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1.
关于渔船航行安全的探讨ONTHESAFETYOFFISHINGVESSELNAVIGATION郑奇侃,吴龙斌,朱伟ZhengQikanWuLongbingZhuWei(浙江水产学院舟山316101)(ZhejiangFisheriescollege,...  相似文献   

2.
光合细菌Chromatium vinosum可溶性氢酶小亚基基因的克隆   总被引:6,自引:2,他引:4  
光合细菌C.vinosum可溶性氢酶由52kDa和21.5kDa两个亚基组成。该酶大亚基N-末端氨基酸序列为:SRTITIEPVTRXEGHAR,小亚基N末端氨基酸序列为:STQPKITVATXLDG。根据大、小亚基N-末端氨基酸序列设计两套引物,Primer1:5’gAT(gTgAT(g/C)gTgAT(g/C)gT(g/A)Cg3’和Primer2:5’AgC AC(C/g)CAgCC(C/g  相似文献   

3.
AcNPV增强子hr5增强HBsAg基因表达的研究   总被引:3,自引:0,他引:3  
用形成包涵体(OOC+)并能利用人工合成启动序列和多角体XIV启动子表达外源基因的转移载体质粒pSXIVVI+X3将多角体基因、乙型肝炎病毒表面抗原(HBSAg)基因和苜蓿丫纹夜蛾核型多角体病毒(AcNPV)的增强子hr5部分序列同时插入无包涵体的粉纹夜蛾核型多角体病毒TnNPV-SVI-G基因组中,得到两株高效表达HBsAg基因又形成包涵体的重组病毒TnNPV-shr35-OCC+和TnNPV-shr26-OCC+.对重组病毒的酶切鉴定、DNA斑点杂交和Southernblot分析证实,外源基因及其相应的启动子和增强子序列已正确插入病毒基因组中.插入顺序中,hr5增强子是插入HBsAg基因下游,多角体基因与HBsAg基因方向相反.125Ⅰ-固相放射免疫检测和Westernblot结果表明,HBsAg基因在昆虫离体细胞中得到高效表达并保留了抗原活性.TnNPV-shr26-OCC+和TnNPV-shr35-OCC+表达的HBsA吕蛋白与没有插入增强子序列的重组病毒TnNPV—HBs85-OCC+的比较,分别提高了40%和46%.  相似文献   

4.
AUSER:APOSSIBLEVWCEPTYPEBINARYLiZongyun1)DingYuerong1)ZhangZhousheng2)LiYulan2)(1)DepartmentofAstronomy,NanjingUniversity,21...  相似文献   

5.
拟环中幂等元和半幂等元W.B.VasanthaKandasamy(DepartmentofMathematicsIndianInstituteofTechnologyMadras-600036,India)IDEMPOTENTSANDSEMI-IDE...  相似文献   

6.
APPROXIMATIONOFSINGULARINTEGRALSBYWAVELETSHuJicheng(胡继承)(WuhanUniversity,Wuhan430072)Inthispaperwearegoingtoshowhowwaveletmet...  相似文献   

7.
华木莲学名的订正   总被引:5,自引:0,他引:5  
华木莲学名的订正郑庆衍(江西宜春林科所宜春336000)关键词华木莲;落叶木莲;新改名申国分类号*718.4落叶木莲AREVISEDNAMEOFHUAMULIAN¥ZhengQingyan(YichuanForestryInstituteofJian...  相似文献   

8.
MULTIPARAMETERINVERSIONOFELASTICWAVEWITHSINGLEIMPULSESOURCEChengJianchunTaoXiupingWeiYongZhangShuyi(InstituteofAcousticsandL...  相似文献   

9.
浅谈高校办公室督促检查工作吴中平(浙江水产学院院长办公室,舟山316101)ONSUPERVISIONANDINSPECTIONWORKOFHEADMASTER-OFFICEINCOLLEGEWuZhongping(ZhEjiangFisheries...  相似文献   

10.
ONTHEEXPONENTSETOFPRIMITIVELOCALLYSEMICOMPLETEDIGRAPHS¥ZhangKeming1);BuYuehua2)(1)DepartmentofMathcmaties,NanjingUniversity,N...  相似文献   

