蚯蚓纤溶酶与“龙心”的比较研究

以北星二号蚯蚓为材料,分离纯化蚯蚓纤溶酶。并对其理化性质、溶纤机理等与“龙心”进行了比较。蚯蚓纤溶酶和“龙心”主要组份的分子量分别为37200和43500。蚯蚓纤溶酶和“龙心”的溶纤比活力(mm~2/mg蛋白)分别为83700和24430蚯蚓纤溶酶既能直接水解血纤维蛋白,又能激活血纤溶酶原。在0—4℃时也能水解血纤维蛋白。蚯蚓纤溶酶经DEAE-Sepharose离子交换柱层析,可分离为9个纤溶活力不同的组分。     

蚯蚓类纤溶酶与“龙心”的比较研究

以人工饲养的北星二号蚯蚓(Esenia foelida)为材料,分离纯化蚯蚓类纤溶酶,并与“龙心”进行了比较,该酶可分离为9个纤溶活力、水解BAEE活力等各不相同的组分,其纤溶性质与“龙心”相似,既能直接水解血纤维蛋白,又能激活血纤溶酶原。     

以壳聚糖为基质对蚯蚓纤溶酶进行亲和层析

蚯蚓纤溶酶(Earthworm fibrinolytic enzymes)有多种同工酶,分离纯化相对比较困难.我们采用壳聚糖作为基质,大豆胰蛋白酶抑制剂为配体,戊二醛为偶联剂,分别研究了酸性和碱性条件下对蚯蚓纤溶酶的纯化效果.实验表明经壳聚糖为载体所制备的亲和吸附剂对蚓激酶具有较高的分离纯化选择性.并且在酸性条件下提取比在碱性条件下提取所得同工酶要少.     

蚯蚓纤溶酶总RNA的提取及电泳分析

分离提取纯净完整的RNA对于分子克隆实验是很重要的,而且是进行基因表达分析的基础。本实验通过总RNA纯化试剂盒(V-gene Total RNA Purification Kit)从蚯蚓体内提取蚯蚓纤溶酶总RNA,为下一步进行RT-PCR等分子克隆实验打下了基础。     

蚯蚓纤溶酶研究与应用进展

蚯蚓纤溶酶作为新一代口服溶栓药物已试用于临床。对蚯蚓纤溶酶的生化及药理性质的研究日益深入。概述了蚯蚓纤溶酶的生化特性、药理性质和临床应用研究的最新进展。     

蚯蚓纤溶酶研究进展

蚯蚓纤溶酶是蚯蚓体内含量十分丰富的一种血纤维蛋白水解酶。自1982年日本宫崎医科大学美原恒首先报道蚯蚓体内存在这种酶以来,我国兰州大学:中科院生物物理所、中科院上海生化所、渝州大学、北京大学以及清华大学、山西医学院等单位,相继对蚯蚓纤溶酶的分离纯化工艺、理化性质、生物学活性等方面都进行了广泛的研究。现就研究的进展情况,结合本人的工作介绍如下。     

蚯蚓纤溶酶研究与应用进展

蚯蚓纤溶酶作为新一代口全药物已试用于临床。对蚯蚓生化有药理性质的研究日益深椁蚯蚓纤溶酶的生化特性。药理性质和临床应用研究的最新进展。     

蚯蚓纤溶酶同工酶的分析

采用聚丙烯酰胺凝胶电泳（ＰＡＧＥ）分析了赤子爱胜蚓和秉氏环毛蚓的两个不同部位的纤溶酶同工酶酶谱。表明纤溶酶主要存在于蚯蚓的内容物中，秉氏环毛蚓内容物中的纤溶酶种类多于赤子爱胜蚓。提示用秉氏环毛蚓内容物提取蚯蚓纤溶酶，效果较好。     

大平2号蚯蚓纤溶酶酶活的测定

本文以大平2号蚯蚓为材料,制作了纤溶酶纤维蛋白水解活性测定曲线及纤维蛋白溶酶原浓度选择曲线,确定了酶活的最佳测定范围扣测定条件。测定结果表明:大平2号蚯蚓所产生的纤溶酶具有很强的纤维蛋白水解活性和纤维蛋白溶酶原激活活性,是一种很有希望的溶栓药物。     

蚯蚓纤溶酶热稳定性的研究

在37～65℃范围内对蚯蚓纤溶酶的热稳定性进行了实践研究。表明:蚯蚓纤溶酶的干燥粉末热稳定性好且耐贮藏。据实验预测其有效版为11年。     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

A computer generator for randomly layered structures

AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…     

An Index mechanism for multi-scale view in spatial databases

There are numerous geometric objects stored in the spatial databases. An importance function in a spatial database is that users can browse the geometric objects as a map efficiently. Thus the spatial database should display the geometric objects users concern about swiftly onto the display window. This process includes two operations:retrieve data from database and then draw them onto screen. Accordingly, to improve the efficiency, we should try to reduce time of both retrieving object and displaying them. The former can be achieved with the aid of spatial index such as R-tree, the latter require to simplify the objects. Simplification means that objects are shown with sufficient but not with unnecessary detail which depend on the scale of browse. So the major problem is how to retrieve data at different detail level efficiently. This paper introduces the implementation of a multi-scale index in the spatial database SISP (Spatial Information Shared Platform) which is generalized from R-tree. The difference between the generalization and the R-tree lies on two facets: One is that every node and geometric object in the generalization is assigned with a importance value which denote the importance of them, and every vertex in the objects are assigned with a importance value,too. The importance value can be use to decide which data should be retrieve from disk in a query. The other difference is that geometric objects in the generalization are divided into one or more sub-blocks, and vertexes are total ordered by their importance value. With the help of the generalized R-tree, one can easily retrieve data at different detail levels.Some experiments are performed on real-life data to evaluate the performance of solutions that separately use normal spatial index and multi-scale spatial index. The results show that the solution using multi-scale index in SISP is satisfying.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that     

On the Thermodynamic Limit for Responses to Static Electromagnetic Fields

For molecular systems,the quantum-mechanical treatment of their responses to static electromagnetic fields usually employs a scalar-potential treatment of the electric field and a vector-potential treatment of the magnetic field.Although the potential for each field separately is associated with the choice of an(unphysical)origin,the precise choice of the origin for the electrostatic field has little consequences for the results.This is different for the