalaureate trainees of MBS program. Authors thank Dr J.F. Kuo of Emory University for encouragement in this project.     

Alkaline phosphatase in human lymphocyte subpopulations

Summary The levels of membrane alkaline phosphatase have been measured on different lymphocyte fractions from human peripheral blood separated on bovine serum albumin discontinuous gradients. The peak in enzyme activity was observed in a non-T-, non-B-cell fraction, rich in null lymphocytes; the lowest values were found in the fraction with the highest proportion of T-cells.     

Biochemical evidence for two types of noradrenaline storage particles in rabbit iris

Summary Centrifugation techniques were used to determine the subcellular distribution of noradrenaline (NA) and dopamine -hydroxylase (DH) in the rabbit iris. By application of isopycnic and differential gradient centrifugation methods 2 types of NA vesicles could be demonstrated. Of the total particle bound NA about 70% is associated with light and about 30% with heavy vesicles. For both types of vesicles the distribution of DH reflected that of NA.     

Differential expression and dosage compensation of theα-amylase gene inDrosphila miranda

Summary The-amylase gene ofDrosophila miranda is located on the X²-and on the neo-Y-chromosome, both developing sex chromosomes. Crosses between strains carrying different electrophoretically distinguishable alleles of the-amylase gene were performed. Females of the F₁ offspring showed the expected heterozygosity, while the males proved to be hemizygous for this locus. Only the gene on the X²-chromosome is expressed, whereas the corresponding gene on the neo-Y-chromosome is not. Estimates of the-amylase activity in crude homogenates of male and female flies suggest strongly that the-amylase gene is dosage compensated inD. miranda. In contrast to this situation, in all otherDrosophila species the-amylase allele is autosomal and hence not dosage compensated.Acknowledgments. We would like to thank Betty C. Moore, for the kind supply ofD. miranda strains. For the help and advice in the electrophoretic separation of the-amylase variants we are indebted to Dr W. Pinsker. This work was supported by a grant from the Fonds zur Förderung der wissenschaftlichen Forschung, P5413 (Austria).     

Orally administered melatonin stimulates the 3α/β-hydroxysteroid oxidoreductase but not the 5α-reductase in the ventral prostate and seminal vesicles of pinealectomized rats

Summary Pinealectomized rats were treated orally with melatonin (MEL) for 14 days. Prostates and seminal vesicles were investigated for the activity of the 4-3-ketosteroid-5-oxidoreductase (5-R) and of the 3/-hydroxysteroid-oxidoreductase (3-HSO). The activity of the 3a-HSO was significantly stimulated (p<0.01, Friedman test) when compared to controls. The activity of the 5-R did not change significantly.This work was supported by the Deutsche Forschungsgemeinschaft, Sonderforschungsbereich 34 (Endokrinologie).