苹果原花青素对成骨细胞氧化损伤的保护作用

观察苹果原花青素对过氧化氢(H2O2)引起的成骨样细胞MC3T3-E1氧化损伤的保护作用。培养成骨样细胞MC3T3-E1,加入不同浓度的原花青素(20,30,40,50μg/mL),及H2O2(100,200,300,400μmol/L),采用MTT比色试验法观察MC3T3-E1的增殖情况;以40μg/mL的原花青素预处理细胞30 min,后加入300μmol/L H2O24 h,采用EdU试剂盒,碱性磷酸酶(ALP)活性检测法,活性氧(ROS)检测法,超氧化物歧化酶(SOD)活性检测法以及肿瘤坏死因子α(TNF-α)的ELISA检测法,观察原花青素对H2O2引起的成骨样细胞MC3T3-E1氧化损伤的保护作用。H2O2作用后,MC3T3-E1细胞的增殖受到抑制,其所产生的碱性磷酸酶(ALP)活性、SOD活性降低,而ROS与TNF-α浓度明显升高,但预先30 min给予细胞原花青素,就可以有效逆转这一趋势,苹果原花青素可有效减轻过H2O2引起的成骨样细胞MC3T3-E1氧化损伤。苹果原花青素有潜在的治疗骨质疏松症作用。     

刺老苞根皮黄酮对H_2O_2诱导的MC3T3-E1成骨细胞损伤改善

在H2O2胁迫下,研究刺老苞根皮(Aralia echinocauIis)黄酮增强MC3T3-E1成骨细胞抗自由基损伤的作用.为此用H2O2作用MC3T3-E1成骨细胞,建立自由基损伤细胞模型.培养的成骨细胞分为对照组、模型组、刺老苞根皮黄酮低剂量组(1×10-8mol/L)、刺老苞根皮黄酮中剂量组(1×10-7mol/L)和刺老苞根皮黄酮高剂量组(1×10-6mol/L).测定不同处理组细胞活力、超氧化物歧化酶(superoxide dismutase,SOD)活性、活性自由氧(reactive oxygen species,ROS)含量、脂质过氧化物(lipid oxygen,LPO)含量,同时利用荧光偏振法测定细胞膜流动性.结果显示与模型组相比,刺老苞根皮黄酮组细胞活力、SOD活性、细胞膜流动性均显著升高(P<0.01),而ROS含量、LPO含量则显著降低(P<0.01),并呈现一定的量效关系.可以认为H2O2摄入会导致MC3T3-E1成骨细胞的氧化损伤,而刺老苞根皮黄酮可以预防或降低此类损伤对细胞的影响.     

绿原酸对体外培养成骨细胞活性的影响

为研究接骨草成分之一绿原酸(CGA)对成骨细胞MC3T3-E1活性的影响,采用MTT法检测11.02,22.05,44.09,88.19μmol/L CGA对成骨细胞增殖率,碱性磷酸酶(ALP)试剂盒检测成骨细胞ALP活性,实时定量PCR检测成骨细胞骨相关基因的表达.结果表明:11.02～88.19μmol/L CGA对成骨细胞增殖具有明显的促进作用.培养2d,44.09μmol/L CGA能促进ALP表达上调;22.05,44.09μmol/L CGA能促进ALP、Runx2、Osterix、c-jun和c-fos基因的表达;培养6 d,44.09μmol/L CGA能促进c-fos基因的表达;在整个培养过程中,I型胶原(Col-I)基因的表达具有浓度和时间依赖性.故CGA具有一定的成骨活性,是接骨草的成骨活性成分之一.     

淫羊藿中有效成分淫羊藿甙分离纯化的研究

在Tween80-(NH4)2SO4液-固萃取体系中,试验了淫羊藿甙在两相的分配行为.选择Tween80浓度为9%,(NH4)2SO4浓度为1.667mol/L,萃取pH4.20,淫羊藿甙的一次萃取率可达95.2%.选择(NH4)2SO4浓度为2.121mol/L,反萃pH10.30,经一次反萃,可使94.2%的淫羊藿甙与Tween80分离.用HPLC对分离效果作了评价,结果表明,该萃取体系对淫羊藿粗提液有明显的分离纯化作用.     

高效液相色谱化学发光法测定淫羊藿甙

在碱性条件下,研究了淫羊藿甙与Co(Ⅱ)形成的1∶1配合物催化鲁米诺-H2O2体系产生化学发光.在此基础上建立了反相高效液相色谱(RP-HPLC)分离柱后化学发光检测淫羊藿甙的新方法,并成功应用于安神补脑液中淫羊藿甙的测定.方法在0.5~20μg/mL范围内与化学发光强度呈良好的线性关系,相关系数为0.9986,检出限为2×10-7g/mL(3σ).对2×10-6g/mL淫羊藿甙平行测定11次,其相对标准偏差为4.5%.     

