高温胁迫对菠菜类囊体膜蛋白亚基和光谱特征的影响

研究了高温处理对菠菜类囊体膜蛋白亚基和光谱特征的影响.结果显示,高温处理后,内周天线CP43、放氧外周蛋白33 000降解明显,而外周天线LHC II发生聚集;随着处理温度的增高,菠菜类囊体膜的吸收光谱和荧光发射光谱明显下降,而且峰位发生蓝移,这说明高温处理破坏了类囊体膜的结构,影响到对光能的吸收.而35℃高温处理下,随着处理时间的延长,吸收光谱和荧光光谱也呈现下降趋势,但与不同温度处理相比,下降程度略缓,这说明类囊体膜在35℃长时间处理表现出的耐受性比在高温短时间处理条件强.     

蓝隐藻的培养及色素-蛋白复合物分离

探索了蓝隐藻的实验室自行培养条件;改进了完整类囊体膜的制备方法,获得了状态较为理想的类囊体膜,其Chla/c比值可稳定在4左右.此外,建立并优化了色素-蛋白复合物的增溶以及蔗糖密度梯度离心分离的条件,得到了4种活性的色素-蛋白复合物,经光氧化活性测定,初步判定为捕光复合物Ⅰ、捕光复合物Ⅱ以及PSⅡ颗粒和PSⅠ颗粒.实验结果为今后进一步研究蓝隐藻光合系统结构奠定了基础.     

聚球藻类囊体膜上存在两种结合类型的藻胆体的研究

采用0.05mol／L磷酸缓冲溶液处理聚球藻藻胆体-类囊体膜．使藻胆体从类囊体膜上解离出来的方法研究藻胆体在类囊体膜上结合状态研究发现并首次报导在该藻类囊体膜上存在两类结合类型的藻胆体：一类藻胆体与类囊体膜结合不紧密，很易从类囊体膜上解离出来；另一类藻胆体与类囊体膜结合很紧，不易从类囊体膜上解离出来     

光照条件下Rose bengal处理对植物类囊体膜，PS II颗粒活性及多肽组分的影响

研究了Rose bengal处理对菠菜类囊体膜及PS II颗粒的叶绿素荧光发射光谱、蛋白质内源荧光发射光谱、DCIP光还原活性及多肽组分的影响。结果表明：单线态氧可改变类囊体膜的结构,并且可破坏PS II反应中心及LHC II中叶绿素分子的结合状态,引起类囊体膜PS II天线系统中的叶绿素捕光效率下降,还可引起光合电子传递能力下降和类囊体膜蛋白构象的改变,但至少在短时间内不会造成类囊体膜多肽组分的降解。     

藻胆体与类囊体膜之间的能量传递研究进展

藻胆体是蓝藻及红藻主要的捕光天线,可以将吸收的光能传递给类囊体膜。该文首先对蓝藻及红藻藻胆体中各藻胆蛋白组分的能量传递规律,及藻胆体与类囊体膜之间能量传递机制进行了概述,认为能量传递效率与各蛋白组分的相对位置及其相互之间的光谱匹配程度有关;能量传递需要多种机制的共同参与,只是所占比例不同;状态转换不是在极端的实验条件下才发生的,而在日常条件下就可以发生。此外,还概述了藻胆体或藻胆蛋白与高等植物类囊体膜之间的能量传递,认为藻胆体或藻胆蛋白与高等植物类囊体之间能发生能量传递,不同的藻胆蛋白与高等植物类囊体之间的传递效率有所不同。最后对建立高效的植物光能传递系统的研究方向提出了展望。     

栽培大麦叶细胞的亚显微结构简报

本文用透射电镜研究了栽培大麦(Hordeum Vulgare L.)正常叶细胞的亚显微结构。结果指出:叶绿体类囊体膜系统发达,基粒类囊体垛叠层数多,基粒体积大,基质物质浓等为其共同特征。并对细胞核作了扼要介绍。     

Rose bengal处理对植物类囊体膜和PSⅡ颗粒活性的影响

研究了Rose bengal处理对菠菜类囊体膜及PSⅡ颗粒的叶绿素荧光发射光谱、蛋白质内源荧光发射光谱及DCIP光还原活性的影响. 结果表明:单线态氧可改变类囊体膜的结构,并且可破坏PSⅡ反应中心及LHCⅡ中叶绿素分子的结合状态,引起类囊体膜PSⅡ天线系统中的叶绿素捕光效率下降,还可引起光合电子传递能力下降和类囊体膜蛋白构象的改变.     

