山羊血浆环核苷酸在感染肝片吸虫前后的变化

用放射免疫方法测定山羊血浆ｃＡＭＰ，ｃＧＭＰ和ｃＡＭＰ／ｃＧＭＰ正常水平值以及感染肝片吸虫后的动态变化。结果表明：山羊血浆ｃＡＭＰ，ｃＧＭＰ和ｃＡＭＰ／ｃＧＭＰ的正常值分别为（０．８０±０．０３），（０．８３±０．０３） ｐｍ ｏｌ·ｍ Ｌ－ １，（０．６９±０．０４），感染１ 组和２ 组山羊在感染后第１～６ 周，血液中ｃＡＭＰ水平显著低于或低于对照组，以后两组水平相当。感染１ 组的ｃＧＭＰ水平在感染后５～９ 周期间低于对照组，而感染２ 组山羊的ｃＧＭＰ水平在感染后１～４ 周高于对照组。感染１ 组和２ 组山羊的ｃＡＭＰ／ｃＧＭＰ比值分别在感染后１～４ 周和１～６ 周呈现低于对照组的趋势，但差异不明显。因而，肝片吸虫感染可导致宿主的ｃＡＭＰ，ｃＧＭＰ和ｃＡＭＰ／ｃＧＭＰ紊乱。     

磺甲基化（丙烯酰胺／丙烯酸）共聚物的合成与性能研究

合成了磺甲基化（丙烯酰胺／丙烯酸）共聚物〔ｓｕｌｆｏｍｅｔｈｙｌａｔｅｄｐｏｌｙ（ａｃｒｙｌａｍｉｄｅ－ｃｏ－ａｃｒｙｌｉｃａｃｉｄ）,简称ＳＭＰ（ＡＭ／ＡＡ）〕,测定了不同磺化度ＳＭＰ（ＡＭ／ＡＡ）水溶液的粘度,研究了ＳＭＰ（ＡＭ／ＡＡ）的若干泥浆性能,用红外光谱对ＳＭＰ（ＡＭ／ＡＡ）及二元共聚物（丙烯酰胺／丙烯酸）〔ｐｏｌｙ（ａｃｒｙｌａｍｉｄｅ－ｃｏ－ａｃｒｙｌｉｃａｃｉｄ）,简称Ｐ（ＡＭ／ＡＡ）〕的结构作了表征．试验表明,磺甲基化（丙烯酰胺／丙烯酸）共聚物具有较好的降失水性能和抗钙侵性能,是一种较好的降失水剂．     

钙拮抗剂对肾性高血压肾脏细胞Ca^2＋超负荷的影响

将３０例大鼠随机分为正常对照组、手术组及手术后用药组手术组及手术后用药组通过钳夹大鼠双肾动脉制成肾血管性高血压动物模型，其中术后用药组给予钙拮抗剂，测定其肾小管细胞内Ｃａ２＋浓度，结果分别为：正常组ｘ＝（１．７２７±１．４２９）×１０－７ｍｏｌ／Ｌ（ｎ＝６），手术组ｘ＝（４２．８３１±２０．０８２）×１０－７ｍｏｌ／Ｌ（ｎ＝６），用药组ｘ＝（１８．５７５±７．０４５）×１０－７ｍｏｌ／Ｌ（ｎ＝６），统计分析Ｆ＝１４．０８，Ｐ＝０．０００４，在α＝０．０５水准，三组细胞内Ｃａ２＋浓度总的差别有统计学意义（Ｐ＜０．０１），两两比较：正常组与手术组Ｑ＝７．４６（Ｐ＜０．０１），正常组与用药组Ｑ＝３．５６（Ｐ〈０．０５），手术组与用药组Ｑ＝４．４（Ｐ＜０．０５），表明钙拮抗剂能部分阻止细胞内Ｃａ２＋浓度超负荷。     

