新疆杏品种自交不亲和S-RNase基因PCR扩增体系的优化

以3个新疆杏品种的叶片为试材,以其叶片基因组DNA为模板,分别对PCR扩增体系中的10×Buffer(含Mg2+)、dNTP、上下游引物、TaqDNA聚合酶和退火温度5个因素设计6个梯度,研究了新疆杏品种自交不亲和S-RNase基因PCR扩增条件,建立其优化的PCR扩增体系。研究结果表明:其扩增程序为94℃预变性5min,94℃变性45s,52℃退火45s,72℃延伸2min,35个循环后72℃延伸10min,25μL反应体系10×Buffer(含Mg2+)2.5μL,dNTP0.08mmol/L,上下引物0.16μmol/L,DNA80ng,DNA聚合酶1μL,此为最优的反应体系。     

河北省仁用杏生产现状与趋势分析

<正>仁用杏原产于我国,是以杏仁为主要产品的杏属果树的总称,包括苦仁和甜仁两大类,苦仁类主要指西伯利亚杏[Armeniaca sibirica(L.)Lam]、辽杏[A.Mandshurica(Maxim)Skv.]、藏杏[A.holosericea(Batal.)Kost.]和普通杏[A.valqaris Lam.]的野生类型;甜仁类主要指普通杏和西伯利亚杏种间自然杂交的杏扁系列品种~([1])。仁用杏是我国重要的经济林树种,是食品工业和医药工业的重要原料。杏树     

巴旦杏壳天然黄色素提取及其稳定性研究

巴旦杏壳是巴旦杏加工过程中的废弃物,利用水/乙醇为浸提剂,在75℃,浸提时间为2 h,料液比为1 g∶15 mL条件下,可提取巴旦杏壳黄色素及木质素。对提取出的黄色素稳定性进行了研究,结果表明,杏壳黄色素在避光条件下的稳定性较好,日光照射会影响黄色素稳定性;杏壳黄色素在一定温度下稳定性较好,但当温度达到85℃时,吸光度明显下降;蔗糖、pH对杏壳黄色素稳定性有影响;Na+、K、Ca2+、Mg2+等金属离子对杏壳黄色素稳定性没有明显影响,而Cu2+、Al3+对杏壳黄色素稳定性影响较大;氧化剂对杏壳黄色素稳定性影响较大;还原剂Vc对杏壳色素稳定性影响不大。     

西伯利亚杏(Prunus sibirica)的水分关系

前言西伯利亚杏(Prunus sibirica L.)主要分布在森林草原带,常成为山地灌丛和灌木草原的优势种和景观植物;在典型草原带的沙地上也有分布,并常常集中地分布在固定沙丘阳坡上,形成独特的沙地灌丛。西伯利亚杏属于旱中生灌木或小乔木,耐寒性强,又能抗旱,五月份开花,粉红色花     

耐寒植物叶片比热参数的测定

采用DSC差式扫描量热仪测定不同温度下耐冬（Common Camellia）、大叶冬青（Ilexlatifolia Thunb．）和碧玉（Fes．Rub．ew．Jas．）3种植物叶片的比热容,并得到不同温度范围内叶片比热容与温度间的近似关系式。通过对比分析比热容曲线得出结论：耐寒植物叶片比热在-4～0℃温度范围内快速增长,在0℃达到峰值,在0～2℃温度范围内急剧下降,随后比热容随着温度升高而小幅度上升;虽然耐寒植物叶片比热容曲线相似,但不同耐寒性植物叶片其比热容峰值差异较大;非耐寒植物叶片的比热容在生长温度范围内呈二次函数关系平缓增加。     

制干温度对“果汁杏干”品质的影响

针对不同干制温度导致杏干果实发生褐变、水分含量不同、质地和感官品质降低等问题,探究不同制干温度对"果汁杏干"品质的影响,以期得出杏干最佳制干温度.以鲜杏为材料,通过设置不同温度(40℃,45℃,50℃,55℃,60℃)对杏进行低温烘烤,研究在不同制干温度下,杏干水分含量、生理指标及感官品质的变化.结果表明,处理温度在55℃时,通过维持杏干的水分含量在16%左右,可以较好地抑制杏干在干制期间总色差的升高,减缓总酸含量、抗坏血酸(Vc)含量、还原糖含量的下降,并且可以较好地维持杏干的营养品质和感官品质.     

龙眼抗热性的研究

以晚熟龙眼品种立冬本为材料,研究其在25、35、40C温度下叶片的过氧化物酶(POD)活性、叶绿素a(chla)、叶绿素b(chlb)、丙二醛(MDA)活性和土壤含水量的变化.研究结果表明chla随着温度的上升呈下降趋势,chlb较稳定、因而导致chla/b的下降;35℃处理的土壤失重率最大,叶片丙二醛(MDA)含量高、过氧化物酶(POD)活性最强;40℃处理其次;龙眼在40℃处理条件下出现叶片失绿、反卷等不良反应,龙眼已开始受到高温危害.     

