紫外光固化在微流控芯片技术中的应用研究进展

报道了紫外光(UV)固化在微流控芯片技术中的应用,利用光刻技术可直接在聚合物材料上制作微通道,以减少微流控芯片的研究成本和制作周期。重点介绍了UV固化在微流控芯片发展过程中的光刻、曝光及表面改性等方面的应用及其特点,综述了近些年来UV固化在微流控芯片技术中的国内外研究现状,为微流控芯片的制备、发展及完善提供了一条可行的路径。最后在总结国内外研究现状的基础上展望了UV固化未来的发展趋势。     

微流控芯片在生物化学分新中的应用

在介绍微流控芯片基本特征的基础上,阐述了微流控芯片的独特优势,并从5个方面探讨了微流控芯片在生物化学分析中的应用.     

多器官微流控芯片技术及其应用

器官微流控芯片技术通过模拟人体器官内环境来实现接近体内的体外细胞培养.基于微流控芯片器官模型的发展,构建多器官芯片整合的微流控系统为研究器官之间物质(如药物和外源性污染物)的代谢作用提供了崭新的平台.本文从不同的多细胞共培养系统角度介绍了构建多器官微流控芯片模型以及在预测和评估药物代谢及其对人体毒性测试等领域的应用,并对多器官微流控芯片技术的发展进行总结和展望.     

微流控技术及其应用与发展

微流控技术广泛应用于生化分析、疾病诊断、微创外科手术、环境检测等领域。微通道结构的设计与制造、微纳尺度流体的驱动与控制、微流控器件及系统的集成与封装是该领域的3大关键技术。本文综述了微流控技术在这3个方面的发展现状及在不同领域中的应用,展望了微流控技术的发展前景,指出多相微流体的介观传输理论及跨尺度流体的性质将是今后研究的重点与热点。     

在PDMS-玻璃微流控芯片上的细胞培养

微流控芯片上的细胞培养技术是构建一个整体化的片上细胞分析实验室的基础。针对芯片上细胞培养存活率低、容易污染等问题,该文提出了一套polydimethylsiloxane(PDMS)-玻璃微流控芯片的制备、处理方法及片上细胞培养的操作流程,并讨论了影响微管道细胞培养的因素,包括准备细胞悬液、避免气泡、芯片表面处理和培养液中的血清浓度等。应用这套方法,在微流控芯片上成功培养了多种哺乳动物细胞。     

基于微流控技术新型光开关器件设计

首先介绍了较为常用的几种光开关的工作原理、特点和应用.描述了MEMS、MOEMS、微流控技术.以MOEMS及微流控技术相接合,提出一种新的微流控光开关阵列结构,通过电控方法改变微流控芯片波导结构,实现光路变化.该光开关具有体积小,结构简单,功率损耗低,有利于光开关阵列集成化、微型化及制作.以电控方式控制光开关,在开关速度上比温控更快、更稳定,且控制电路简单等特点易构成大容量交换矩阵.     

微流控芯片在生物化学分析中的应用

在介绍微流控芯片基本特征的基础上，阐述了微流控芯片的独特优势，并从5个方面探讨了微流控芯片在生物化学分析中的应用。     

滤纸微流控设备集成电化学检测

近年来,人们发现滤纸微流控设备相比于传统的微流控设备来说具有一次性使用、制作简单且成本更低的优点。简单综述了微流控设备的发展现状以及滤纸和电化学检测的相关特点,着重阐明了滤纸在微流控电化学检测中的应用,同时与商品化的检测仪相结合,为微流控设备未来商品化打下了坚实的基础。     

基于微流控的细胞操纵方法与应用

 微流控技术可实现微量液体的精密操控和多种功能模块的微型化集成,在细胞操纵方面具有精确、高效和低成本等传统手段无法比拟的优点,近些年成为学科交叉领域的研究热点之一。从细胞操纵的原理方法及应用出发,综述了微流控细胞操纵领域的最新研究进展,探讨、总结了各种细胞操纵方法的优缺点,并展望了微流控细胞操纵技术的发展趋势。     

