高迁移组染色体蛋白-17的分离提取及其抗体ELISA测定方法的建立

纯化高迁移组染色体蛋白－１７（ＨＭＧ－１７）,建立检测抗ＨＭＧ－１７自身抗体的ＥＬＩＳＡ方法,探讨抗ＨＭＧ－１７抗体与临床疾病的关系。用水煮沸法提取,甲酸－吡啶缓冲液酸化和ＳｅｐｈａｄｅｘＧ－１５０凝胶过滤技术,自小牛胸腺组织中分离ＨＭＧ－１７,用此纯化物包被,方阵滴定,建立测定抗ＨＭＧ－１７抗体的间接ＥＬＩＳＡ方法,并进行方法学考核和临床标本检测。纯化的ＨＭＧ－１７抗原经十二烷基硫酸钠－聚丙烯酰胺凝胶电泳（ＳＤＳ－ＰＡＧＥ）分析,呈现一条分子量约９２００的蛋白带;建立的ＥＬＩＳＡ方法批内平均变异系数（ＣＶ）为５．８％,批间ＣＶ值为９．６％,ＥＬＩＳＡ抑制试验最高抑制率达８７．２％;临床检测结果表明,在系统性红斑狼疮（ＳＬＥ）、混合性结缔组织病（ＭＣＴＤ）和干燥综合征（ＳＳ）患者中分别有１７．４％～３７．３％的抗ＨＭＧ－１７抗体的阳性检出率（Ｐ＜０．０１或Ｐ＜０．０５）,而在其他自身免疫病和内科疾病者中,抗ＨＭＧ－１７抗体均为低水平。抗ＨＭＧ－１７抗体ＥＬＩＳＡ测定法具有较好的特异性和重复性,便于常规检测,该类抗体的存在似与某些自身免疫病（尤其是ＳＬＥ）的关系较为密切     

大肠杆菌中心人钠素基因在PL和T7启动子控制下的…

将Ｐ１噬菌体的ｒｅｆ基因与人心钠素的嵌合基因ＲＥＦ－ＡＮＰ分别克隆入带ＰＬ启动子和Ｔ７启动子的两种表达载体，获得高铲表达重组质粒ｐＺＪＭ１和ｐＺＪＭ４．ｐＺＪＭ１转化ＤＨ５α，ｐＺＪＭ４转化ＨＭＳ１７４，在表达细菌培养到ＯＤ６００＝０．２５时分别进行温度诱导和化学诱导，快速凝胶扫描结果表明ＲＥＰ－ＡＮＰ表达量各占细菌可溶性总蛋白的６７．１％和２８．１％；在ＯＤ６００＝１．０或２．０时进行诱导，均能     

食用油烟对大鼠体内脂质过氧化反应的影响

本研究采用动式染毒技术，将大鼠分成４个浓度组吸入豆油油烟（油温２２０℃），观察血中ＳＯＤ，ＧＳＨ，ＭＤＡ含量，肺组织中ＬＤＨ，ＡＣＰ，ＭＤＡ和肺巨噬细胞（ＰＡＭ）膜脂流动性（ＬＦＵ）等指标的变化，探讨吸入油烟的急性毒作用机理。实验结果表明：油烟平均浓度为１．０５ｍｇ／ｍ＾３时，血浆及肺组织中ＭＤＡ开始增加（Ｐ〈０．０５），且含量随吸入油烟量增加；平均浓度为９．５８ｍｇ／ｍ＾３时，全血ＳＯＤ活力明显     