高温影响水稻叶片气孔开闭的扫描电镜观察

本文对不同温度处理的水稻叶片进行了扫描电镜砚察,以考察温度对水稻叶片气孔形态变化的影响。结果表明,在29—33℃范围内,温度对气孔的开度影响不大。33—41℃范围内,气孔的开度与处理温度之间呈显著负相关。41℃时,水稻叶片气孔几乎完全关闭。扫描电镜观察,还发现了杂交水稻气孔的特殊构造——“门闩”结构。     

等温校正温度对TC11合金叶片型面及金相组织的影响研究

通过对TC11合金叶片在900℃,860℃,820℃时的等温下进行试验研究,得出在等温热校形过程中,随着校正温度的提高,叶片校正效果越来越明显,当校正温度提高到900℃时,选取的试验叶片等温校正型面最好,校正效果最佳,高低倍金相组织均匀。     

高Nb--TiAl合金叶片锻造模拟

采用Deform-3D有限元软件模拟β-γ高Nb-TiAl合金叶片等温锻造,分析等效应变场、等效应力场与温度场的分布.叶片等温锻造中叶身与榫头的等效应变分布均匀,随着上模具压下速度的增大和预热温度的升高,变形过程中等效应力降低,有利于动态再结晶的发生;上模具压下速度在1.0~1.5 mm·s-1、预热温度在1250~1300℃有利于提高β-γ高Nb-TiAl合金叶片锻件的质量.     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Lower Paleozoic oil relationships within Williston Basin, Canada

The Williston Basin is a significant petroleum province, containing oil production zones that include the Middle Cambrian to Lower Ordovician, Upper Ordovician, Middle Devonian, Upper Devonian and Mississippian and within the Jurassic and Cretaceous. The oils of the Williston Basin exhibit a wide range of geochemical characteristics defined as "oil families", although the geochemical signature of the Cambrian Deadwood Formation and Lower Ordovician Winnipeg reservoired oils does not match any "oil family". Despite their close stratigraphic proximity, it is evident that the oils of the Lower Palaeozoic within the Williston Basin are distinct. This suggests the presence of a new "oil family" within the Williston Basin. Diagnostic geochemical signatures occur in the gasoline range chromatograms, within saturate fraction gas chromatograms and biomarker fingerprints. However, some of the established criteria and cross-plots that are currently used to segregate oils into distinct genetic families within the basin do not always meet with success, particularly when applied to the Lower Palaeozoic oils of the Deadwood and Winnipeg Formation.     

An Analysis of Catherine＇s Character——Wuthering Heights

Wuthering Heights, Emily Bronte＇s only novel, was published in December of 1847 under the pseudonym Ellis Bell. The book did not gain immediate success, but it is now thought one of the finest novels in the English language. Catherine is the key character of this masterpiece, because everybody and everything center on her though she had a short life. We can understand this masterpiece better if we know Catherine well.     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

Design and analysis of generic LBS application developing platform

Location based services is promising due to its novel working style and contents.A software platform is proposed to provide application programs of typical location based services and support new applications developing efficiently. The analysis shows that this scheme is easy implemented, low cost and adapt to all kinds of mobile nework system.     

橡胶颗粒沥青混合料低温抗裂性试验研究

以AC-13级配为基础,将橡胶颗粒代替部分集料掺入混合料中,以低温弯曲试验为评价方法对不同橡胶颗粒掺量下沥青混合料的低温抗裂性进行研究,并引入应变能密度值对混合料的低温抗裂性进行综合评价.试验结果表明:橡胶颗粒沥青混合料试件的破坏微应变均超过2 300,满足冬寒区的技术指标;无论是否掺加橡胶颗粒,随着温度的下降,沥青混合料破坏时的最大弯拉强度增大,弯拉应变降低,劲度模量增大;弯曲应变能密度在胶粒掺量为1%左右时具有较大的弯曲应变能密度值,此时橡胶颗粒沥青混合料具有较好的低温抗裂性.     

A computer generator for randomly layered structures

AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

Quantitative trait loci （QTLs） for quality traits related to protein and starch in wheat

Quality traits in wheat （Triticum aestirum L.） were studied by quantitative trait locus （QTL） analysis in a recombinant inbred line （RIL） population, a set of 131 lines derived from Chuan 35050 × Shannong 483 cross （ChSh）. Grains from RILs were assayed for 21 quality traits related to protein and starch. A total of 35 putative QTLs for 19 traits with a single QTL explaining 7.99-40.52% of phenotypic variations were detected on 10 chromosomes, 1D, 2A, 2D, 3B, 3D, 5A, 6A, 6B, 6D, and 7B. The additive effects of 30 QTLs were positive, contributed by Chuan 35050, the remaining 5 QTLs were negative with the additive effect contributed by Shannong 483. For protein traits, 15 QTLs were obtained and most of them were located on chromosomes 1 D, 3B and 6D, while 20 QTLs for starch traits were detected and most of them were located on chromosomes 3D, 6B and 7B. Only 7 QTLs for protein and starch traits were co-located in three regions on chromosomes 1D, 2A and 2D. These protein and starch trait QTLs showed a distinct distribution pattern in certain regions and chromosomes. Twenty-two QTLs were clustered in 6 regions of 5 chromosomes. Two QTL clusters for protein traits were located on chromosomes 1D and 3B, respectively, three clusters for starch traits on chromosomes 3D, 6B and 7B, and one cluster including protein and starch traits on chromosome 1D.     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.