不同盐度对三角帆蚌钩介幼虫、稚贝生理活动和存活的影响

对三角帆蚌钩介幼虫、不同日龄稚贝在不同盐度条件下的生理活动和存活率进行了观察和比较。以淡水组作为对照组,试验结果显示:三角帆蚌钩介幼虫在淡水组48 h存活率达90%,而盐度2条件下,2 h内即全部死亡;同龄的稚贝存活率随生理盐水浓度、实验时间的增加而逐渐降低,生理活动明显受到盐度的影响,表现为心跳频率降低,肠道蠕动减弱,斧足运动减少;随作用时间的增加,稚贝表现为心跳停止、双壳张开死亡;大日龄稚贝在同盐度下的存活率显著高于低日龄稚贝,7日龄稚贝在48 h内所有盐度组全部死亡,45日龄稚贝仅在盐度为2试验液中有90%存活,但生理活动明显低于正常淡水组,表现为心跳微弱,无肠道斧足运动。因此,认为三角帆蚌钩介幼虫对盐度较敏感,其耐受力低于稚贝,而三角帆蚌稚贝的适宜盐度要低于2。     

西施舌人工育苗及稚贝培育技术的研究

选择野生 3龄西施舌 ,采取人工育肥、刺激产卵、选优培育、盘架式立体采苗、盘架式立体培育稚贝等技术工艺 ,培育出体长 3 .1～ 7.2mm幼贝 2 0 0 .9× 1 0 4粒 ,平均 3 .5 45× 1 0 4粒 /m2 .2 0 0 0年 9月2 6日 ,在陆上高位池 ,蓄养平均体长 9.8mm ,平均体重 0 .2 g的幼贝 2 0 .6× 1 0 4粒 ,以单细胞藻类为饵料 ,2 0 0 1年 7月 1日培育成功 ,平均体长 3 9.0 5mm ,平均体重 9.87g的 1龄西施舌幼贝 3 .1 0 5× 1 0 4粒 ,成活率为1 5 .0 7% .     

西施舌稚贝对3种微藻选择性及摄食率研究

对西施舌稚贝的摄食率及藻类选择性变化进行研究.结果表明,西施舌稚贝对直径11.2 μm四爿藻具有较高的选择性,而对云霄微型藻和牟氏角毛藻的摄食率均偏低.西施舌稚贝对四爿藻的摄食率在第5 h时达到最高值(174.928×10-5mg·h-1).当西施舌稚贝分别摄食3种藻类时,在适宜的藻类密度范围内,摄食率均随着藻类密度的增大而迅速增加,当藻类密度达到一定值后,摄食率呈下降趋势.西施舌稚贝摄食率与藻类密度之间关系适用二次多项式表示.     

pGH基因全序列ApaⅠ酶切位点多态性与生产性能的相关分析

本实验利用PCR-RFLP技术分析了约克夏和长白猪pGH基因ApaⅠ酶切位点多态性及其与生产性能的关系.结果表明,在pGH基因序列中共检测到5种基因型,4种等位基因,基因型频率和等位基因频率在2个猪品种间分布差异不显著;约克夏ApaⅠ酶切多态位点中,AC基因型个体在165日龄体重和70-165日龄日增重上显著高于AA基因型个体(P<0.1);但长白猪ApaⅠ多态位点不同基因型在这两个生产性状上的差异不显著.     

大獭蛤苗种池塘中间培育试验

2002年9月至2003年4月,在广西海洋研究所古城基地进行大獭蛤（LutmriamaximaJonas）苗种池塘中间培育试验。试验在铺沙的池塘里进行,放养的大獭蛤苗种规格为壳长0．2～0．8mm,大獭蛤稚贝饵料为施肥繁殖的浮游藻类,以大部分大獭蛤稚贝壳长达到2cm为出苗标准。结果表明,在水温为20～30℃、盐度为zo％。以上,放苗量为375万粒／公顷的条件下,大獭蛤稚贝经过45～60d即可以培育成壳长为2cm左右的大规格苗种。     

关于放逸短沟蜷的生态研究

通过野外调查和室内实验，阐述了日本产放逸短沟蜷在不同生态环境一体内稚贝保有状况以及稚贝产出时期与水温的关系，结果表明，雌性个体的育儿囊中稚贝含有量夏季和冬季多，春季和秋季急剧减少。     

温度在饲育条件下对Semisulcopira decipiens的产仔数和稚贝生长的影响

在饲育条件下研究了S．decipiens的产仔数和稚贝生长及与水温的关系。结果表明:水温在上升期和下降期,新生贝和稚贝的生长速度较快,产仔数也较多,成体贝在水温17℃-31℃时,都可以产生新生贝,以21℃-25℃时产出较多,在高温条件下,成体贝的生长速度缓慢。     

水稻三个酯酶同工酶基因的遗传

用聚丙烯酰胺凝胶电泳和淀粉凝胶电泳对139个水稻品种进行酯酶同工酶的遗传分析表明,阳极具有酶带14条,阴极3条.阴极酶带2C和4C受Est_1两个共显性等位基因控制.杂种是阴极酶带3条表明阴极的酯酶可能是二(?)体.阳极酶带7A受Est_2基因上一个显性等位基因控制,12A,13A则受Est_3基因上两个共显性等位基因控制.Est_2和Est_3上的无效等位基因可能控制无效变异体(null variant).Est_1和Est_3、Est_2和Est_3之间未发现连锁关系.     