幼叶黄化油菜(Brassica napus L.)突变体Cr3529叶绿体超微结构观察

对油菜Cr3529叶片细胞中的叶绿体数目及结构进行了显微及亚显微观察。结果显示，突变与野生型细胞中叶绿体的数目没有显著性的差异，但叶绿体的结构有较大的差异，较之正常油菜，Cr3529油菜叶绿体基粒类囊体数较少，基质类囊体数较多，而且，在基粒类囊体中基粒片层数也较少，大部分基粒只有几个片层，基粒的平均片层数为5．46，约为野生型的一半，作者认为，较少的基粒片层数可能是引起Cr3529油菜叶绿素含量减少，并最终导致植株显著减产的重要原因。     

水稻类囊体膜ATP酶性质的研究

水稻类囊体膜上存在着CF_1-CF_o蛋白复合体.在光合磷酸化时,偶联因子CF_1能催化ATP的形成;在一定的条件下它又显示出了ATP酶水解活性.CF_1-CF_o蛋白复合体在植物能量代谢中起着十分重要的作用.CF_o因子嵌入类囊体膜内,起着质子通道的作用,参与质子传递,而CF_1突出在类囊体膜外,具有水解ATP的功能.我们从水稻珍汕97B叶片中分离出类囊体膜,着重研究了CF_1-ATP酶的生物化学性质. 水稻类囊体膜用差速离心法制备,取500克新鲜的水稻叶片,加入2000ml pH7.8的缓冲液(内含0.4M蔗糖、0.01MKCl和0.05M Tris-HCl),在4℃时,用捣碎机充分捣碎,然后用尼龙布过滤收集滤液,经600g离心10分钟,上清液再经6000g离心5分     

p38α半胱氨酸点突变体真核表达载体的构建和表达

以pFLAG-CMV-5.1-p38α为模板,应用PCR定点突变技术将p38α的第39、119、162和211位半胱氨酸(Cys)点突变体定点突变为丙氨酸(Ala)(C39A、C119A、C162A、C211A),使用琼脂糖凝胶电泳及基因测序检测其结果;并采用脂质体转染法将p38α的野生型和突变体质粒分别转入HEK293细胞,免疫印迹鉴定野生型及其突变体p38蛋白表达。结果显示,构建的C39ApFLAG-CMV-5.1、C119ApFLAGCMV-5.1、C162ApFLAG-CMV-5.1和C211ApFLAG-CMV-5.1重组载体成功点突变,并在HEK293细胞中高效表达,为深入研究p38在缺血性脑损伤中的作用及机制打下基础。     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

An Index mechanism for multi-scale view in spatial databases

There are numerous geometric objects stored in the spatial databases. An importance function in a spatial database is that users can browse the geometric objects as a map efficiently. Thus the spatial database should display the geometric objects users concern about swiftly onto the display window. This process includes two operations:retrieve data from database and then draw them onto screen. Accordingly, to improve the efficiency, we should try to reduce time of both retrieving object and displaying them. The former can be achieved with the aid of spatial index such as R-tree, the latter require to simplify the objects. Simplification means that objects are shown with sufficient but not with unnecessary detail which depend on the scale of browse. So the major problem is how to retrieve data at different detail level efficiently. This paper introduces the implementation of a multi-scale index in the spatial database SISP (Spatial Information Shared Platform) which is generalized from R-tree. The difference between the generalization and the R-tree lies on two facets: One is that every node and geometric object in the generalization is assigned with a importance value which denote the importance of them, and every vertex in the objects are assigned with a importance value,too. The importance value can be use to decide which data should be retrieve from disk in a query. The other difference is that geometric objects in the generalization are divided into one or more sub-blocks, and vertexes are total ordered by their importance value. With the help of the generalized R-tree, one can easily retrieve data at different detail levels.Some experiments are performed on real-life data to evaluate the performance of solutions that separately use normal spatial index and multi-scale spatial index. The results show that the solution using multi-scale index in SISP is satisfying.     

