蛹虫草纤溶酶液体发酵条件的优化

在发现蛹虫草具有纤溶酶活性的基础上,对蛹虫草产纤溶酶的液体发酵条件进行了优化,以期获得最佳的产酶条件。研究结果表明,蛹虫草纤溶酶的最优培养基组成是：以20 g/L可溶性淀粉为碳源,10 g/L酵母粉为氮源,Mg2＋浓度为7 mmol/L;最适发酵初始pH值为6;最适发酵时间为4 d。     

蛹虫草菌丝体液体培养条件研究

研究蛹虫草液体发酵条件,以干菌体得率为指标,通过单因素试验确定适宜的碳源、氮源、培养温度和pH值。结果表明,最适液体培养基为:葡萄糖20 g/L,蛋白胨5.0 g/L,最适pH=6.0,最适培养温度24℃。     

蛹虫草液体培养联产胞外多糖与菌丝虫草素的实验研究

通过对蛹虫草(Cordyceps militaris)液体发酵产胞外多糖和菌丝虫草素的初步实验研究,获得了蛹虫草液体发酵联产胞外多糖和菌丝虫草素的适宜参数.研究结果表明,蛹虫草液体发酵联产胞外多糖和菌丝虫草素的较适宜的氮源是豆粕粉和6%~8%玉米浆粉;较适宜的碳源是玉米面;维生素B1添加量以10 mg/100 m L、微量元素锰添加量以0.01%Mn SO4为宜、培养基初始p H值以5.5为宜;培养温度22~24℃,接种量1%,培养基装量50 m L和5~7 d的培养时间有利于胞外多糖和菌丝虫草素的产生.     

桑黄液体深层发酵培养基优化研究

以菌丝体生物量和多糖产量为主要指标,对桑黄(鲍氏层孔菌)的液体深层发酵培养基进行了优化,通过单因素试验筛选碳源、氮源,通过正交试验优化桑黄液体深层发酵的培养基。结果表明:蔗糖为最佳碳源,氯化铵为最佳氮源;最适培养基各成分的质量浓度为蔗糖70g/L、氯化铵10g/L、磷酸二氢钾0.5g/L、硫酸镁0.5g/L、氯化钠1g/L,pH值自然,以此条件发酵最大菌丝量为2.814g。在接种量5%、装液量50mL、28℃发酵过程中发现,发酵120h时桑黄菌丝体生成量最大,随后呈下降趋势;发酵液pH值随着发酵进程呈现明显下降,在120h后基本趋于稳定。     

羊肚菌(Ydj0705)液体发酵工艺方法的研究

研究羊肚菌Ydj0705的液体发酵培养基配方,确定液体发酵的工艺条件及方法,开发真菌资源菌丝产品。采用正交设计法,优化羊肚菌Ydj0705培养基配方和培养条件,从而建立羊肚菌Ydj0705液体发酵工艺。结果显示,羊肚菌Ydj0705液体发酵培养基最佳配方为:玉米粉30g/L,葡萄糖10g/L,黄豆粉20g/L,酵母粉5g/L,KH2PO4和MgSO4适量;最优液体发酵培养条件为:培养温度26℃,摇床转速定量,培养基初始pH为6.0,装液量为100mL培养液,接种量10%,发酵时间144h。羊肚菌Ydj0705液体发酵工艺能高效生产菌丝体,为开发羊肚菌真菌资源产品奠定了基础。     

蛹虫草液体发酵培养基的筛选

本文为了获得在蛹虫草液体发酵培养条件下,菌丝体干重得率和干菌丝体多糖含量综合较高的培养基.通过精选15个培养基配方进行了液体摇瓶发酵试验.结果表明:筛选出的4号作为最适培养基,其菌丝体干重得率为23.32g/L,干菌丝体多糖含量为4.27%.     

