两种蓝藻超低温保存抗冻保护剂的研究

以两种蓝藻为试材,对影响超低温(液氮温度-196℃)保存成活率的保护剂及其配比进行了探讨,发现非渗透保护剂(蔗糖)比渗透性保护剂(DMSO)效果好,混和保护剂与单一蔗糖为保护剂的处理效果未见区别,由两种方法所测成活率看鱼腥藻(Anabaena sp.)比钝顶螺旋藻(Spirulinaplatensis)的抗寒性高.     

不利于生长的pH条件下蓝藻液泡的形成

对鱼腥藻sp.595(Anabaenasp.595)、织线藻246(Plectonema boryanum246)、伪枝藻248(Scytonema hofmanni248)、念珠藻sp.96(Nostocsp.96)、颤藻284(Oscillatoria animlis284)和极大螺旋藻438(Spirulina maxima438)等6种蓝藻进行异常pH条件下的液泡化诱导.在pH5.0,6.5,7.0条件下,鱼腥藻sp.595和织线藻246藻细胞均出现液泡化.其中,鱼腥藻sp.595变化明显,其藻细胞膨大,两端变圆甚至球形化,液泡化程度高,且细胞不均等分裂现象普遍,织线藻246藻细胞稍有膨大,液泡化程度较轻.伪枝藻248表现较弱的敏感性,藻细胞仅在pH6.5时诱导液泡化.念珠藻sp.96、颤藻284和极大螺旋藻438在各种pH条件下均不能发生液泡化,对诱导不敏感.采用压片法检查到鱼腥藻sp.595和织线藻246诱导形成的液泡,在相差显微镜下液泡显示为圆球形,基本透明.结果说明,不利于生长的pH也能诱导蓝藻形成液泡.     

山仔水库两种水华蓝藻磷营养动力学特征

通过分析测定山仔水库两种水华蓝藻:铜绿微囊藻(Microcystis aeruginosa)、浮游鱼腥藻(Anabaena planctonica)在不同质量浓度的无机磷(K2HPO4)和有机磷(ATP)中的生长速率和生物量的变化,并利用Monod方程计算分析两种藻的磷吸收动力学参数(KS和μm),比较了两种水华蓝藻对磷的吸收差异和竞争能力.结果表明,无机磷培养条件下,动力学参数为:μm(铜绿微囊藻)μm(浮游鱼腥藻),KS(铜绿微囊藻)KS(浮游鱼腥藻).有机磷培养条件下,动力学参数较无机磷条件下要小,且两种藻有机磷条件下差异不明显.表明无机磷是水华蓝藻主要吸收的磷源,在无机磷缺乏的条件下,水华蓝藻均能吸收部分有机磷.     

植物激素对固氮鱼腥藻(Anabaena azotica ley)的生长效应实验简报

蓝藻固氮的研究在理论上和实践上都有重要意义。本工作的目的在于了解植物激素应用于固氮蓝藻培养中的价值,以便在促进水稻增产工作上有所助益。一、材料和方法实验所用藻种系取自湖北水生生物研究所的一种固氮蓝藻——固氮鱼腥藻(Anabaena azotica Ley)(水生686)。藻类培养液用水生111无氮培养液:硫酸镁(MgSO_4·7H_2O)1.25g,磷酸氢二钾(K_2HPO_4)0.75g,碳酸钙(CaCO_3)1.00g,柠檬酸铁(1%水溶液)5.00ml,柠檬     

广东固氮蓝藻肥效的研究

为促进农业大上快上、粮食丰收,广辟肥源十分重要。“红萍”(即满江红,学名Azolla inlbricata Nakai)之所以有肥效,是因为它的叶腔中有一种共生的蓝藻,即满江红鱼腥藻(Anabaena azolla Lemm.),可以固定空气中游离的氮素,并把它变为使水稻等农作物可以利用的氮化合物。我们自1973年以来,不断探索一些自生的固氮蓝藻在广东省内的培养、应用及肥效。     

铜绿微囊藻、水华鱼腥藻对不同形态磷的摄取及生理响应

研究山仔水库2种水华蓝藻:铜绿微囊藻(Microcystis aeruginosa)、水华鱼腥藻(Anabaena flosaquae)在不同形态磷(4种无机磷和2种有机磷)条件下的生长效应.通过测定胞外总磷含量及碱性磷酸酶活性,比较两者对不同形态磷的摄取能力.结果显示,2种水华蓝藻对正磷酸盐(磷酸氢二钾、磷酸二氢钾)的吸收速度和碱性磷酸酶酶活均大于多聚磷酸盐(焦磷酸钠、聚磷酸钠)和有机磷盐(ATP、甘油磷酸钠);铜绿微囊藻磷吸收速率大于鱼腥藻,但水华鱼腥藻相应的比增长速率和碱性磷酸酶酶活性均大于微囊藻,表明正磷酸盐是水华蓝藻生长所需的最适磷盐;低磷条件下,水华鱼腥藻有可能成为优势藻.     