集成琼脂糖结构的微流芯片在菌群研究中的应用

介绍集成琼脂糖结构的微流芯片的制作过程及其在菌群培养中的应用。设计了两种集成琼脂糖结构的微流芯片。第一种结构的芯片是恒化器（chemostat）微流芯片,可以用于研究杀菌动力学。这种微流芯片具有实现实时检测,简化实验操作,可以输出耐药菌等优点,有潜力成为研究杀菌动力学的一种方法。为了获得与细菌数目有关的更多信息,第二种结构的芯片集成3个不同高度的培养室,采用测量荧光强度和光密度值（optical density value, OD value）的方法来表征不同高度培养室的细菌数目。结果表明,这两种方法测量得到的细菌生长趋势基本上是一致的。这两种结构的芯片具有易于加工,限制细菌在培养室内生长而且允许营养物质自由扩散等优点,为研究可游动细菌提供了一个简便的方法。     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Lower Paleozoic oil relationships within Williston Basin, Canada

The Williston Basin is a significant petroleum province, containing oil production zones that include the Middle Cambrian to Lower Ordovician, Upper Ordovician, Middle Devonian, Upper Devonian and Mississippian and within the Jurassic and Cretaceous. The oils of the Williston Basin exhibit a wide range of geochemical characteristics defined as "oil families", although the geochemical signature of the Cambrian Deadwood Formation and Lower Ordovician Winnipeg reservoired oils does not match any "oil family". Despite their close stratigraphic proximity, it is evident that the oils of the Lower Palaeozoic within the Williston Basin are distinct. This suggests the presence of a new "oil family" within the Williston Basin. Diagnostic geochemical signatures occur in the gasoline range chromatograms, within saturate fraction gas chromatograms and biomarker fingerprints. However, some of the established criteria and cross-plots that are currently used to segregate oils into distinct genetic families within the basin do not always meet with success, particularly when applied to the Lower Palaeozoic oils of the Deadwood and Winnipeg Formation.     

An Analysis of Catherine＇s Character——Wuthering Heights

Wuthering Heights, Emily Bronte＇s only novel, was published in December of 1847 under the pseudonym Ellis Bell. The book did not gain immediate success, but it is now thought one of the finest novels in the English language. Catherine is the key character of this masterpiece, because everybody and everything center on her though she had a short life. We can understand this masterpiece better if we know Catherine well.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

Design and analysis of generic LBS application developing platform

Location based services is promising due to its novel working style and contents.A software platform is proposed to provide application programs of typical location based services and support new applications developing efficiently. The analysis shows that this scheme is easy implemented, low cost and adapt to all kinds of mobile nework system.     

橡胶颗粒沥青混合料低温抗裂性试验研究

以AC-13级配为基础,将橡胶颗粒代替部分集料掺入混合料中,以低温弯曲试验为评价方法对不同橡胶颗粒掺量下沥青混合料的低温抗裂性进行研究,并引入应变能密度值对混合料的低温抗裂性进行综合评价.试验结果表明:橡胶颗粒沥青混合料试件的破坏微应变均超过2 300,满足冬寒区的技术指标;无论是否掺加橡胶颗粒,随着温度的下降,沥青混合料破坏时的最大弯拉强度增大,弯拉应变降低,劲度模量增大;弯曲应变能密度在胶粒掺量为1%左右时具有较大的弯曲应变能密度值,此时橡胶颗粒沥青混合料具有较好的低温抗裂性.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

Quantitative trait loci （QTLs） for quality traits related to protein and starch in wheat

Quality traits in wheat （Triticum aestirum L.） were studied by quantitative trait locus （QTL） analysis in a recombinant inbred line （RIL） population, a set of 131 lines derived from Chuan 35050 × Shannong 483 cross （ChSh）. Grains from RILs were assayed for 21 quality traits related to protein and starch. A total of 35 putative QTLs for 19 traits with a single QTL explaining 7.99-40.52% of phenotypic variations were detected on 10 chromosomes, 1D, 2A, 2D, 3B, 3D, 5A, 6A, 6B, 6D, and 7B. The additive effects of 30 QTLs were positive, contributed by Chuan 35050, the remaining 5 QTLs were negative with the additive effect contributed by Shannong 483. For protein traits, 15 QTLs were obtained and most of them were located on chromosomes 1 D, 3B and 6D, while 20 QTLs for starch traits were detected and most of them were located on chromosomes 3D, 6B and 7B. Only 7 QTLs for protein and starch traits were co-located in three regions on chromosomes 1D, 2A and 2D. These protein and starch trait QTLs showed a distinct distribution pattern in certain regions and chromosomes. Twenty-two QTLs were clustered in 6 regions of 5 chromosomes. Two QTL clusters for protein traits were located on chromosomes 1D and 3B, respectively, three clusters for starch traits on chromosomes 3D, 6B and 7B, and one cluster including protein and starch traits on chromosome 1D.     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.