Ⅰ型鸭肝炎病毒(MY株)活疫苗毒种的纯粹及生产条件探索

将Ⅰ型鸭肝炎鸡胚化弱毒MY株进行纯粹检验；以100ELD50 0.2mL/枚(含病毒3.0×105个拷贝)剂量经鸡胚尿囊腔接种10日龄SPF鸡胚,每隔12h观察并采集鸡胚胚体和尿囊液样本,运用自行建立的检测DHV-1的SYBR Green I实时荧光定量RT-PCR方法检测MY株弱毒的鸡胚增殖规律.结果表明,该毒种符合...     

鸡蛋、鸡胚的SOD和MDA水平

实验对鸡蛋蛋黄、鸡蛋蛋清、鸡胚尿囊液和鸡胚羊水中的SOD和MDA进行了测定.结果表明,蛋黄、蛋清、鸡胚尿囊液都含有SOD,含量分别为46.8892±8.2668nmol/mL、31.4759±4.2049nmol/mL和40.6051±11.8457nmol/mL;鸡蛋蛋黄、鸡蛋蛋清都含有MDA,含量分别为24.8790±2.1320nmol/mL和1.5694±0.6507nmol/mL,蛋黄含量为蛋清的20倍左右.本实验结果在理论上对鸡蛋和鸡胚(毛蛋)的养生、保健和治病作用是有力的支持.     

鸡胚复方制剂对小鼠游泳时间及脾脏指数的影响

实验以45～60d龄小白鼠为研究对象,用鸡胚尿囊液、枸杞、维生素C配制成的鸡胚复方制剂灌胃10d后,测定游泳时间和脾脏指数.结果表明,鸡胚尿囊液配合枸杞或维生素C后可极显著(P<0.01)或显著(P<0.05)提高小白鼠的脾脏指数;鸡胚尿囊液配合枸杞和维生素C可极显著(P<0.01)延长小白鼠的游泳时间.     

鸡胚对小鼠抗衰老和抗疲劳的作用

以小白鼠为研究对象 ,分别进行了鸡胚在小鼠抗衰老和抗疲劳方面作用的实验 .实验结果证明 ,用 10d龄鸡胚物质灌服小鼠 4 2d后 ,小鼠血浆SOD含量明显升高 ,浆MDA水平显著降低 (P <0 .0 1) ,从而证明鸡胚对小鼠有显著的抗衰老作用 .游泳实验证明 ,用 10d龄鸡胚物质给小鼠灌服 10d后 ,游泳持续时间较对照组显著提高 (P <0 .0 1) .     

鸡胚复方制剂对小白鼠生长、脾脏指数及血浆SOD和MDA的影响

实验以不同配比的鸡胚尿囊液、枸杞和维生素C制成的鸡胚复方制剂对小白鼠进行了30d的灌胃,研究了鸡胚复方制剂对小白鼠生长、脾脏指数以及血浆SOD和MDA水平的影响.结果表明,不同配方的鸡胚复方制剂对小白鼠的生长、脾脏指数和血浆SOD和MDA水平均无明显的影响(P>0 05).同时表明鸡胚尿囊液、枸杞和维生素C在以促生长、提高免疫力和抗衰老为目的配合应用是不宜的,它们之间有拮抗作用.     

长爪沙鼠仙台病毒( SeV) 抗体ELISA 检测方法的建立与应用

摘要: 目的建立长爪沙鼠仙台病毒( SeV) 抗体ELISA 检测方法,用于长爪沙鼠体内仙台病毒抗体的检测。方法孵育鸡胚,接种SeV,制备鸡胚尿囊液正常抗原和SeV 特异抗原,滴定酶结合物和抗原最佳工作浓度,并进行特异性、敏感性、精密性、稳定性实验。结果正常抗原、特异抗原和酶结合物最佳工作浓度分别为0. 1 μg /mL、2 μg /mL 和1∶ 5 000; 正常抗原、特异抗原批内变异系数分别为9. 6% 和8. 4%,批间平均变异系数分别为9. 0% 和5. 9%; 检测灵敏度为1∶ 10 240; 与沙鼠小鼠肝炎病毒( MHV) 、小鼠肺炎( PVM) 、呼肠孤病毒3 型( Reo3) 均无交叉反应。稳定性试验相对偏差小于25%。结论建立的ELISA 方法特异性、敏感性强,重复性、稳定性好,检测结果,准确、可靠。可用于长爪沙鼠体内SeV 抗体的检测。     