氧化石墨烯/磷酸钙生物水泥体外生物相容性研究

等摩尔的Ca_3(PO_4)2-CaHPO_4-CaCO_4(CPCs)水泥系为固相,不同质量浓度的氧化石墨烯(GO)水分散液(0~24mg/mL)为液相,按一定的固液比固化,得到复合材料GO/CPCs.以成骨细胞MC3T3-E1为对象,初步探究该复合材料试样的体外生物相容性.扫描电镜观察细胞贴壁形态,MTT法评价细胞活力,碱性磷酸酶(ALP)活性检测探讨材料的骨诱导性.结果显示:MC_3T3-E1细胞在材料表面状态良好,产生胞质突;GO的添加能够增强MC3T3-E1细胞活力;GO/CPCs在短期内(1d)促进MC3T3-E1细胞早期分化.GO/CPCs有望成为一种新型骨组织替代材料.     

湘西地区不同居群箭叶淫羊藿叶片的脂质过氧化

对湘西地区凤凰、永顺、龙山3个不同居群的箭叶淫羊藿叶片的超氧阴离子自由基（·O₂）的产生速率和丙二醛（MDA）的含量进行测定.结果表明：永顺的箭叶淫羊藿的超氧阴离子自由基的产生速率最大,为(0.059　1±0.003　3) μmol·min^-1·g^-1,而龙山的箭叶淫羊藿的超氧阴离子自由基的产生速率最小,为(0.053　0±0.000　9) μmol·min^-1·g^-1;在3个不同居群的箭叶淫羊藿叶片的MDA的含量中,永顺的箭叶淫羊藿的含量最高,为(0.014 9±0.001 4) nmol·g^-1,而龙山的箭叶淫羊藿的含量最低,为(0.007　5±0.001　4) nmol·g^-1.     

玉柏石松中甾体化合物对体外培养成骨细胞活性的影响

为研究玉柏石松中甾体化合物豆甾烷-3-酮-21-羧酸（SA）对体外培养小鼠成骨细胞系MC3T3-E1活性的影响,用Alamar Blue法检测了成骨细胞增殖率,碱性磷酸酶试剂盒检测了细胞中碱性磷酸酶活性,茜素红染色检测了成骨细胞矿化水平,荧光定量PCR检测了成骨细胞骨分化相关基因的表达.结果显示：8μmol/L和16μmol/L的SA处理细胞8 d能抑制成骨细胞碱性磷酸活性;处理细胞16 d能提高骨细胞矿化水平.SA抑制成骨早期分化相关基因（Runx-2和Osterix）的表达,促进骨基质蛋白OPN和骨重建相关转录因子（Jun-D,Fra-1和Fra-2）的表达.故SA具有促进骨折愈合的成骨活性,可能通过促进相关转录因子表达,骨折断面旧骨的吸收和骨基质钙化等方式完成.     

玉柏石松中甾体化合物对体外培养成骨细胞活性的影响

为研究玉柏石松中甾体化合物豆甾烷-3-酮-21-羧酸(SA)对体外培养小鼠成骨细胞系MC3T3-E1活性的影响,用Alamar Blue法检测了成骨细胞增殖率,碱性磷酸酶试剂盒检测了细胞中碱性磷酸酶活性,茜素红染色检测了成骨细胞矿化水平,荧光定量PCR检测了成骨细胞骨分化相关基因的表达.结果显示:8μmol/L和16μmol/L的SA处理细胞8 d能抑制成骨细胞碱性磷酸活性;处理细胞16 d能提高骨细胞矿化水平.SA抑制成骨早期分化相关基因(Runx-2和Osterix)的表达,促进骨基质蛋白OPN和骨重建相关转录因子(Jun-D,Fra-1和Fra-2)的表达.故SA具有促进骨折愈合的成骨活性,可能通过促进相关转录因子表达,骨折断面旧骨的吸收和骨基质钙化等方式完成.     