黑白花乳牛关节滑膜炎的研究Ⅰ．两性霉素B诱发急性浆液性腕关节滑膜炎的实验研究

对７头一岁龄健康黑白花乳牛的１４个腕关节内注射两性霉素Ｂ（１２．５ｍｇ／关节），成功诱发急性浆液性腕关节滑膜炎（ＡＳＣＳ）．注射前及诱发关节炎后（注射后４８ｈ）分别抽取正常及炎性腕关节滑液（ＳＦ）样品，同时采集血液样品待检，检测结果表明：乳牛ＡＳＣＳ时，ＳＦ中细胞总数（ＴＣＣ）、总蛋白（ＴＰ）、乳酸脱氢酶（ＬＤＨ）、碱性磷酸酶（ＡＬＰ）、γ－谷氨酰转肽酶（γ－ＧＴ）、蛋白及ＬＤＨ同功酶各组分含量均显著增加（Ｐ＜０．０１）；蛋白及ＬＤＨ同功酶各组分百分含量（％）显著改变：白蛋白（Ａ）显著降低（Ｐ＜０．０１），α、β、γ球蛋白均显著升高（Ｐ＜０．０１），Ａ：Ｇ值（白／球比）显著下降（Ｐ＜０．０１），ＬＤＨ１显著下降（Ｐ＜０．０１），ＬＤＨ２、ＬＤＨ３、ＬＤＨ４、ＬＤＨ５增加，但差异不显著（Ｐ＞０．０５），以上各成分的对应血清值均无显著变化（Ｐ＞０．０５）．关节滑液（ＳＦ）的检测为乳牛关节炎症提供了准确诊断方法和依据，并可揭示关节在病理状态下的代谢状况，具有重要的临床指导意义．     

低特征信号NEPE推进剂配方设计及燃烧性能

根据最小自由能方法计算了铝粉含量变化对ＮＥＰＥ，ＣＭＤＢ以及固体含量为８８％的复合团体推进剂能量特性的影响．铝粉含量为５％～１８％时，ＮＥＰＥ推进剂密度比冲分别比ＣＭＤＢ和复合团体推进剂增加１１．１～１４．６ｓ·ｇ／ｃｍ３和１４．２～１８．０ｓ·ｇ／ｃｍ３．通过对低特征信号ＮＥＰＥ推进剂配方燃烧性能的研究，观察到当Ａ１含量为３％～５％时，采用３．５％的燃速催化剂仅可使压力指数降至０．６０．相应高能ＮＥＰＥ（铝粉含量等于１８％）推进剂的压力指数可降至０．５６．而降低ＡＰ粒度和增加其含量是提高燃速和降低压力指数的有效措施之一．此外，还测定了ＮＥＰＥ推进剂的燃速温度敏感系数，并与ＣＭＤＢ和复合团体推进剂数据进行了对比．     

桃实生根系不同部位若干代谢指标的比较

桃实生根系不同部位若干代谢指标的比较于凤鸣，张立彬，刘玉艳，周艳芹（河北农业技术师范学院园艺系，昌黎，０６６６００）ＣＯＭＰＡＲ１５ＯＮＢＥＴＷＥＥＮＳＯＭＥＭＥＴＡＢＯＬＩＣＩＮＤＥＸＥＳＯＦＤＩＦＦＥＲＥＮＴＰＡＲＴＳＯＦＰＥＡＣＨＳＥＥＤＩＮＧ...     

大肠杆菌中人心钠素基因在P_L和T7启动子控制下的高效表达

将Ｐ１噬菌体的ｒｅｆ基因与人心钠素的嵌合基因ＲＥＦ－ＡＮＰ分别克隆入带Ｐ＿Ｌ启动子和Ｔ７启动子的两种表达载体，获得高效表达重组质粒ｐＺＪＭ１和ｐＺＪＭ４．ｐＺＪＭ１转化ＤＨ５α、ｐＺＪＭ４转化ＨＭＳ１７４，在表达细菌培养到ＯＤ＿（６００）＝０．２５时分别进行温度诱导和化学诱导，快速凝胶扫描结果表明ＲＥＰ－ＡＮＰ表达量各占细菌可溶性总蛋白的６７．１％和２８．１％；在ＯＤ＿（６００）＝１．０或２．０时进行诱导，均能维持高效表达；且温度诱导的表达效率总是优于化学诱导。     

微分脉冲极谱法测定EMD中,电解液MnSO4—H2SOR中微量Pb及Pb—A…

在ＥＭＤ中及电解液ＭｎＳＯ４－Ｈ２ＳＯ４中的微量Ｐｂ已用微分脉冲极谱法测定，此微量Ｐｂ来源于在制备ＥＭＤ过程中，新型Ｐｂ－Ａｇ－Ｃａ合金阳极的极微溶解。将ＫＣＬ分别加入用ＫＣＬ溶解ＥＭＤ所得的Ｍｎｌｅ２中及电解液ＭｎＳＯ４－Ｈ２ＳＯ４中，作为底液，分别测定其Ｄ．Ｐ．Ｐ图，峰形良好。     

