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1.
凤眼莲体内多酚氧化酶的生理化特性   总被引:1,自引:0,他引:1  
用无菌凤眼莲作材料,对其体内多酚氧化酶的生理化特性进行了较系统的研究,结果表明,凤眼莲体内的多酚氧化酶仅能氧化邻位二元酚;在酶的提取过程中,加入凤眼莲湿重1/10的PVP(聚乙烯吡咯烷酮)能保持多酚氧化酶的最大活性,酶氧化邻苯二酚的最适pH为6.8,对热不稳定,抗坏血酸和EDTA.2Na能明显地抑制酶的活性;多酚氧化酶主要存在于凤眼莲的线粒体内。  相似文献   

2.
以水稻为材料,研究了不同深度的模拟污水中N、P对幼苗过氧化氢酶(Catalase,CAT,EC1.11.1.6)、乙醇酸氧化酶(Glycolateoxidase,GO,EC1.3.3.1)、过氧化和物酶(Peroxidase,POD,EC1.11.1.7)和叶绿素含量的影响。结果表明:低浓度N、P浓度分别超过8.56mmol.L^-1和0-0.2mmol.L^-1范围)可诱导CAT活性增大,而后下  相似文献   

3.
马尾松毛虫和荔蝽体内多酚氧化酶的初步研究   总被引:8,自引:0,他引:8       下载免费PDF全文
为了寻找不污染环境的“生物合理途径”新型杀虫剂,研究了马尾松毛虫和荔蝽体内的多酚氧化酶。发现马尾松毛虫幼虫的多酚氧化酶活力与虫龄大小成正相关。多来宝(etofenprox)处理100min后,5龄幼虫的多酚氧化酶为正常虫体的3.6倍。荔蝽3龄若虫经乙醇处理10min后,多酚氧化酶活力为正常虫体的2.7倍;荔蝽3龄若虫经菟丝子(Cuscutajaponica)和夹竹桃(Neriumindicum)的混合醇提液处理60min,多酚氧化酶活力为正常虫体的2.4倍、经乙醇单独处理荔蝽3龄若虫后多酚氧化酶活力为正常虫体的2.7倍。  相似文献   

4.
保鲜剂对麝香石竹切花SOD和POD同工酶的影响   总被引:1,自引:0,他引:1       下载免费PDF全文
用聚丙烯酰胺凝胶电泳法分析麝香石竹(DianthuscaryophylusL.)切花的超氧物歧化酶(SOD)、过氧化物酶(POD)同工酶,结果表明保鲜剂可使SOD的活性增强,能保持SOD酶带不缺失和出现新的酶带;使POD活性比对照减弱。保鲜剂对SOD同工酶的影响比POD同工酶明显;对不同器官的酶活性和其同工酶有不同的效果  相似文献   

5.
采用聚丙烯酰胺凝胶电泳实验,对鸢尾蒜(Ixioliriontataricum)和新种准噶尔鸢尾蒜(I.songaricum)二种植物的根、鳞茎、地下茎、地上茎、基生叶及果实等器官的POD及Est同工酶进行了分析比较。结果表明:二种植物的POD及Est同工酶的酶带数目、Rf值和酶带相对活性均有很明显的差异;同一植物不同器官的同工酶具有一定的特异性;同时,本实验从同工酶方面为鸢尼蒜属新分类群的确定提供了新的依据。  相似文献   

6.
华北落叶松多酚氧化酶动力学特性及其同工酶的研究   总被引:8,自引:0,他引:8  
文中对华北落叶松根系多酚氧化酶动力学特性及其同工酶进行分析。结果表明:该酶对邻苯二酚(二元酚)和焦性没食子酸(三元酚)都能氧化,但对前者亲和力较强;pH7.0时酶活性最强;Cu2+提高多酚氧化酶活性;EDTA,H3BO3及半胱氨酸可抑制该酶活性;聚丙烯酰胺凝胶电泳将根系多酚氧化酶分出7条同工酶带。  相似文献   

7.
研究了分布于甘肃省临泽地区的沙拐枣、珍珠、霸王、泡泡刺、胡杨等5种沙生植物的光合酶:果糖-1.6-双磷酸酯酶(FDPase)、1.5-二磷酸核酮糖羧化酶(RUBPcase)、磷酸烯醇式丙酮酸羧化酶(PEPcase)、NAD-苹果酸脱氢酶(NAD-MDH)活性的季节变化。结果表明,珍珠和沙拐枣的FDPase酶活性远低于其它几种材料;珍珠、霸王、沙拐枣的PEPcase活性明显高于其它两种材料;珍珠、沙拐枣的NAD-MDH活性明显高于其它三种材料。可见,珍珠、霸王是CAM植物,沙拐枣是C4植物,胡杨是C3植物,泡泡刺有可能在干旱协迫下由C3向C4或CAM途径转化。  相似文献   

