Effects of lanthanum and gadolinium on proliferation and differentiation of primary osteoblasts

The effects of La³⁺ and Gd³⁺ on the proliferation, differentiation and adipogenic transdifferentiation of rat calvarial osteoblast-like cells (ROB cells) were evaluated by MTT method， measuring the activity of alkaline phosphatase (ALP) and Oil red O measurement. Both of La³⁺ and Gd³⁺ inhibited the proliferation of ROB cells at all concentrations (1×10^-5, 1×10^-6, 1×10^-7, 1×10^-8, 1×10^-9 mol?L^-1). La³⁺ at concentration of 1×10^-5 mol?L^-1 significantly increased the alkaline phosphatase activity of ROB cells up to 3 folds (P<0.01). However, the effects reversed to inhibit at other concentrations. Gd³⁺ played a negative role in the alkaline phosphatase activity. La³⁺ inhibited the adipogenic transdifferentiation of ROB cells at all concentrations in a dose-dependent way. However, Gd³⁺ promoted the adipogenic transdifferentiation of ROB cells at 1×10^-8 and 1×10^-9 mol?L^-1. These findings suggested that the effects of rare earth elements on the proliferation, differentiation and adipogenic transdifferentiation of ROB cells were dependent on their concentrations and species     

Stereoselectivity of Acetalization and Ketalization Reactions of Glycerol and Its Modified Derivatives

ＳｔｅｒｅｏｓｅｌｅｃｔｉｖｉｔｙｏｆＡｃｅｔａｌｉｚａｔｉｏｎａｎｄＫｅｔａｌｉｚａｔｉｏｎＲｅａｃｔｉｏｎｓｏｆＧｌｙｃｅｒｏｌａｎｄＩｔｓＭｏｄｉｆｉｅｄＤｅｒｉｖａｔｉｖｅｓＷａｎｇＫｅｙａｎ（汪克炎），ＹａｎｇＺｅｎｇｊｉａ（杨增家），Ｗａ...     

Simulation and evaluation of the quality and availability of typical GLI ocean image

ADEOS-II satellite will be launched in the near future. It collocates many remote sensing instruments in the same platform. Among them, Global Image (GLI) is considered to be a main sensor which will play a key role. In order to understand the characteristics of future GLI ocean color images, a simulation and evaluation of the quality and availability of GLI typical ocean image has been done. In the paper, we first introduce the simulation models briefly, and simulate typical cases of radiance images at visible channels, in which the radiance distribution is based on geographic location, the satellite orbital parameters and sensor properties. A method, complex signal noise ratio (CSNR) to evaluate the image quality and availability, is developed according to the characteristics of image. Meanwhile, a series of CSNR images are generated from the simulated radiance components for different cases, which can be used to evaluate the quality and availability of GLI images before the ADEOS-II is placed in orbit. Finally, the quality and availability of GLI images are quantitatively analyzed by the simulated CSNR data. The results will be beneficial to the people who are in charge of GLI mission or plan to use the data from GLI.     

Structures and Properties of Composite Yarns of Filaments and Wool Staple Fibers

More and more composite yarns of filament and wool staple fiber have been applied to develop the new type of fabrics. However, the great difference in yarn structures between the composite yarns and conventional staple yarns does affect the properties of yarns and fabrics. In this paper, it has been studied that the differences of the composite yarn properties of tensile, bending rigidity, torsional rigidity, the hairiness and irregularity etc.     

Measurements and analysis of force and moment of caudal fin model in C-start

The unsteady hydrodynamic forces and moments acting on caudal-fin models of fish with different shapes and different swing durations were experimentally measured to simulate the fish C-starts. The motion of models was characterized by rotating the model to a maximum deflection angle in an excursion time T_u and back to the initial position in a return time T_d around its root-axis. Studies show that the caudal-fin plays an important role in fish C-starts and the caudal-fins with different shapes and different swing durations generate different forces and moments. In addition, the hydrodynamic forces and moments acting on the models with different shapes can be normalized by the 2nd and 3rd moments of area, respectively. The forces and moments acting on the models with different swing durations, but the same ratio of T_u to Td can also be scaled.     

Function and interaction of maturation-promoting factor and mitogen-activated protein kinase during meiotic maturation and fertilization of oocyte

Mitogen-activated protein kinase (MAP kinase) cascade and maturation-promoting factor (MPF) play very important roles during meiotic maturation and fertilization of oocyte. Interaction between MAP kinase and MPF influences meiotic maturation and fertilization of oocyte throughout the animal kingdom, including stimulation of germinal vesicle breakdown (GVBD), suppression of DNA replication, control of meiotic chromosome segregation, maintenance of metaphase II arrest, and resumption and completion of second meiosis. This review focuses on the function and interaction of MAP kinase and MPF during meiotic maturation and fertilization of oocyte.     