A computer generator for randomly layered structures

AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…     

海南岛地体及其毗邻陆缘晚中生代—新生代古地磁研究和构造演化

本文叙述了对海南岛及其毗邻大陆边缘白垩纪到第四纪地层岩石进行古地磁研究的全部工作过程。通过分析岩石中剩余磁矢量的磁偏角及磁倾角的变化,提出海南岛白垩纪以来经历的构造演化模式如下:早期伴随顺时针旋转而向南迁移,后期伴随逆时针转动并向北运移。联系该地区及邻区的地质、地球物理资料,对海南岛上述的构造地体运动提出以下认识:北部湾内早期有一拉张作用,主要是该作用使湾内地壳显著伸长减薄,形成北部湾盆地。从而导致了海南岛的早期构造运动,而海南岛后期的构造运动则主要是受南海海底扩张的影响。海南地体运动规律的阐明对于了解北部湾油气盆地的形成演化有重要的理论和实际意义。     

Exciton Seebeck in Molecular Systems

Various applications relevant to the exciton dynamics,such as the organic solar cell,the large-area organic light-emitting diodes and the thermoelectricity,are operating under temperature gradient.The potential abnormal behavior of the exicton dynamics driven by the temperature difference may affect the efficiency and performance of the corresponding devices.In the above situations,the exciton dynamics under temperature difference is mixed with     

Achieving Unusual States of Matter Under High Pressure

The elongation method,originally proposed by Imamura was further developed for many years in our group.As a method towards O(N)with high efficiency and high accuracy for any dimensional systems.This treatment designed for one-dimensional(ID)polymers is now available for three-dimensional(3D)systems,but geometry optimization is now possible only for 1D-systems.As an approach toward post-Hartree-Fock,it was also extended to     

《Chinese Science Bulletin》SUBJECT INDEX TO VOLUME 59(2014)

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An on-line scaling method for improving scalability of a database cluster

The explosive growth of the Internet and database applications has driven database to be more scalable and available, and able to support on-line scaling without interrupting service. To support more client's queries without downtime and degrading the response time, more nodes have to be scaled up while the database is running. This paper presents the overview of scalable and available database that satisfies the above characteristics. And we propose a novel on-line scaling method. Our method improves the existing on-line scaling method for fast response time and higher throughputs. Our proposed method reduces unnecessary network use, i.e. , we decrease the number of data copy by reusing the backup data. Also, our on-line scaling operation can be processed parallel by selecting adequate nodes as new node. Our performance study shows that our method results in significant reduction in data copy time.     

An improved R-tree based on childnode''s probability

R-Tree is a good structure for spatial searching. But in this indexing structure,either the sequence of nodes in the same level or sequence of traveling these nodes when queries are made is random. Since the possibility that the object appears in different MBR which have the same parents node is different, if we make the subnode who has the most possibility be traveled first, the time cost will be decreased in most of the cases. In some case, the possibility of a point belong to a rectangle will shows direct proportion with the size of the rectangle. But this conclusion is based on an assumption that the objects are symmetrically distributing in the area and this assumption is not always coming into existence. Now we found a more direct parameter to scale the possibility and made a little change on the structure of R-tree, to increase the possibility of founding the satisfying answer in the front sub trees. We names this structure probability based arranged R-tree (PBAR-tree).     

Integrating GIS Web services based on mediating architecture

The geographic information service is enabled by the advancements in general Web service technology and the focused efforts of the OGC in defining XML-based Web GIS service. Based on these models, this paper addresses the issue of services chaining,the process of combining or pipelining results from several interoperable GIS Web Services to create a customized solution. This paper presents a mediated chaining architecture in which a specific service takes responsibility for performing the process that describes a service chain. We designed the Spatial Information Process Language (SIPL) for dynamic modeling and describing the service chain, also a prototype of the Spatial Information Process Execution Engine (SIPEE) is implemented for executing processes written in SIPL. Discussion of measures to improve the functionality and performance of such system will be included.