重组人钙调素的表达及其McAb的制备与精子CaM定位

用基因重组技术构建人钙调素基因Ⅲ（ｈＣａＭⅢｃＤＮＡ）表达载体ｐＢＶ／ｈＣａＭⅢ,并在大肠杆菌ＤＨ５α中经热诱导获得可溶性ＣａＭ蛋白的高效表达．将纯化的重组ｈＣａＭ与异型双功能剂ＳＰＤＰ及鼠血清白蛋白（ＭＳＡ）交联,免疫Ｂａｌｂ／ｃ小鼠,用常规细胞融合技术制备单克隆抗体（ＭｃＡｂ）,得到３株稳定分泌单克隆抗体的杂交瘤细胞．间接ＥＬＩＳＡ和斑点免疫结果证实,三种单克隆抗体均与自制的ｒｈＣａＭ和ＣａＭ标准品起特异反应．用免疫组化法对精子中ＣａＭ定位,发现ＣａＭ主要分布于精子头颈部,不育组ＣａＭ＋精子率（（４５．０±７．５）％）显著低于生育组（（６８．５±１０．５）％）．     

黄粉虫对血清总胆固醇及甘油二酯的作用

利用黄粉虫的幼虫含较多亚油酸成分，喂正常迪卡公鸡，并对它的血清总胆固醇及甘油三酯的作用进行研究．试验动物随机分成３组，Ｉ组为对照组，Ⅱ组为黄粉虫幼虫组，６ｇ／ｄ，Ⅲ组为复方亚油酸乙酯丸组，１丸（含亚油酸乙酯３００ｍｇ）／ｄ，实验期１８ｄ．结果表明，血清总胆固醇，Ⅱ组为（２．７３±０．２６）ｍｍｏｌ／Ｌ，Ⅲ组为（２．８３±０．３１）ｍｍｏｌ／Ｌ，均明显低于对照组（３．４０±０．６）ｍｍｏｌ／Ｌ，Ｐ＜０．０１）．血清甘油三酯，Ⅱ组为（０．４０±０．１３）ｍｍｏｌ／Ｌ，Ⅲ组为（０．３６±０．１１）ｍｍｏｌ／Ｌ，均明显低于对照组（（０．９±０．３）ｍｍｏｌ／Ｌ，Ｐ＜０．０１）．试验说明用黄粉虫的幼虫喂迪卡公鸡有显著降低血清总胆固醇和甘油三酯作用．     

不同贮藏期鸡蛋中脂质过氧化物含量分析

用ＴＢＡ比色法测定了不同贮藏期鸡蛋蛋清及全蛋液中脂质过氧化物的含量。结果表明：随着贮藏时间的延长，鸡蛋全蛋液中脂质过氧化物的含量明显升高（Ｐ＜０．０１），但是蛋清中脂质过氧化物含量没有明显变化。新鲜鸡蛋全蛋液和蛋清中脂质过氧化物的含量分别为（２．７０±０．４８）、（０．３２４±０．０４７）μｍｏｌ（ＭＤＡ）／Ｌ全蛋液及蛋清中脂质过氧化物的含量在贮藏４５ｄ时分别为（４．１８±０．４７）、（０．３３６±０．０４４）μｍｏｌ（ＭＤＡ）／Ｌ；在贮藏７０ｄ时分别为（５．３２±０．６３）、（０．３６４±０．０４７）μｍｏｌ（ＭＤＡ）／Ｌ。     

α_1-肾上腺素受体激活防止心室颤动机理的研究

在氯仿诱发小鼠心室颤动（ＶＦ）的模型上，研究了激动α１－肾上腺素能受体（ＡＲ）防止ＶＦ发生的机制．甲氧胺（１５ｍｇ／ｋｇ，ｉｐ）可防止ＶＦ，哌唑嗪（５ｍｇ／ｋｇ）和硝苯啶（３ｍｇ／ｋｇ）阻断甲氧胺的防颤作用，阿托品（１５ｍｇ／ｋｇ）和育亨宾（５ｍｇ／ｋｇ）无效．放免测定ＶＦ组（ｎ＝８）与甲氧胺组（ｎ＝８）心室肌环核苷酸含量，与ＶＦ组比．ｃＡＭＰ水平无变化，ｃＧＭＰ含量显著增加（ｐ＜０．００１），ｃＡＭＰ／ｃＧＭＰ比值显著减小（ｐ＜０．００１）．提示，甲氧胺防止ＶＦ是通过激动心肌α１－ＡＲ介导的，并与心肌ｃＧＭＰ水平提高有关．     