Effects of lanthanum and gadolinium on proliferation and differentiation of primary osteoblasts

The effects of La3 and Gd3 on the proliferation, differentiation and adipogenic transdifferentiation of rat calvarial osteoblast-like cells (ROB cells) were evaluated by MTT method, measuring the activity of alkaline phosphatase (ALP) and Oil red O measurement. Both of La3 and Gd3 inhibited the proliferation of ROB cells at all concentrations (1×10-5, 1×10-6, 1×10-7, 1×10-8, 1×10-9 mol·L-1). La3 at concentration of 1×10-5mol·L-1 significantly increased the alkaline phosphatase activity of ROB cells up to 3 folds (P<0.01). However, the effects reversed to inhibit at other concentrations. Gd played a negative role in the alkaline phosphatase activity. La3 inhibited the adipogenic transdifferentiation of ROB cells at all concentrations in a dose-dependent way. However, Gd promoted the adipogenic transdifferentiation of ROB cells at 1×10 and 1×10 mol·L . These findings suggested that the effects of rare earth elements on the proliferation, differentiation and adipogenic transdifferentiation of ROB cells were dependent on their concentrations and species.     

Introduction of Department of Biological Sciences and Biotechnology

The Departm ent of Biology of Tsinghua University wasfounded in 192 6 ,discontinued from 195 2 to 1983 due to amajor restructuring of universities nationwide. It was re-established in 1984and renam ed as the Department ofBiological Sciences and Biotechnology (DBSB ) . Manyrenowned biologists,including more than 30 members of theChinese Academ y of Sciences (CAS) ,have studied orworked in this departm ent.The DBSB has m ade remarkable achievements since1984and has becom e one of the co…     

EPR and UV—vis studies of biomimetic complexes of molybdoenzyme and tungstoenzyme

The biomimetic synthesis and the study of the ca-talysis mechanism of molybdenum and tungsten oxotransfer enzyme have been attracted considerable at-tention for a long time, because the oxotransfer enzymes play an important part in the nitrogen, sulfur and carbon cycles in the biological system[1—4]. With the development of the X-ray crystallography, it is possible to determine the structure of molybdenum and tungsten enzymes. However, due to the complexity and dedicated nature of the protein…     

Synthesis and Spectrum Characteristic of the Inclusion Complex of β-CD and Pyridine-CuI

The β-Cyclodextrin consists of seven D-glucopyranoside units, which are linked by α-1, 4-glycosidic linkages. The shape of CD can be presented as a truncated cone. Its inner is hydrophobic and exterior is hydrophilic for the aggregation of the hydroxyl groups, which results in cyclodextrin to have the ability of inclusion different compounds.     

Analysis and Understanding of Edie

I．IntroductionEdieisthemaincharacterinthestory‘Snowed－Up：AMistletoeStory’．RichardJef－fries，theauthor，toldastory，intheformofadi－ary，ofayounggirlof１９andothermen，LordBil－berton，Mr．AldermanThriggandLieut．PhilipAurelles．ThethreemenalllovedEdieandwantedtomarryher，butEdielovedonlyPhilip．Herlovewasagainstherfatherswill．Theconflictwentonunderthesettingofasuddenbutcontinuoussnow－fall．Whenthesnowfallended，sodidthestory．Whatsinterestinginthestoryisthat，afterspend－ings…     

Selection and Verification of the Structural Pattern of the Penstock and Spiral Case of the TGP Power Station

1Patternselectionandver ificationoftheTGPpen stock　TheYangtzeRiverWaterResourcesCommission (CWRC)submittedASub jectReportonthePatternSelectionofthePenstockinthePowerStationoftheThreeGorgesProjectinDecember,1 993,inwhichthearrangementofthepenstockswasdefinitelystated ,twotypesofstructures (internalhydraulicpressurebornesolelybypenstockandinternalhydraulicpressurejointlybornebysteellinerandreinforcedconcrete)proposed ,acomparisonbetweenthetwooptionscomprehensivelyperformed ,andsuggesti…     

Method of AHP and Improvement of Its Uniformity Examination

该文对ＡＨＰ中的一致性检验方法进行了改进，进一步丰富了ＡＨＰ方法的内容．     

Identification of Wool and Cashmere

The quality number66 wool and the first grade cashmerewere tested and analyzed by using the scanning electronmicroscope(SEM)and the probability distribution of thediameter,the surface scale height and the surface scalethickness of the wool and the cashmere were got,thenthe error identification probability(EIP)of the three pa-rameters were calculated.After verifying that these threeparameters were linear non-related,by using the prob-ability theory,the EIPs of these three parameters weremultiplied,thus,the total EIPs of the wool and the cash-mere were reduced.The result of the experiment verifiedthis conclusion.     

Preface of The 2014 International Workshop on Frontiers of Theoretical and Computational Physics and Chemistry

The 2014 International Workshop on Frontiers of Theoretical and Computational Physics and Chemistry(WFTCPC’14)was held in Quzhou,China,on August 21-24,2014,hosted and coordinated by Professor Feng Long Gu of South China Normal University,Professor Ruiqin Zhang of City University of Hong Kong,and Professor Guoqiang Wu of Quzhou University.     

Aims and Scope of the Journal

<正>The journal of Progress in Natural.Science:Materials International sponsored and organized by both Chinese Materials Research Society(C-MRS)and International Union of Materials Research Societies(IUMRS)started to publish its first issue in November.2010.The Progress in Natural Science:Materials International provides scientists and engineers throughout the world with a central     

Ring-opening and Elimination Cooligomerization of Cyclic Carbonates and e-Caprolactone

Cyclic carbonates e.g. ethylene- and propylene carbonate undergo ring-opening polymerization （ROP） at elevated temperatures （ 〉 100 ℃ ） in the presence of initiators such as Lewis acids, bases, or transesterification catalysts to yield poly（ether carbonates） as outlined below. See Fig. 1.