11.
There are numerous geometric objects stored in the spatial databases. An importance function in a spatial database is that users can browse the geometric objects as a map efficiently. Thus the spatial database should display the geometric objects users concern about swiftly onto the display window. This process includes two operations:retrieve data from database and then draw them onto screen. Accordingly, to improve the efficiency, we should try to reduce time of both retrieving object and displaying them. The former can be achieved with the aid of spatial index such as R-tree, the latter require to simplify the objects. Simplification means that objects are shown with sufficient but not with unnecessary detail which depend on the scale of browse. So the major problem is how to retrieve data at different detail level efficiently. This paper introduces the implementation of a multi-scale index in the spatial database SISP (Spatial Information Shared Platform) which is generalized from R-tree. The difference between the generalization and the R-tree lies on two facets: One is that every node and geometric object in the generalization is assigned with a importance value which denote the importance of them, and every vertex in the objects are assigned with a importance value,too. The importance value can be use to decide which data should be retrieve from disk in a query. The other difference is that geometric objects in the generalization are divided into one or more sub-blocks, and vertexes are total ordered by their importance value. With the help of the generalized R-tree, one can easily retrieve data at different detail levels.Some experiments are performed on real-life data to evaluate the performance of solutions that separately use normal spatial index and multi-scale spatial index. The results show that the solution using multi-scale index in SISP is satisfying.  相似文献   

12.
Instead of following Fock’s expansion,we solve the Schrodinger equation for some quantum mechanical manybody systems such as electrons in atoms and charged excitons in quantum wells in a similar way in hyperspherical coordinates by expanding the wave functions into orthonormal complete basis sets of the hyperspherical hannonics(HHs)of hyperangles and generalized Laguerre polynomials(GLPs)of the hyperradius.This leads the equation to  相似文献   

13.
Future mobile communication systems aim at providing very high data transmission rates, even in high-mobility scenarios such as high-speed wheel-track trains, maglev trains, highway vehicles, airplanes, guided missiles or spacecraft. A particularly important commercial application is the strong and increasing worldwide demand for high- speed broadband wireless communications (up to 574.8 km/ h test speeds or 380 km/h commercial speeds) in railways, providing data, voice and video services for applications such as onboard entertainment services to passengers, train control, train dispatch, train sensor status handling and sur- veillance. In such high-mobility scenarios, there are a number of communication challenges, including fast hand- over, location updating, high-speed channel modeling, estimation and equalization, anti-Doppler spreading tech- niques, fast power control, and dedicated network architec- ture. Because signal transmission in very high-speed scenarios will inevitably experience serious deterioration, it is imperative to develop key broadband mobile communi- cation techniques for such high-speed vehicles.  相似文献   

14.
15.
Being the primary media of geographical information and the elementary objects manipulated, almost all of maps adopt the layer-based model to represent geographic information in the existent GIS. However, it is difficult to extend the map represented in layer-based model. Furthermore, in Web-Based GIS, It is slow to transmit the spatial data for map viewing. In this paper, for solving the questions above, we have proposed a new method for representing the spatial data. That is scale-based model. In this model we represent maps in three levels: scale-view, block, and spatial object, and organize the maps in a set of map layers, named Scale-View, which associates some given scales.Lastly, a prototype Web-Based GIS using the proposed spatial data representation is described briefly.  相似文献   

16.
为了有助于提高英文爱好者的个人情操、文化素养和学习兴趣,以及专业人员对英语学习和研究进行多途径的探索,本文通过一些精选诗歌的引证和分析,着重论述了喻类修辞法在英文诗歌中的运用其及效果。  相似文献   

17.
Tennessee Williams is considered as one of the most important American playwrights since World War II.The Glass Menagerie is his first successful drama,which describes a tragic situation of family and means to say that Man is unable to change the miserable life,no matter whatever means he try,This essay focuses on the analysis of the arrangement of the four main characters:Laura.Amanda,Jim and Tom to reveal the theme.Laura is fragile.Amanda is brave.Jim is vital.Tom is sensible.And all of them develop and try the different means to struggle against life,but fail tragically.With the evidence,the paper comes to conclusion naturally that Man is unable to change the miserable life,and he dooms to fail.  相似文献   

18.
The aim of this study is to investigate the diversity of Retama raetam root-nodule bacteria isolated from arid regions of Tunisia. Twelve isolates, chosen as representative for different 16S rRNA gene patterns, were characterized by 16S rRNA gene sequencing and phenotypic analysis. Isolates were assigned to Sinorhizobium, Rhizobium and Agrobacterium. Symbiotic properties of Sinorhizobium and Rhizobium isolates showed a large diversity in their capacity to infect their host plant and fix atmospheric nitrogen. Strain RK 22 identified as Rhizobium was the most effective isolate.  相似文献   

19.
正Recently,docking has been widely used to predict the binding-modes of protein-inhibitors,when the crystal complexes structure was absent.Most docking algorithms are able to generate a large number of probable conformations,it,however,is difficult to effectively evaluate these docking poses and identify the most reasonable bindingmode.In the present study,on the basis of the crystallographic data of human 3-hydroxy-3-methylglutaryl coenzyme  相似文献   

20.
介绍了WiMAX与Wi-Fi两种无线宽带接入技术,并对两者之间的关系及相互之间的影响做了对比及分析,并对WiMAX的关键技术进行了详细说明,最后对两者的联合组网方式做了简单的探讨。  相似文献   

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