ABS/PA6/St-co-NPMI共混体系相界面特征研究

用小角Ｘ光散射法（ＳＡＸＳ）对ＡＢＳ树脂（丙烯腈Ａ－丁二烯Ｂ－苯乙烯Ｓ）／聚酰胺６树脂（ＰＡ６）／苯乙烯－马来酰胺共聚物（Ｓｔ－ｃｏ－ＮＰＭＩ）三元共混体系的界面结构进行了研究。由Ｄｅｂｙｅ散射理论中相关距离（ａｃ）和积分不变量（Ｑ）值随组成比变化显示,在ＡＢＳ／ＰＡ６／Ｓｔ－ｃｏ－ＮＰＭＩ（７０／３０／２,ｗｔ％）时,ａｃ＝５０．７４,Ｑ＝０．３９７２体系相容程度较高。这表明,Ｓｔ－ｃｏ－ＮＰ     

化疗并用云芝糖肽对卵巢和子宫体癌患者免疫功？…

对４０例手术确诊的卵巢和子宫体癌患者，按期别及年龄配对分成２组，各２０例。Ａ组（试验组）接受化疗加云芝糖肽。Ｂ组（对照组）单纯化疗，观察两组治疗前后血免疫指标及生活质量指数的变化。结果治疗前后Ｋａｒｎｏｆｓｋｙ评分值Ａ组分别为８４±９．６分及９０±８．６分（Ｐ〈０．０５），Ｂ组分别为８３±９．２分及８０±１２．８分（Ｐ〉０．０５），治疗后Ａ比Ｂ组高１０分（Ｐ〈０．０５）；治疗后Ａ组和Ｂ组的ＣＤ＾＋     

犬冠状动脉周期性血流减少时冠状窦内血浆TXB_2及6-K-PGF_(1α)升高的意义

目的：观察冠状动脉（冠脉）发生周期性血流减少（ＣＦＲｓ）时血栓烷Ａ２（ＴＸＡ２）及前列环素（ＰＧＩ２）的变化及意义。方法：犬剖胸分离出２～３ｃｍ长的冠脉前降支（ＬＡＤ）,用血管钳钳夹方法损伤冠脉内膜后放置血管缩窄环,使冠脉达临界狭窄。缩窄环近端置电磁流量计,诱发ＣＦＲｓ,在ＣＦＲｓ出现前及ＣＦＲｓ出现后４０ｍｉｎ冠状窦内采血,用放免法测ＴＸＡ２及ＰＧＩ２的代谢产物ＴＸＢ２及６－Ｋ－ＰＧＦ１α。结果：ＣＦＲｓ出现４０ｍｉｎ后,ＴＸＢ２及６－Ｋ－ＰＧＦ１α均显著升高（Ｐ＜０．０１）（ＴＸＢ２从７５．６２±４８．８７ｎｇ／Ｌ升至１０９．２２±４５．９９ｎｇ／Ｌ;６－Ｋ－ＰＧＦ１α从１６．１４±９．２８ｎｇ／Ｌ升至３５．５７±１７．２１ｎｇ／Ｌ）。结论：发生ＣＦＲｓ时,血管内皮细胞分泌的６－Ｋ－ＰＧＦ１α随血小板分泌的ＴＸＢ２一同显著升高,其机理可能是血管内皮细胞的一种应激性反应。     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Lower Paleozoic oil relationships within Williston Basin, Canada