山西忻州市温泉蓝藻及分布特点的研究

对山西忻州市的温泉蓝藻植物进行了研究．共鉴定出37种和1变种，隶属于2目，3科，14属．其中，种类最多的属为颤藻属，有7种，其次是裂面藻属和席藻属，各5种．优势种为细弱颤藻，两栖颤藻，阿氏颤藻，悦目颤藻．群落主要颤藻群落，颤藻一席藻群落，颤藻一集胞藻群落和鱼腥藻一颤藻群落。     

溶微囊藻菌的分离与溶藻作用

从太湖梅梁湾水域放置的除藻中试反应器的人工介质上分离出1株溶藻细菌,并对该株菌溶解铜绿微囊藻和降解微囊藻毒素的效果与机制进行了研究.结果表明,结合形态学、生理与生化特性以及16S rRNA特异性引物扩增综合分析,初步鉴定该株细菌属于假单胞菌属;对源自太湖的微囊藻的最低溶藻细菌浓度为105个/mL;在太湖水、PBS缓冲液和BG11微囊藻培养基等反应体系中对微囊藻均有较强的溶解作用,24 h藻细胞溶解率分别为85.9%、67.9%和91.0%,完全溶藻时间为48 h.其溶藻方式可能为分泌某种胞外物质所致.该株菌对微囊藻毒素LR(MC-LR)也具有较强的降解作用.在MC-LR起始质量浓度为2.642 μg/L时,细菌对MC-LR作用18,36和72 h的降解率分别为14.2%、51.3%和100.0%.此菌株在太湖水中保持着较好的生物活性,表现出较强的溶藻与降解MC-LR作用.     

溶藻细菌(B5)的溶藻效果与溶藻特性的初步研究

从富营养化湖泊分离获得的1株菌(B₅),具有明显的溶藻效果。通过凝聚、细胞裂解和生物降解作用能有效去除鱼腥藻细胞。B₅菌可能是通过直接接触导致藻细胞的凝集及进一步的生物降解,同时存在一种具有热稳定性且能抑制藻类细胞生长的胞外分泌物。     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

A computer generator for randomly layered structures

AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

海南岛地体及其毗邻陆缘晚中生代—新生代古地磁研究和构造演化

本文叙述了对海南岛及其毗邻大陆边缘白垩纪到第四纪地层岩石进行古地磁研究的全部工作过程。通过分析岩石中剩余磁矢量的磁偏角及磁倾角的变化,提出海南岛白垩纪以来经历的构造演化模式如下:早期伴随顺时针旋转而向南迁移,后期伴随逆时针转动并向北运移。联系该地区及邻区的地质、地球物理资料,对海南岛上述的构造地体运动提出以下认识:北部湾内早期有一拉张作用,主要是该作用使湾内地壳显著伸长减薄,形成北部湾盆地。从而导致了海南岛的早期构造运动,而海南岛后期的构造运动则主要是受南海海底扩张的影响。海南地体运动规律的阐明对于了解北部湾油气盆地的形成演化有重要的理论和实际意义。     

An Index mechanism for multi-scale view in spatial databases

There are numerous geometric objects stored in the spatial databases. An importance function in a spatial database is that users can browse the geometric objects as a map efficiently. Thus the spatial database should display the geometric objects users concern about swiftly onto the display window. This process includes two operations:retrieve data from database and then draw them onto screen. Accordingly, to improve the efficiency, we should try to reduce time of both retrieving object and displaying them. The former can be achieved with the aid of spatial index such as R-tree, the latter require to simplify the objects. Simplification means that objects are shown with sufficient but not with unnecessary detail which depend on the scale of browse. So the major problem is how to retrieve data at different detail level efficiently. This paper introduces the implementation of a multi-scale index in the spatial database SISP (Spatial Information Shared Platform) which is generalized from R-tree. The difference between the generalization and the R-tree lies on two facets: One is that every node and geometric object in the generalization is assigned with a importance value which denote the importance of them, and every vertex in the objects are assigned with a importance value,too. The importance value can be use to decide which data should be retrieve from disk in a query. The other difference is that geometric objects in the generalization are divided into one or more sub-blocks, and vertexes are total ordered by their importance value. With the help of the generalized R-tree, one can easily retrieve data at different detail levels.Some experiments are performed on real-life data to evaluate the performance of solutions that separately use normal spatial index and multi-scale spatial index. The results show that the solution using multi-scale index in SISP is satisfying.     

《Chinese Science Bulletin》SUBJECT INDEX TO VOLUME 59(2014)

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Achieving Unusual States of Matter Under High Pressure

The elongation method,originally proposed by Imamura was further developed for many years in our group.As a method towards O(N)with high efficiency and high accuracy for any dimensional systems.This treatment designed for one-dimensional(ID)polymers is now available for three-dimensional(3D)systems,but geometry optimization is now possible only for 1D-systems.As an approach toward post-Hartree-Fock,it was also extended to