解钾硅酸盐细菌培养基及培养条件的研究

以所筛选的硅酸盐细菌RM 0 1菌株为材料 ,对所需的培养基和培养条件进行了研究分析 .在选择的适宜培养基中 (TR复合培养基 ) ,对培养基pH值、培养温度、摇床转速及摇瓶装液量等影响因素进行了正交实验 .结果表明 ,在pH为 7、温度 36℃、转速 2 2 0r/min、装液量 5 0mL时 ;硅酸盐细菌菌量可达 10 .6× 10 8个 /mL     

苏云金芽孢杆菌在固态和液态基质中的生长差别研究

在同等条件下,采用同一培养基的固态和液态两种环境,对苏云金芽孢杆菌中国标准品(BtCS3ab-1991)的发酵质量进行了比较研究.结果表明:固态发酵培养的Bt芽孢平均长度0.028mm,液态发酵平均为0.017mm,含菌量在28h分别为0.88×1010个/l和0.54×1010个/l.发酵过程中菌体浓度在生长曲线上表现为固态发酵高于液态,缩短发酵时间2h,且接种量对固态发酵的影响也较小.而在虫害研究方面,取食含固态药液菜叶的小菜蛾羽化率较液体降低10%,死亡率差异显著.     

唾液链球菌的培养工艺研究

对唾液链球菌的固体和液体培养条件进行优化,通过单因素试验和参考已有文献资料设计了不同的培养条件,优化得到了唾液链球菌固体培养最优的条件是:MRS培养基,pH 7.2,温度37℃下在体积分数为5%的CO2的培养箱中培养。液体培养条件是:按照乳糖7 g,蔗糖3.5 g,葡萄糖3.5 g,胰蛋白胨14 g,大豆蛋白胨7 g,酵母提取物7 g,磷酸氢二钾/磷酸二氢钾0.02mol/L,硫酸镁200 mg/L,硫酸锰100 mg/L,氯化钙60 mg/L,VitB110 mg/L,蒸馏水1L的比例配制液体培养基,pH 7.2,温度37℃下在体积分数为5%的CO2的培养箱中静置培养。进行放大培养后,最终得到的冻干好的菌粉共计10.9 g,活菌数达到4.9×1011cfu/g。为工业生产提供了有效的信息。     

多粘类芽孢杆菌培养条件优化研究

对多粘类芽孢杆菌菌株B-306的培养基配方及培养条件进行优化研究,优化后的多粘类芽孢杆菌B-306培养基配方及培养条件为:可溶性淀粉17.5 g·L-1、蛋白胨25.0 g·L-1、NaCl 1.25 g·L-1、Na2HPO40.5g·L-1、CaCl21.0 g·L-1,pH7.5;培养温度30℃、转速170 r·min-1、装液量80 mL/250 mL;接种量为10%(体积分数).经过以上条件优化,多粘类芽孢杆菌B-306的细胞生物量可达5.3×109cfu·mL-1,比优化前(3.17×108cfu·mL-1)提高了15.72倍.     

黄腐酸高产菌株的培养条件优化研究

对发酵蔗渣产黄腐酸(FA)的高产菌株-枯草芽孢杆菌的培养基配方及培养条件进行优化研究.获得的优化培养基配方为:可溶性淀粉、蛋白胨、磷酸氢二钾和硫酸镁的添加量分别为12.50、12.50、0.25、0.50 g·L~(-1);优化的培养条件为:pH 7.0、温度37℃、装液量20 m L/250 m L、转速130 r·min~(-1)、接种量5%(体积分数).在该优化的培养基及培养条件下,枯草芽孢杆菌培养12 h后的细胞生物量达2.280×10~9cfu·m L~(-1),较优化前提高了77.9倍.采用优化前后的条件培养菌种进行发酵产黄腐酸实验,结果优化后的发酵产品中FA含量为26.36%(质量分数),细胞生物量为1.09×10~9cfu·g~(-1),比优化前分别提高了13.9%和2.3倍.     

甜瓜枯萎病原菌的微生物学特性及其在植株体内分布

2003年于福州永泰采集甜瓜枯萎病病株,病原菌经鉴定为尖孢镰刀菌(Fusariumoxysporum),在甜瓜健株、病株体内均有分布.菌量分布依次为根茎部含量最大,茎蔓中部次之,茎蔓顶部最少.不同发病级别植株体内的平均含菌量测定结果表明:发病级别为2级的植株体内的菌量最大,为10.75×104cfu/g鲜质量,其次为发病级别为3级和1级的植株,含菌量分别为8.90×104cfu/g鲜质量和8.63×104cfu/g鲜质量;健株中的含菌量最少,仅为0.08×104cfu/g鲜质量.该尖孢镰刀菌菌丝体生长的适宜温度为25±1℃,pH为7.     