碱性脂肪酶产生菌--扩展青霉K40原生质体的制备和再生

采用 0 .6%纤维素酶加 0 .6%蜗牛酶的混合酶由扩展青霉 K4 0中获得大量的原生质体 ,并比较了菌龄、二硫苏糖醇 ( DTT)预处理方式、酶解时间、温度、 p H、培养基成分及稳定剂等因素对原生质体的形成和再生的作用 .选出制备原生质体的最适条件 :0 .6%纤维素酶 +0 .6%蜗牛酶 ,在 0 .6mol/L Na Cl+0 .3 %Ca Cl2 ,DTT预处理 0 .5h,菌龄为 1 9～ 2 0 h,p H 5.4 ,2 8℃酶解 3～ 3 .5h,原生质体产量可达 2 .3 6× 1 0 7个/ml,实现再生 ,再生率达 70 %～ 80 % .并对原生质体的释放和再生方式进行跟踪观察     

单胞藻投饵量对西施舌稚贝摄食率的影响

实验室内用静水实验方法研究西施舌稚贝的摄食率与单胞藻投饵量、摄食时间的关系,结果表明单胞藻投饵量在适宜的范围之内,随着投饵量的增加,摄食率也随之增加,而超过一定范围时,摄食率有所下降,西施舌稚贝的摄食率在投喂饵料2 h后逐步下降.     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Lower Paleozoic oil relationships within Williston Basin, Canada

The Williston Basin is a significant petroleum province, containing oil production zones that include the Middle Cambrian to Lower Ordovician, Upper Ordovician, Middle Devonian, Upper Devonian and Mississippian and within the Jurassic and Cretaceous. The oils of the Williston Basin exhibit a wide range of geochemical characteristics defined as "oil families", although the geochemical signature of the Cambrian Deadwood Formation and Lower Ordovician Winnipeg reservoired oils does not match any "oil family". Despite their close stratigraphic proximity, it is evident that the oils of the Lower Palaeozoic within the Williston Basin are distinct. This suggests the presence of a new "oil family" within the Williston Basin. Diagnostic geochemical signatures occur in the gasoline range chromatograms, within saturate fraction gas chromatograms and biomarker fingerprints. However, some of the established criteria and cross-plots that are currently used to segregate oils into distinct genetic families within the basin do not always meet with success, particularly when applied to the Lower Palaeozoic oils of the Deadwood and Winnipeg Formation.     

An Analysis of Catherine＇s Character——Wuthering Heights

Wuthering Heights, Emily Bronte＇s only novel, was published in December of 1847 under the pseudonym Ellis Bell. The book did not gain immediate success, but it is now thought one of the finest novels in the English language. Catherine is the key character of this masterpiece, because everybody and everything center on her though she had a short life. We can understand this masterpiece better if we know Catherine well.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

Design and analysis of generic LBS application developing platform

Location based services is promising due to its novel working style and contents.A software platform is proposed to provide application programs of typical location based services and support new applications developing efficiently. The analysis shows that this scheme is easy implemented, low cost and adapt to all kinds of mobile nework system.     

橡胶颗粒沥青混合料低温抗裂性试验研究

以AC-13级配为基础,将橡胶颗粒代替部分集料掺入混合料中,以低温弯曲试验为评价方法对不同橡胶颗粒掺量下沥青混合料的低温抗裂性进行研究,并引入应变能密度值对混合料的低温抗裂性进行综合评价.试验结果表明:橡胶颗粒沥青混合料试件的破坏微应变均超过2 300,满足冬寒区的技术指标;无论是否掺加橡胶颗粒,随着温度的下降,沥青混合料破坏时的最大弯拉强度增大,弯拉应变降低,劲度模量增大;弯曲应变能密度在胶粒掺量为1%左右时具有较大的弯曲应变能密度值,此时橡胶颗粒沥青混合料具有较好的低温抗裂性.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

Quantitative trait loci （QTLs） for quality traits related to protein and starch in wheat

Quality traits in wheat （Triticum aestirum L.） were studied by quantitative trait locus （QTL） analysis in a recombinant inbred line （RIL） population, a set of 131 lines derived from Chuan 35050 × Shannong 483 cross （ChSh）. Grains from RILs were assayed for 21 quality traits related to protein and starch. A total of 35 putative QTLs for 19 traits with a single QTL explaining 7.99-40.52% of phenotypic variations were detected on 10 chromosomes, 1D, 2A, 2D, 3B, 3D, 5A, 6A, 6B, 6D, and 7B. The additive effects of 30 QTLs were positive, contributed by Chuan 35050, the remaining 5 QTLs were negative with the additive effect contributed by Shannong 483. For protein traits, 15 QTLs were obtained and most of them were located on chromosomes 1 D, 3B and 6D, while 20 QTLs for starch traits were detected and most of them were located on chromosomes 3D, 6B and 7B. Only 7 QTLs for protein and starch traits were co-located in three regions on chromosomes 1D, 2A and 2D. These protein and starch trait QTLs showed a distinct distribution pattern in certain regions and chromosomes. Twenty-two QTLs were clustered in 6 regions of 5 chromosomes. Two QTL clusters for protein traits were located on chromosomes 1D and 3B, respectively, three clusters for starch traits on chromosomes 3D, 6B and 7B, and one cluster including protein and starch traits on chromosome 1D.     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.