蛹虫草液体发酵条件的研究

通过单因子筛选和正交试验 ,对蛹虫草发酵条件进行研究 .试验结果表明 ,蛹虫草发酵的适宜培养基组成为 :蔗糖 5 .0 % ,玉米浆 3.0 % ,酵母膏 0 .5 % ,MgSO4 ·7H2 O 0 .0 5 % ,KH2 PO40 .0 5 % ;适宜的培养条件为 :发酵初始pH 7.0 ,发酵温度 2 5℃ ,发酵周期 6d .采用优化的培养基及适宜的培养条件进行摇瓶发酵 ,干菌体产量和生长速率分别可达 4 1.1g/L和 0 .2 8g/L·h .     

蛹虫草高产菌丝体和虫草菌素的深层培养工艺优化(英文)

蛹虫草Cordyceps militaris是当前用于功能食品和药物开发上较受国内外关注的真菌之一。本研究通过1次一因素法和正交实验设计优化了蛹虫草产虫草菌素和菌丝体的深层培养工艺。最适合菌丝生长的pH值和温度为6.0和20℃,而最适合虫草素积累的pH值和温度为5.0和26℃。蔗糖、蛋白胨、MgSO4、K2HPO4和NAA分别是最适合虫草素积累的碳源、氮源、无机盐和生长因子;培养基的成分对蛹虫草生物量的影响次序为:蔗糖>蛋白胨>MgSO4·7H2O>K2HPO4>NAA;培养基的成分对蛹虫草产虫草素的影响次序为:蛋白胨>K2HPO4>NAA>MgSO4·7H2O>蔗糖。最适合蛹虫草菌丝生长的培养基配比为4%蔗糖,2.5%蛋白胨,0.05%MgSO4·7H2O,0.15%K2HPO4,2.0mg/l NAA;最适合蛹虫草深层发酵产虫草素的培养基配比为4%蔗糖,1.5%蛋白胨,0.05%MgSO4·7H2O,0.05%K2HPO4,4.0mg/l NAA的培养基;在最佳蛹虫草深层发酵产虫草素的工艺条件下,CM8菌株胞外虫草菌素产量达到961.21 mg/l,胞内虫草菌素的含量达到12.87mg/g;菌丝体的产量为22.97 g/l,本研究得出的结论对深层培养提高蛹虫草虫草菌素和菌丝体的产量有借鉴意义。     

蚕蛹虫草菌丝生物学特性的研究

对蛹虫草菌丝体进行液体摇瓶培养，以菌丝体干重为指标，从碳源、氮源、pH、温度等方面探讨了蛹虫草菌丝的生物学特性．结果表明，蛹虫草菌丝的生长可以利用多种碳源和氮源，在以红糖为碳源、奶粉为氮源的液体培养基中生长最佳，且碳源／氮源合适的比例为2．0—2．5／1．0，适宜pH为5．0—6．5，最适pH为6．0，适宜生长温度范围为15—30℃，最适温度为25℃．     

优化条件对蛹虫草菌丝体与胞外多糖得率研究

采用摇瓶法对蛹虫草无性型菌种(ACCC 50383)菌丝体发酵培养,以菌丝体与胞外多糖得率为指标,通过单因素方差分析、S-N-K多重比较和回归方程分析,分别考察6种碳源和6种氮源,确定最适碳源为蔗糖,最适氮源为大豆蛋白胨;对发酵条件的研究表明,获得较高产率的发酵时间为6 d,最适发酵温度为(26.3±0.9)℃,发酵液初始pH值对发酵产率无影响,获得最大发酵产量时装液量为(208±28)mL。     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

A computer generator for randomly layered structures

AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…     

海南岛地体及其毗邻陆缘晚中生代—新生代古地磁研究和构造演化

本文叙述了对海南岛及其毗邻大陆边缘白垩纪到第四纪地层岩石进行古地磁研究的全部工作过程。通过分析岩石中剩余磁矢量的磁偏角及磁倾角的变化,提出海南岛白垩纪以来经历的构造演化模式如下:早期伴随顺时针旋转而向南迁移,后期伴随逆时针转动并向北运移。联系该地区及邻区的地质、地球物理资料,对海南岛上述的构造地体运动提出以下认识:北部湾内早期有一拉张作用,主要是该作用使湾内地壳显著伸长减薄,形成北部湾盆地。从而导致了海南岛的早期构造运动,而海南岛后期的构造运动则主要是受南海海底扩张的影响。海南地体运动规律的阐明对于了解北部湾油气盆地的形成演化有重要的理论和实际意义。     

