白腐真菌J610-D分泌漆酶处理几种有毒环境污染物的研究

就白腐真菌漆酶对环境中几种常见的有毒污染物的降解效果进行了研究.结果表明:白腐真菌漆酶能够很好地催化氧化芳胺类及其衍牛物,当控制苯胺浓度<800 mg/L、邻苯二胺浓度<150 mg/L时,白腐真菌漆酶对苯胺、邻苯二胺的降解率均可达90%、100%;白腐真菌漆酶对硝基苯类化合物的降解效果不明显,当控制其浓度<150mg/L,对硝基苯化合物的降解率可达80%.     

微生物产酶的不确定性因素确定化的一种方法

微生物产酶是一个具有不确定性的复杂过程．将数学上的关联分析思想应用于白腐菌的产酶过程,计算影响该菌产酶的四种因素的关联度．结果表明,其中含水率与白腐菌分泌木素过氧化物酶、锰过氧化物酶的关联度最大,分别达0．8310和0．7479,说明含水率是影响该菌产酶的最主要因素．此外,提出可建立GM（1,1）模型用于日产酶量的预测,为今后探索微生物产酶条件提供了方法参考．     

白腐真菌对邻苯二甲酸二甲酯的降解与机理研究

文章以黄孢原毛平革菌(Phanerochaete chrysosporium)作为白腐真菌的代表，研究其对邻苯二甲酸二甲酯(dimethyl phthalate, DMP)的降解效果与机理。实验结果表明，在最佳条件(pH=5.6,温度为30℃,DMP初始质量浓度为10 mg/L)下，7 d后DMP的去除率为75%。进一步优化反应条件发现：体系中初始葡萄糖质量浓度为5 g/L时，反应结束时DMP降解效率为95%;当反应中加入20μg/L H₂O₂或27.0 mg/L Mn²⁺,反应7 d后DMP的降解效率显著提升到100%。此外，过氧化物酶在DMP降解过程中起重要作用，实验证明DMP的降解更多地由锰过氧化物酶(manganese peroxidase, MnP)驱动；傅里叶红外光谱证实氨基、羧基、羟基等还原性官能团参与了DMP的去除。文中通过液相色谱-质谱(liquid chromatography-mass spectrometry, LC-MS)联用仪确定DMP的降解产物，并讨论3种可能的降解途径。     

白腐菌降解偶氮金属络合染料的研究

针对白腐菌的特性和独特的降解机理,用Corilus versicolor和Panus conchatus两种典型白腐菌降解金属络合染料,结果表明,白腐菌对这类染料有明显的脱色作用,经驯化的白腐菌对染料废液处理72h脱色率可达90％以上,测定了在特定条件下两种白腐菌产锰过氧化物酶（Mnp)和漆酶（Laccase)的活性,讨论了染料的脱色与酶活及菌丝产量高低的相关性,认为白腐菌对该类染料的脱色作用包括物理吸附和酶促降解两个方面。     

Pleurotus ostreatus对直接耐晒翠蓝染料溶液脱色的研究

研究Pleurotus ostreatus(白腐真菌)对直接耐晒翠蓝GL染料溶液的脱色性能.结果表明,Pleurotus ostreatus能有效用于直接耐晒翠蓝染料溶液的脱色,pH值为5、温度为22 ℃左右脱色效果最佳;C溶液/C培养基＞20%时,增大营养物浓度对脱色率无显著影响;Pleurotus ostreatu对 GL的生物吸附符合Langmuir吸附模式,饱和吸附量为20 mg/g(湿菌体);并可用Freundlich吸附模型表达,吸附动力学可用准二级速率方程描述;脱色是通过菌体吸附和菌酶降解双重机制实现的.     

铜胁迫对蚕豆种子萌发及幼苗生长的影响

不同浓度的铜溶液对蚕豆种子进行处理,观察蚕豆种子的发芽率和幼苗生长状况,以研究铜胁迫对蚕豆种子萌发及幼苗生长的影响.结果表明:铜溶液浓度低于80 mg/L时,对蚕豆种子萌发影响较小,浓度高于80 mg/L时,对种子萌发有较明显的抑制作用;铜溶液浓度低于10 mg/L时,可适当促进蚕豆幼苗的生长,可增加苗高和鲜物质量,浓度过高则会抑制其生长;铜溶液浓度低于20 mg/L时,对根系的蛋白含量、过氧化物酶的活性和叶绿素的质量分数有明显的促进作用,当铜溶液浓度高于20 mg/L时,蚕豆蛋白含量、过氧化物酶的活性和叶绿素的质量分数都逐渐减低.表明低浓度的铜处理对蚕豆生长有促进作用,最适于蚕豆生长的铜离子浓度为10 mg/L;铜离子浓度过高则会产生生理毒害作用,进而影响蚕豆种子的萌发率和幼苗的正常生长.     