低强度脉冲超声对MC3T3-E1细胞增殖、分化和矿化的影响

 为了研究低强度脉冲超声促进MC3T3-E1细胞增殖、分化和矿化的有效性,初步筛选频率和强度参数,分别对细胞施加不同参数(频率、强度)的超声,采取CCK-8法检测细胞的增殖情况,碱性磷酸酶检测试剂盒检测分化效果,茜素红染色观察矿化效果。发现30mW/cm²和40mW/cm²的超声对细胞产生促增殖作用,50mW/cm²抑制增殖;1.5MHz和1.7MHz的较低强度组均较对照组的ALP活性有增加;1.5MHz,40mW/cm²的超声组矿化效果较其他组好。研究结果表明,低强度的脉冲超声可以提高成骨细胞株MC3T3-E1增殖、分化和矿化能力,可能是防治骨质疏松的可行手段之一。     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Lower Paleozoic oil relationships within Williston Basin, Canada

The Williston Basin is a significant petroleum province, containing oil production zones that include the Middle Cambrian to Lower Ordovician, Upper Ordovician, Middle Devonian, Upper Devonian and Mississippian and within the Jurassic and Cretaceous. The oils of the Williston Basin exhibit a wide range of geochemical characteristics defined as "oil families", although the geochemical signature of the Cambrian Deadwood Formation and Lower Ordovician Winnipeg reservoired oils does not match any "oil family". Despite their close stratigraphic proximity, it is evident that the oils of the Lower Palaeozoic within the Williston Basin are distinct. This suggests the presence of a new "oil family" within the Williston Basin. Diagnostic geochemical signatures occur in the gasoline range chromatograms, within saturate fraction gas chromatograms and biomarker fingerprints. However, some of the established criteria and cross-plots that are currently used to segregate oils into distinct genetic families within the basin do not always meet with success, particularly when applied to the Lower Palaeozoic oils of the Deadwood and Winnipeg Formation.     

An Analysis of Catherine＇s Character——Wuthering Heights

Wuthering Heights, Emily Bronte＇s only novel, was published in December of 1847 under the pseudonym Ellis Bell. The book did not gain immediate success, but it is now thought one of the finest novels in the English language. Catherine is the key character of this masterpiece, because everybody and everything center on her though she had a short life. We can understand this masterpiece better if we know Catherine well.     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

Design and analysis of generic LBS application developing platform

Location based services is promising due to its novel working style and contents.A software platform is proposed to provide application programs of typical location based services and support new applications developing efficiently. The analysis shows that this scheme is easy implemented, low cost and adapt to all kinds of mobile nework system.     

橡胶颗粒沥青混合料低温抗裂性试验研究

以AC-13级配为基础,将橡胶颗粒代替部分集料掺入混合料中,以低温弯曲试验为评价方法对不同橡胶颗粒掺量下沥青混合料的低温抗裂性进行研究,并引入应变能密度值对混合料的低温抗裂性进行综合评价.试验结果表明:橡胶颗粒沥青混合料试件的破坏微应变均超过2 300,满足冬寒区的技术指标;无论是否掺加橡胶颗粒,随着温度的下降,沥青混合料破坏时的最大弯拉强度增大,弯拉应变降低,劲度模量增大;弯曲应变能密度在胶粒掺量为1%左右时具有较大的弯曲应变能密度值,此时橡胶颗粒沥青混合料具有较好的低温抗裂性.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

Quantitative trait loci （QTLs） for quality traits related to protein and starch in wheat

Quality traits in wheat （Triticum aestirum L.） were studied by quantitative trait locus （QTL） analysis in a recombinant inbred line （RIL） population, a set of 131 lines derived from Chuan 35050 × Shannong 483 cross （ChSh）. Grains from RILs were assayed for 21 quality traits related to protein and starch. A total of 35 putative QTLs for 19 traits with a single QTL explaining 7.99-40.52% of phenotypic variations were detected on 10 chromosomes, 1D, 2A, 2D, 3B, 3D, 5A, 6A, 6B, 6D, and 7B. The additive effects of 30 QTLs were positive, contributed by Chuan 35050, the remaining 5 QTLs were negative with the additive effect contributed by Shannong 483. For protein traits, 15 QTLs were obtained and most of them were located on chromosomes 1 D, 3B and 6D, while 20 QTLs for starch traits were detected and most of them were located on chromosomes 3D, 6B and 7B. Only 7 QTLs for protein and starch traits were co-located in three regions on chromosomes 1D, 2A and 2D. These protein and starch trait QTLs showed a distinct distribution pattern in certain regions and chromosomes. Twenty-two QTLs were clustered in 6 regions of 5 chromosomes. Two QTL clusters for protein traits were located on chromosomes 1D and 3B, respectively, three clusters for starch traits on chromosomes 3D, 6B and 7B, and one cluster including protein and starch traits on chromosome 1D.     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

A computer generator for randomly layered structures

AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…