乙烯—1—辛烯共聚物熔融接枝马来酸酐的研究

以过氧化二异丙苯（ＤＣＰ）为引发剂,在双螺杆挤出机上实现了乙烯１－辛然共聚物（ＰＯＥ）与马来酸酐（ＭＡＨ）的熔融接枝反应,实验表明,ＰＯＥ熔融接枝ＭＡＨ的反应过程中伴随着交联副反应的发生;当ＭＡＨ／ＤＣＰ≥２０时,体系中不产生凝胶,随着ＭＡＨ／ＤＣＰ比值的减小（ＤＣ用量的增加）凝胶含量增加,红外光谱的测试结果进一步证实了ＰＯＥ－ｇ－ＭＡＨ大分子的存在,此外,对ＰＯＥ熔融接枝ＭＡＨ过程的机理进行了初     

高密度聚乙烯环境应力开裂机理的研究

应用ＦＴＩＲ，ＤＳＣ，ＤＭＡ技术，从高聚物具有结构多重性和形变不均一性出发，定性地探讨了ＨＤＰＥ的ＥＳＣ开裂机理。在应力作用下ＨＤＰＥ的形变不均一性所形成的微裂纹，就成为具有各自不同利害程度的自然内存缺陷，从而活性介质获得了渗透到ＨＤＰＥ内部的机会，使ＨＤＰＥ表面能下降，导致应力集中链的滑脱和解缠，当滑脱和解缠的分子链积累到某临界值时，便呈现了宏观脆性开裂。并根据ＨＤＰＥ的ＥＳＣ开裂机理，提出了改善ＨＤＰＥ的ＥＳＣＲ性能的途径。ＨＤＰＥ的ＥＳＣＲ性能的提高取决于对其影响因素的最佳利用。     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

A computer generator for randomly layered structures

AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

海南岛地体及其毗邻陆缘晚中生代—新生代古地磁研究和构造演化

本文叙述了对海南岛及其毗邻大陆边缘白垩纪到第四纪地层岩石进行古地磁研究的全部工作过程。通过分析岩石中剩余磁矢量的磁偏角及磁倾角的变化,提出海南岛白垩纪以来经历的构造演化模式如下:早期伴随顺时针旋转而向南迁移,后期伴随逆时针转动并向北运移。联系该地区及邻区的地质、地球物理资料,对海南岛上述的构造地体运动提出以下认识:北部湾内早期有一拉张作用,主要是该作用使湾内地壳显著伸长减薄,形成北部湾盆地。从而导致了海南岛的早期构造运动,而海南岛后期的构造运动则主要是受南海海底扩张的影响。海南地体运动规律的阐明对于了解北部湾油气盆地的形成演化有重要的理论和实际意义。     

An Index mechanism for multi-scale view in spatial databases

There are numerous geometric objects stored in the spatial databases. An importance function in a spatial database is that users can browse the geometric objects as a map efficiently. Thus the spatial database should display the geometric objects users concern about swiftly onto the display window. This process includes two operations:retrieve data from database and then draw them onto screen. Accordingly, to improve the efficiency, we should try to reduce time of both retrieving object and displaying them. The former can be achieved with the aid of spatial index such as R-tree, the latter require to simplify the objects. Simplification means that objects are shown with sufficient but not with unnecessary detail which depend on the scale of browse. So the major problem is how to retrieve data at different detail level efficiently. This paper introduces the implementation of a multi-scale index in the spatial database SISP (Spatial Information Shared Platform) which is generalized from R-tree. The difference between the generalization and the R-tree lies on two facets: One is that every node and geometric object in the generalization is assigned with a importance value which denote the importance of them, and every vertex in the objects are assigned with a importance value,too. The importance value can be use to decide which data should be retrieve from disk in a query. The other difference is that geometric objects in the generalization are divided into one or more sub-blocks, and vertexes are total ordered by their importance value. With the help of the generalized R-tree, one can easily retrieve data at different detail levels.Some experiments are performed on real-life data to evaluate the performance of solutions that separately use normal spatial index and multi-scale spatial index. The results show that the solution using multi-scale index in SISP is satisfying.     

《Chinese Science Bulletin》SUBJECT INDEX TO VOLUME 59(2014)

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Achieving Unusual States of Matter Under High Pressure

The elongation method,originally proposed by Imamura was further developed for many years in our group.As a method towards O(N)with high efficiency and high accuracy for any dimensional systems.This treatment designed for one-dimensional(ID)polymers is now available for three-dimensional(3D)systems,but geometry optimization is now possible only for 1D-systems.As an approach toward post-Hartree-Fock,it was also extended to