Exciton Seebeck in Molecular Systems

Various applications relevant to the exciton dynamics,such as the organic solar cell,the large-area organic light-emitting diodes and the thermoelectricity,are operating under temperature gradient.The potential abnormal behavior of the exicton dynamics driven by the temperature difference may affect the efficiency and performance of the corresponding devices.In the above situations,the exciton dynamics under temperature difference is mixed with     

Achieving Unusual States of Matter Under High Pressure

The elongation method,originally proposed by Imamura was further developed for many years in our group.As a method towards O(N)with high efficiency and high accuracy for any dimensional systems.This treatment designed for one-dimensional(ID)polymers is now available for three-dimensional(3D)systems,but geometry optimization is now possible only for 1D-systems.As an approach toward post-Hartree-Fock,it was also extended to     

《Chinese Science Bulletin》SUBJECT INDEX TO VOLUME 59(2014)

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An on-line scaling method for improving scalability of a database cluster

The explosive growth of the Internet and database applications has driven database to be more scalable and available, and able to support on-line scaling without interrupting service. To support more client's queries without downtime and degrading the response time, more nodes have to be scaled up while the database is running. This paper presents the overview of scalable and available database that satisfies the above characteristics. And we propose a novel on-line scaling method. Our method improves the existing on-line scaling method for fast response time and higher throughputs. Our proposed method reduces unnecessary network use, i.e. , we decrease the number of data copy by reusing the backup data. Also, our on-line scaling operation can be processed parallel by selecting adequate nodes as new node. Our performance study shows that our method results in significant reduction in data copy time.     

An improved R-tree based on childnode''s probability

R-Tree is a good structure for spatial searching. But in this indexing structure,either the sequence of nodes in the same level or sequence of traveling these nodes when queries are made is random. Since the possibility that the object appears in different MBR which have the same parents node is different, if we make the subnode who has the most possibility be traveled first, the time cost will be decreased in most of the cases. In some case, the possibility of a point belong to a rectangle will shows direct proportion with the size of the rectangle. But this conclusion is based on an assumption that the objects are symmetrically distributing in the area and this assumption is not always coming into existence. Now we found a more direct parameter to scale the possibility and made a little change on the structure of R-tree, to increase the possibility of founding the satisfying answer in the front sub trees. We names this structure probability based arranged R-tree (PBAR-tree).     

Integrating GIS Web services based on mediating architecture

The geographic information service is enabled by the advancements in general Web service technology and the focused efforts of the OGC in defining XML-based Web GIS service. Based on these models, this paper addresses the issue of services chaining,the process of combining or pipelining results from several interoperable GIS Web Services to create a customized solution. This paper presents a mediated chaining architecture in which a specific service takes responsibility for performing the process that describes a service chain. We designed the Spatial Information Process Language (SIPL) for dynamic modeling and describing the service chain, also a prototype of the Spatial Information Process Execution Engine (SIPEE) is implemented for executing processes written in SIPL. Discussion of measures to improve the functionality and performance of such system will be included.     

Tracking data management using topology of moving object database system for moving object constrained trajectory in LBS application

Advances in wireless technologies and positioning technologies and spread of wireless devices, an interest in LBS (Location Based Service) is arising. To provide location based service, tracking data should have been stored in moving object database management system (called MODBMS) with proper policies and managed efficiently. So the methods which acquire the location information at regular time intervals then, store and manage have been studied. In this paper, we suggest tracking data management techniques using topology that is corresponding to the moving path of moving object. In our techniques, we update the MODBMS when moving object arrived at a street intersection or a curved road which is represented as the node in topology and predict the location at past and future with attribute of topology and linear function. In this technique, location data that are corresponding to the node in topology are stored, thus reduce the number of update and amount of data. Also in case predicting the location,because topology are used as well as existing location information, accuracy for prediction is increased than applying linear function or spline function.