大豆为原料的根霉纤溶酶固态发酵工艺条件

研究了以大豆为原料华根霉TK317产纤溶酶固态发酵的工艺条件。采用单因素试验对固态发酵培养基的碳源、碳氮比、初始pH、加水量和温度进行了优化;采用正交试验对接种量和培养基厚度进行了研究。结果表明,实验范围内华根霉TK317固态发酵产纤溶酶的适宜培养基组成:m(面粉)∶m(大豆)=1∶9,初始pH为5.0,加水量为0.75 mL/g。适宜培养条件:培养温度为28℃,培养基厚度为15 g/250 mL三角瓶,接种量1×107个/g,培养时间为60 h。优化条件下的纤溶酶产量平均达480.52 U/g。     

菌肥用光合菌在纤维素水解液中的培养

对光合菌在天然培养基、合成培养基以及来自植物纤维素水解液的半合成培养基中的培养进行了研究 ,对适宜的培养基、营养物质的稀释度、菌种接种量、pH值等进行了选择 .对选出的 4号加有纤维素水解液的培养基 ,接入 5 %的菌液 ,pH为 7.0～ 7.5 ,温度为 2 5～ 30℃ ,厌氧条件下 ,光合菌生长良好 ,菌量达 2 .2× 10 9亿个/mL ,在加入光合菌生长促进液 (1% )后 ,菌量达 4.0 1× 10 9个 /mL .     

高产杆菌肽菌株的筛选及培养条件研究

采用细胞融合技术得到的菌株与不同来源地衣芽孢杆菌菌株,进行深层液体发酵生产杆菌肽,研究了不同菌株,不同碳源,不同量锌和磷量诸因素对杆菌肽高产的影响.结果表明,1号菌比B-1号、B-2号、3号、4号株菌的效价高,为5.80×102Iu.mL-1.以柠檬酸为碳源时,磷的用量为0.10~0.20 mmol.L-1的最佳培养基中培养时,最有利于杆菌肽的合成,锌的用量10%,其发酵液中杆菌肽效价最高,可达8.86×102~9.85×102Iu.mL-1.     

桦褐孔菌深层发酵培养条件的优化

目的优化桦褐孔菌深层发酵条件。方法采用L9(34)正交试验法对培养基进行优化。结果桑黄菌体发酵的最优方案是8%葡萄糖,1.60%酵母粉,0.3%KH2PO4,0.15%MgSO4,VB110mg/100mL,pH6.0,接种量为10%,装液量为100mL/300mL三角瓶,150r/min,恒温培养7天,生物量可达1.350g干菌丝每100mL发酵液。结论桦褐孔菌可以用深层液体发酵培养获得菌丝体,桦褐孔菌具有限氧生长的生物学特性。     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

A computer generator for randomly layered structures

AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…     

An Index mechanism for multi-scale view in spatial databases

There are numerous geometric objects stored in the spatial databases. An importance function in a spatial database is that users can browse the geometric objects as a map efficiently. Thus the spatial database should display the geometric objects users concern about swiftly onto the display window. This process includes two operations:retrieve data from database and then draw them onto screen. Accordingly, to improve the efficiency, we should try to reduce time of both retrieving object and displaying them. The former can be achieved with the aid of spatial index such as R-tree, the latter require to simplify the objects. Simplification means that objects are shown with sufficient but not with unnecessary detail which depend on the scale of browse. So the major problem is how to retrieve data at different detail level efficiently. This paper introduces the implementation of a multi-scale index in the spatial database SISP (Spatial Information Shared Platform) which is generalized from R-tree. The difference between the generalization and the R-tree lies on two facets: One is that every node and geometric object in the generalization is assigned with a importance value which denote the importance of them, and every vertex in the objects are assigned with a importance value,too. The importance value can be use to decide which data should be retrieve from disk in a query. The other difference is that geometric objects in the generalization are divided into one or more sub-blocks, and vertexes are total ordered by their importance value. With the help of the generalized R-tree, one can easily retrieve data at different detail levels.Some experiments are performed on real-life data to evaluate the performance of solutions that separately use normal spatial index and multi-scale spatial index. The results show that the solution using multi-scale index in SISP is satisfying.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that     

On the Thermodynamic Limit for Responses to Static Electromagnetic Fields

For molecular systems,the quantum-mechanical treatment of their responses to static electromagnetic fields usually employs a scalar-potential treatment of the electric field and a vector-potential treatment of the magnetic field.Although the potential for each field separately is associated with the choice of an(unphysical)origin,the precise choice of the origin for the electrostatic field has little consequences for the results.This is different for the