乙型流感病毒在不同胚龄鸡胚中适宜孵育条件的研究

为了找出乙型流感病毒毒株在不同胚龄鸡胚中适宜孵育条件,提升血凝滴度,从而为产业化生产人用3价流感疫苗提高产能,主要采用血凝法检定收获尿囊液,分析试验血凝滴度数据,得出结论:乙型流感病毒毒株接种于10 d胚龄鸡胚,其孵育温度为35℃,孵育时间72 h最理想;孵育温度和时间对乙型流感病毒的血凝滴度有影响,同时鸡胚胚龄与其的血凝滴度和收获尿囊液量也有明显的关联。     

川芎嗪与黄芪甲苷不同浓度配伍对血管新生的影响

研究川芎嗪与黄芪甲苷不同配伍浓度对鸡胚绒毛尿囊膜模型(chick embryochorioallantoic membrane,CAM)血管新生的作用,探讨二药配伍对缺血性心脏病的影响.将100只8 d龄鸡胚随机分为10组,包括空白DMSO对照组、不同浓度的川芎嗪(1、2、4 mg/m L)与黄芪甲苷(0.2、0.4、0.8 mg/m L)两两配伍组共9组,每组10只鸡胚.采用鸡胚绒毛尿囊膜(CAM)法,通过混合纤维素微孔滤膜载体将药物加到CAM表面上,继续孵化3 d观察血管生长状况,并进行新生血管计数.结果表明,川芎嗪与黄芪甲苷各浓度配伍均能促进鸡胚绒毛尿囊膜血管新生,且与浓度有一定的剂量依赖关系.其中,川芎嗪2 mg/m L+黄芪甲苷0.4 mg/m L一级血管与二级血管管与空白组比较,有明显差异(P0.001,P0.001).川芎嗪黄芪甲苷配伍可促进鸡胚绒毛脲囊膜血管新生为二药配伍治疗缺血性心脏病提供了理论依据.     

胸腺素α_1对鸡生长性能及免疫器官发育的影响

通过对照试验在 4日龄和 7日龄时 ,低剂量组、中剂量组和高剂量组每只鸡分别肌注 0 .0 1 5 mg·kg- 1 ,0 .0 3 mg· kg- 1 和 0 .0 6mg· kg- 1 体重剂量的胸腺素α1 ( Tα1 ) ,对照组注射等量的生理盐水。试验期为 5 6d,观察 Tα1 对鸡体重、饲料转化率、法氏囊指数、胸腺指数、和脾脏指数的影响。结果表明 :在 3 5 ,42和 5 6日龄时 ,低剂量组鸡的体重显著高于其他各组 ( P<0 .0 5 ) ,而在 1 4和 2 1日龄时各组平均体重差异不显著 ( P>0 .0 5 )。在整个试验期间 ,低剂量组的饲料报酬均高于其他三组 ;低剂量组的法氏囊指数均高于其他三组 ,并在 1 4日龄时与对照组和中剂量组差异显著 ( P <0 .0 5 ) ,而在 3 5日龄与对照组和高剂量组差异显著 ( P <0 .0 5 ) ;低剂量组的胸腺指数也高于其他三组 ,1 4日龄时与对照组差异显著 ( P <0 .0 5 ) ,而与中剂量组和高剂量组差异不显著( P>0 .0 5 ) ,但在 3 5和 5 6日龄时差异显著 ( P <0 .0 5 ) ;低剂量组的脾脏指数高于其他三组 ,且在 3 5和 5 6日龄差异显著 ( P <0 .0 5 )。     

Formestane和17-βestradiol诱导鸡、鹌鹑胚胎性反转的研究

为了研究芳香化酶抑制剂(Formestane)和17β-雌二醇(17-βestradiol)对鸡和鹌鹑胚胎性分化的影响,试验根据家禽性别分化的机理,利用100μg/mL的Formestane和50μg/mL的17-βestradiol,通过胚蛋注射的方法对3日龄的鸡胚和2日龄的鹌鹑胚进行处理,观察Formestane和17-βestradiol对鹌鹑性别分化的影响。结果表明,注射Formestane和17-βestradiol的鸡胚和鹌鹑胚都发生了性反转。     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Lower Paleozoic oil relationships within Williston Basin, Canada