固定化黄孢原毛平革菌对邻苯二甲酸 二乙酯的降解效果研究

以白腐真菌的模式菌种-黄孢原毛平革菌作为研究对象,探究固定化黄孢原毛平革菌对邻苯二甲酸二乙酯(DEP)的降解效果及其最佳降解条件.研究表明,固定化黄孢原毛平革菌相对于游离态菌体能产生较高活性的木质素过氧化物酶(Li P)和锰过氧化物酶(Mn P).DEP降解效果受固定化菌体接种量、p H值、温度和DEP初始浓度等因素的影响.当p H为6,温度为35℃,DEP初始浓度为10 mg/L时,加入8 g固定化黄孢原毛平革菌时,DEP去除效果最好,其去除率达到94.6%.     

白腐真菌对百里酚的降解机理

白腐真菌在环境治理方面有很大的应用潜力.以百里酚作为研究对象,利用白腐真菌在DOX培养基中对其进行微生物降解,通过测定降解体系中木质素过氧化物酶(LiP)、锰过氧化物酶(MnP)、漆酶(Lac)和多酚氧化酶(PPO)的活性,并利用红外光谱仪、气相色谱-质谱分析仪分析了降解产物.结果发现,百里酚在Lac和LiP催化下发生了脱酚基、脱甲基、开环、氧化等作用,得到对应降解产物,从而推测出白腐真菌对百里酚的降解历程.     

乙基紫测定人血清白蛋白的研究

研究乙基紫(EV)与人血清白蛋白(HSA)的结合反应.在pH=3.78的条件下,HSA的加入使EV在其最大吸收峰(595 nm)处吸光度增强,比EV溶液的吸光度显著增大.用紫外-可见分光光度计测定,在595 nm处EV-HSA溶液的吸光度值与EV溶液的吸光度值之差△A与HSA的浓度c在一定范围内有线性关系,线性响应范围为5.0～150.0 mg/L,检出限为0.097 1 mg/L,用于人血清白蛋白样品测定结果满意,由此建立起快速测定HSA的实用方法.该法具有简便、快速、干扰少、灵敏度高的特点.同时,初步探讨EV-HSA结合机理.     

用化学检测法测定氯硝柳胺含量

目的:在测定氯硝柳胺浓度上 ,我省一直采用生物学方法进行测定 ,不能准确测量出水体中的氯硝柳胺含量 ,现建立氯硝柳胺浓度化学测定方法。方法 :采用紫外分光光度法测定氯硝柳胺标准品和氯硝柳胺商品药 ,两样品分别配制为0.1、0.25、0.5、1.0、2.0、4.0、5.0、8.0、10.0mg/L溶液,测定两样品各浓度的吸光度值(Abs)。结果 :用氯硝柳胺标准品测定的吸光度值求出回归方程为y=0.2557x 0.0953 ,并绘制氯硝柳胺标准品工作曲线 ,氯硝柳胺标准品和商品药所测定吸光度值经统计学检验无显著性差异 (t=0.032 ,P>0.05)。结论 :氯硝柳胺标准品测定所求出的回归方程和绘制的标准曲线可用于氯硝柳胺的浓度测定 ,对灭螺、杀蚴工作将起到指导作用。     

An Index mechanism for multi-scale view in spatial databases

There are numerous geometric objects stored in the spatial databases. An importance function in a spatial database is that users can browse the geometric objects as a map efficiently. Thus the spatial database should display the geometric objects users concern about swiftly onto the display window. This process includes two operations:retrieve data from database and then draw them onto screen. Accordingly, to improve the efficiency, we should try to reduce time of both retrieving object and displaying them. The former can be achieved with the aid of spatial index such as R-tree, the latter require to simplify the objects. Simplification means that objects are shown with sufficient but not with unnecessary detail which depend on the scale of browse. So the major problem is how to retrieve data at different detail level efficiently. This paper introduces the implementation of a multi-scale index in the spatial database SISP (Spatial Information Shared Platform) which is generalized from R-tree. The difference between the generalization and the R-tree lies on two facets: One is that every node and geometric object in the generalization is assigned with a importance value which denote the importance of them, and every vertex in the objects are assigned with a importance value,too. The importance value can be use to decide which data should be retrieve from disk in a query. The other difference is that geometric objects in the generalization are divided into one or more sub-blocks, and vertexes are total ordered by their importance value. With the help of the generalized R-tree, one can easily retrieve data at different detail levels.Some experiments are performed on real-life data to evaluate the performance of solutions that separately use normal spatial index and multi-scale spatial index. The results show that the solution using multi-scale index in SISP is satisfying.     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

