| 何小珍,贺冬秀,喻翠云,高治平,郭玉.野菊叶中绿原酸及木犀草素-7-O-葡萄糖苷测定[J].南华大学学报(自然科学版),2010,24(4):95~98.[HE Xiao-zhen,HE Dong-xiu,YU Cui-yun,GAO Zhi-ping,GUO Yu.Determination of Chlorogcnic Acid and Luteolin-7-O-glycoside in the Leaf of Flos Chrysanthemi indici[J].Journal of University of South China(Science and Technology),2010,24(4):95~98.] |
| 野菊叶中绿原酸及木犀草素-7-O-葡萄糖苷测定 |
| Determination of Chlorogcnic Acid and Luteolin-7-O-glycoside in the Leaf of Flos Chrysanthemi indici |
| 投稿时间：2010-07-17 |
| DOI： |
| 中文关键词: 野菊叶 绿原酸 木犀草素-7-O-葡萄糖苷；高效液相色谱 |
| 英文关键词:leaves of Flos Chrysanthemi indici chlorogcnic acid luteolin-7-O- glycosides HPLC |
| 基金项目:湖南省教育厅基金资助项目(10C1179) |
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| 中文摘要: |
| 建立应用高效液相色谱测定野菊叶中绿原酸及木犀草素-7-O-葡萄糖苷含量的方法.以0.04%磷酸-乙腈为流动相,流速为1.0 mL/min,梯度洗脱,检测波长355 nm,结果表明绿原酸浓度10-100 μg/mL范围内线性关系良好,相关系数r=0.997;平均回收率为96.7%,相对标准偏差（RSD）为0.85%（n=6）.木犀草素-7-O-葡萄糖苷浓度1-10 μg/mL范围内线性关系良好,r=0.999;平均回收率为92.6%,RSD为0.63%（n=6）. |
| 英文摘要: |
| The contents of chlorogcnic acid and luteolin-7-O- glycosides in the leaf of Flos Chrysanthemi indici were determined by HPLC at 355 nm with the flow rate 1.0 mL/min,while acetonitril-0.04% H3PO4 was used as the mobile phase with gradient elution.The concentration of chlorogcnic acid within the range of 10-100 μg/mL had a good linearity with r=0.997.The average recovery of chlorogcnic acid was 96.7% and its relative standard deviation (RSD) was 0.85% (n=6).The concentration of Luteolin-7-O- glycosides within the range of 1-10μg/mL had a good linearity with r=0.999.The average recovery of Chlorogcnic acid was 92.6% and its relative standard deviation (RSD) was 0.63% (n=6).The method is simple,sensitive,accurate and reproducible,suitable for the simultaneous determination of chlorogcnic acid and luteolin-7-O-glycosides in leaves of Flos Chrysanthemi indici. |
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