The Williston Basin is a significant petroleum province, containing oil production zones that include the Middle Cambrian to Lower Ordovician, Upper Ordovician, Middle Devonian, Upper Devonian and Mississippian and within the Jurassic and Cretaceous. The oils of the Williston Basin exhibit a wide range of geochemical characteristics defined as "oil families", although the geochemical signature of the Cambrian Deadwood Formation and Lower Ordovician Winnipeg reservoired oils does not match any "oil family". Despite their close stratigraphic proximity, it is evident that the oils of the Lower Palaeozoic within the Williston Basin are distinct. This suggests the presence of a new "oil family" within the Williston Basin. Diagnostic geochemical signatures occur in the gasoline range chromatograms, within saturate fraction gas chromatograms and biomarker fingerprints. However, some of the established criteria and cross-plots that are currently used to segregate oils into distinct genetic families within the basin do not always meet with success, particularly when applied to the Lower Palaeozoic oils of the Deadwood and Winnipeg Formation.     

An Analysis of Catherine＇s Character——Wuthering Heights

Wuthering Heights, Emily Bronte＇s only novel, was published in December of 1847 under the pseudonym Ellis Bell. The book did not gain immediate success, but it is now thought one of the finest novels in the English language. Catherine is the key character of this masterpiece, because everybody and everything center on her though she had a short life. We can understand this masterpiece better if we know Catherine well.     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

Design and analysis of generic LBS application developing platform

Location based services is promising due to its novel working style and contents.A software platform is proposed to provide application programs of typical location based services and support new applications developing efficiently. The analysis shows that this scheme is easy implemented, low cost and adapt to all kinds of mobile nework system.     

橡胶颗粒沥青混合料低温抗裂性试验研究

以AC-13级配为基础,将橡胶颗粒代替部分集料掺入混合料中,以低温弯曲试验为评价方法对不同橡胶颗粒掺量下沥青混合料的低温抗裂性进行研究,并引入应变能密度值对混合料的低温抗裂性进行综合评价.试验结果表明:橡胶颗粒沥青混合料试件的破坏微应变均超过2 300,满足冬寒区的技术指标;无论是否掺加橡胶颗粒,随着温度的下降,沥青混合料破坏时的最大弯拉强度增大,弯拉应变降低,劲度模量增大;弯曲应变能密度在胶粒掺量为1%左右时具有较大的弯曲应变能密度值,此时橡胶颗粒沥青混合料具有较好的低温抗裂性.     

A computer generator for randomly layered structures

AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

Quantitative trait loci （QTLs） for quality traits related to protein and starch in wheat

Quality traits in wheat （Triticum aestirum L.） were studied by quantitative trait locus （QTL） analysis in a recombinant inbred line （RIL） population, a set of 131 lines derived from Chuan 35050 × Shannong 483 cross （ChSh）. Grains from RILs were assayed for 21 quality traits related to protein and starch. A total of 35 putative QTLs for 19 traits with a single QTL explaining 7.99-40.52% of phenotypic variations were detected on 10 chromosomes, 1D, 2A, 2D, 3B, 3D, 5A, 6A, 6B, 6D, and 7B. The additive effects of 30 QTLs were positive, contributed by Chuan 35050, the remaining 5 QTLs were negative with the additive effect contributed by Shannong 483. For protein traits, 15 QTLs were obtained and most of them were located on chromosomes 1 D, 3B and 6D, while 20 QTLs for starch traits were detected and most of them were located on chromosomes 3D, 6B and 7B. Only 7 QTLs for protein and starch traits were co-located in three regions on chromosomes 1D, 2A and 2D. These protein and starch trait QTLs showed a distinct distribution pattern in certain regions and chromosomes. Twenty-two QTLs were clustered in 6 regions of 5 chromosomes. Two QTL clusters for protein traits were located on chromosomes 1D and 3B, respectively, three clusters for starch traits on chromosomes 3D, 6B and 7B, and one cluster including protein and starch traits on chromosome 1D.     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.