A computer generator for randomly layered structures

AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…     

Perspherical Approaches for Electrons in Atoms and Charged Excitons in Quantum Wells

Instead of following Fock’s expansion,we solve the Schrodinger equation for some quantum mechanical manybody systems such as electrons in atoms and charged excitons in quantum wells in a similar way in hyperspherical coordinates by expanding the wave functions into orthonormal complete basis sets of the hyperspherical hannonics(HHs)of hyperangles and generalized Laguerre polynomials(GLPs)of the hyperradius.This leads the equation to     

Advances in broadband wireless communications under high-mobility scenarios

Future mobile communication systems aim at providing very high data transmission rates, even in high-mobility scenarios such as high-speed wheel-track trains, maglev trains, highway vehicles, airplanes, guided missiles or spacecraft. A particularly important commercial application is the strong and increasing worldwide demand for high- speed broadband wireless communications （up to 574.8 km/ h test speeds or 380 km/h commercial speeds） in railways, providing data, voice and video services for applications such as onboard entertainment services to passengers, train control, train dispatch, train sensor status handling and sur- veillance. In such high-mobility scenarios, there are a number of communication challenges, including fast hand- over, location updating, high-speed channel modeling, estimation and equalization, anti-Doppler spreading tech- niques, fast power control, and dedicated network architec- ture. Because signal transmission in very high-speed scenarios will inevitably experience serious deterioration, it is imperative to develop key broadband mobile communi- cation techniques for such high-speed vehicles.     

Wuhan University Journal of Natural Sciences TOTAL CONTENTS

<正>~~     

海南岛地体及其毗邻陆缘晚中生代—新生代古地磁研究和构造演化

本文叙述了对海南岛及其毗邻大陆边缘白垩纪到第四纪地层岩石进行古地磁研究的全部工作过程。通过分析岩石中剩余磁矢量的磁偏角及磁倾角的变化,提出海南岛白垩纪以来经历的构造演化模式如下:早期伴随顺时针旋转而向南迁移,后期伴随逆时针转动并向北运移。联系该地区及邻区的地质、地球物理资料,对海南岛上述的构造地体运动提出以下认识:北部湾内早期有一拉张作用,主要是该作用使湾内地壳显著伸长减薄,形成北部湾盆地。从而导致了海南岛的早期构造运动,而海南岛后期的构造运动则主要是受南海海底扩张的影响。海南地体运动规律的阐明对于了解北部湾油气盆地的形成演化有重要的理论和实际意义。     

Scale-based spatial data model for GIS

Being the primary media of geographical information and the elementary objects manipulated, almost all of maps adopt the layer-based model to represent geographic information in the existent GIS. However, it is difficult to extend the map represented in layer-based model. Furthermore, in Web-Based GIS, It is slow to transmit the spatial data for map viewing. In this paper, for solving the questions above, we have proposed a new method for representing the spatial data. That is scale-based model. In this model we represent maps in three levels: scale-view, block, and spatial object, and organize the maps in a set of map layers, named Scale-View, which associates some given scales.Lastly, a prototype Web-Based GIS using the proposed spatial data representation is described briefly.     

Exciton Seebeck in Molecular Systems

Various applications relevant to the exciton dynamics,such as the organic solar cell,the large-area organic light-emitting diodes and the thermoelectricity,are operating under temperature gradient.The potential abnormal behavior of the exicton dynamics driven by the temperature difference may affect the efficiency and performance of the corresponding devices.In the above situations,the exciton dynamics under temperature difference is mixed with     

Achieving Unusual States of Matter Under High Pressure

The elongation method,originally proposed by Imamura was further developed for many years in our group.As a method towards O(N)with high efficiency and high accuracy for any dimensional systems.This treatment designed for one-dimensional(ID)polymers is now available for three-dimensional(3D)systems,but geometry optimization is now possible only for 1D-systems.As an approach toward post-Hartree-Fock,it was also extended to