8.
植物净化塘处理油漆厂废水的试验研究   总被引:4,自引:0,他引:4  
凤眼莲(EichhorniaCrassipes)和细绿萍(Azollafiliculoides)对酸、醇、苯系物为主的工业污染水具有生物净化作用,以这两种植物为主体的净化塘工艺流程比较简便,具有工业应用潜力.凤眼莲的去污效果优于细绿萍.凤眼莲对酚(2d)、COD(6d)的去除率分别为97%、90%。对氨氮和油类的6d去除率为27%、49%,且稳定性比较差.本文还对凤眼莲净化污水的机理作了探讨.  相似文献   

9.
ACC氧化酶的测定方法   总被引:4,自引:0,他引:4  
利用番木瓜CaricaPapayaL.果实为材料,研究了1-氨基环丙烷-1-羧酸(ACC)氧化酶的体内和体外测定方法.在ACC氧化酶的体外分析中,甘油体积分数为10%~30%时能稳定酶的活性;φ(CO2)为0.3%~20%(反应体系气相中)和0.5~12mmol/LACC提高酶的活性;较适宜的pH值为7.2.  相似文献   

10.
白皮松形成层活动周期中过氧化物酶和酯酶同工酶的变化   总被引:10,自引:0,他引:10  
白皮松(PinusbungeanaZucc.)枝条不同组织中过氧化酶(POD)和酯酶(EST)同工酶的酶谱也不同,而且每种组织中这两种酶的同工酶酶谱随形成层活动周期的变化而变化。在休眠期,形成层区域过氧化物酶的同工酶酶带最多,这时周皮和韧皮部中酯酶同工酶谱在正极端出现一些酶带,这可能与抗寒有关。  相似文献   

11.
Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.  相似文献   

12.
何延凌 《科技信息》2008,(4):258-258
Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women's language from these perspectives: pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women's language, more attention should be paid to language use in social context. What's more, the linguistic phenomena in a speaking community can be understood more thoroughly.  相似文献   

13.
The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3~4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰~ -7.8‰, PDB) and matrix dolomites (-6.6‰~ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084~0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078~0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.  相似文献   

14.
理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。  相似文献   

15.
As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.  相似文献   

16.
AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…  相似文献   

17.
There are numerous geometric objects stored in the spatial databases. An importance function in a spatial database is that users can browse the geometric objects as a map efficiently. Thus the spatial database should display the geometric objects users concern about swiftly onto the display window. This process includes two operations:retrieve data from database and then draw them onto screen. Accordingly, to improve the efficiency, we should try to reduce time of both retrieving object and displaying them. The former can be achieved with the aid of spatial index such as R-tree, the latter require to simplify the objects. Simplification means that objects are shown with sufficient but not with unnecessary detail which depend on the scale of browse. So the major problem is how to retrieve data at different detail level efficiently. This paper introduces the implementation of a multi-scale index in the spatial database SISP (Spatial Information Shared Platform) which is generalized from R-tree. The difference between the generalization and the R-tree lies on two facets: One is that every node and geometric object in the generalization is assigned with a importance value which denote the importance of them, and every vertex in the objects are assigned with a importance value,too. The importance value can be use to decide which data should be retrieve from disk in a query. The other difference is that geometric objects in the generalization are divided into one or more sub-blocks, and vertexes are total ordered by their importance value. With the help of the generalized R-tree, one can easily retrieve data at different detail levels.Some experiments are performed on real-life data to evaluate the performance of solutions that separately use normal spatial index and multi-scale spatial index. The results show that the solution using multi-scale index in SISP is satisfying.  相似文献   

18.
In the 19th century the society was controlled by men, and women were just appendants of them, they had not any rights and freedom. But Jane was an exception, she showed some characteristics of early feminist. Jane showed her characteristics of feminism in three aspects: rebellion, equality, and independence. These characteristics were helpful to her success, and feminism is the only way out for women of that time.  相似文献   

19.
正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-  相似文献   

20.
We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-  相似文献   

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