The Williston Basin is a significant petroleum province, containing oil production zones that include the Middle Cambrian to Lower Ordovician, Upper Ordovician, Middle Devonian, Upper Devonian and Mississippian and within the Jurassic and Cretaceous. The oils of the Williston Basin exhibit a wide range of geochemical characteristics defined as "oil families", although the geochemical signature of the Cambrian Deadwood Formation and Lower Ordovician Winnipeg reservoired oils does not match any "oil family". Despite their close stratigraphic proximity, it is evident that the oils of the Lower Palaeozoic within the Williston Basin are distinct. This suggests the presence of a new "oil family" within the Williston Basin. Diagnostic geochemical signatures occur in the gasoline range chromatograms, within saturate fraction gas chromatograms and biomarker fingerprints. However, some of the established criteria and cross-plots that are currently used to segregate oils into distinct genetic families within the basin do not always meet with success, particularly when applied to the Lower Palaeozoic oils of the Deadwood and Winnipeg Formation.     

An Analysis of Catherine＇s Character——Wuthering Heights

Wuthering Heights, Emily Bronte＇s only novel, was published in December of 1847 under the pseudonym Ellis Bell. The book did not gain immediate success, but it is now thought one of the finest novels in the English language. Catherine is the key character of this masterpiece, because everybody and everything center on her though she had a short life. We can understand this masterpiece better if we know Catherine well.     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

Design and analysis of generic LBS application developing platform

Location based services is promising due to its novel working style and contents.A software platform is proposed to provide application programs of typical location based services and support new applications developing efficiently. The analysis shows that this scheme is easy implemented, low cost and adapt to all kinds of mobile nework system.     

橡胶颗粒沥青混合料低温抗裂性试验研究

以AC-13级配为基础,将橡胶颗粒代替部分集料掺入混合料中,以低温弯曲试验为评价方法对不同橡胶颗粒掺量下沥青混合料的低温抗裂性进行研究,并引入应变能密度值对混合料的低温抗裂性进行综合评价.试验结果表明:橡胶颗粒沥青混合料试件的破坏微应变均超过2 300,满足冬寒区的技术指标;无论是否掺加橡胶颗粒,随着温度的下降,沥青混合料破坏时的最大弯拉强度增大,弯拉应变降低,劲度模量增大;弯曲应变能密度在胶粒掺量为1%左右时具有较大的弯曲应变能密度值,此时橡胶颗粒沥青混合料具有较好的低温抗裂性.     

A computer generator for randomly layered structures

AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

Quantitative trait loci （QTLs） for quality traits related to protein and starch in wheat

Quality traits in wheat （Triticum aestirum L.） were studied by quantitative trait locus （QTL） analysis in a recombinant inbred line （RIL） population, a set of 131 lines derived from Chuan 35050 × Shannong 483 cross （ChSh）. Grains from RILs were assayed for 21 quality traits related to protein and starch. A total of 35 putative QTLs for 19 traits with a single QTL explaining 7.99-40.52% of phenotypic variations were detected on 10 chromosomes, 1D, 2A, 2D, 3B, 3D, 5A, 6A, 6B, 6D, and 7B. The additive effects of 30 QTLs were positive, contributed by Chuan 35050, the remaining 5 QTLs were negative with the additive effect contributed by Shannong 483. For protein traits, 15 QTLs were obtained and most of them were located on chromosomes 1 D, 3B and 6D, while 20 QTLs for starch traits were detected and most of them were located on chromosomes 3D, 6B and 7B. Only 7 QTLs for protein and starch traits were co-located in three regions on chromosomes 1D, 2A and 2D. These protein and starch trait QTLs showed a distinct distribution pattern in certain regions and chromosomes. Twenty-two QTLs were clustered in 6 regions of 5 chromosomes. Two QTL clusters for protein traits were located on chromosomes 1D and 3B, respectively, three clusters for starch traits on chromosomes 3D, 6B and 7B, and one cluster including protein and starch traits on chromosome 1D.