共查询到20条相似文献,搜索用时 15 毫秒
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A lentivirus-based system to functionally silence genes in primary mammalian cells,stem cells and transgenic mice by RNA interference 总被引:87,自引:0,他引:87
Rubinson DA Dillon CP Kwiatkowski AV Sievers C Yang L Kopinja J Rooney DL Zhang M Ihrig MM McManus MT Gertler FB Scott ML Van Parijs L 《Nature genetics》2003,33(3):401-406
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RNA polymerase is poised for activation across the genome 总被引:2,自引:0,他引:2
Muse GW Gilchrist DA Nechaev S Shah R Parker JS Grissom SF Zeitlinger J Adelman K 《Nature genetics》2007,39(12):1507-1511
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Systematic determination of genetic network architecture. 总被引:39,自引:0,他引:39
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Adaptive evolution of a duplicated pancreatic ribonuclease gene in a leaf-eating monkey 总被引:26,自引:0,他引:26
Although the complete genome sequences of over 50 representative species have revealed the many duplicated genes in all three domains of life, the roles of gene duplication in organismal adaptation and biodiversity are poorly understood. In addition, the evolutionary forces behind the functional divergence of duplicated genes are often unknown, leading to disagreement on the relative importance of positive Darwinian selection versus relaxation of functional constraints in this process. The methodology of earlier studies relied largely on DNA sequence analysis but lacked functional assays of duplicated genes, frequently generating contentious results. Here we use both computational and experimental approaches to address these questions in a study of the pancreatic ribonuclease gene (RNASE1) and its duplicate gene (RNASE1B) in a leaf-eating colobine monkey, douc langur. We show that RNASE1B has evolved rapidly under positive selection for enhanced ribonucleolytic activity in an altered microenvironment, a response to increased demands for the enzyme for digesting bacterial RNA. At the same time, the ability to degrade double-stranded RNA, a non-digestive activity characteristic of primate RNASE1, has been lost in RNASE1B, indicating functional specialization and relaxation of purifying selection. Our findings demonstrate the contribution of gene duplication to organismal adaptation and show the power of combining sequence analysis and functional assays in delineating the molecular basis of adaptive evolution. 相似文献
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CpG island hypermethylation is maintained in human colorectal cancer cells after RNAi-mediated depletion of DNMT1 总被引:6,自引:0,他引:6
The role of the primary mammalian DNA methyltransferase, DNMT1, in maintaining CpG island methylation in human colon cancer cells has recently been questioned. This controversy has arisen from discrepancies between genetic knockout and RNA interference-mediated knockdown studies. Here, we re-examined the RNA interference-based approach and found that hypermethylation of single-copy genes is maintained in cells transiently and stably depleted of DNMT1. 相似文献
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A gene expression map of Arabidopsis thaliana development 总被引:3,自引:0,他引:3
Schmid M Davison TS Henz SR Pape UJ Demar M Vingron M Schölkopf B Weigel D Lohmann JU 《Nature genetics》2005,37(5):501-506