厌气混合培养中产甲烷菌和硫酸盐还原菌的动力学竞争Ⅱ.动力学推定的结果及讨论

根据前文建立的动力学模型及批量实验结果,进行了包括乙酸及氢气利用在内的MPB和SRB的动力学常数的推定.结果显示,以Methanothrix及Methanobacter为种属的乙酸和氢气利用MPB;以Desulfobacter postageix和Desulfovibrior为种属的乙酸和氢气利用SRB分别存在于葡萄糖消化过程中.在反映现实情况的低浓度硫酸根离子条件下,热力学及动力学均占优势的乙酸利用SRB相反完全竞争不过乙酸利用MPB.这是由于对硫酸根离子乙酸利用SRB竞争不过氢气利用SRB的缘故,因为后者(Ks=10.7～13.3mg/L)具有比前者(K=19.5～21.4mg/L)更高的硫酸根离子亲合性.这一结果进一步显示有必要重新评价长久以来一直承担负面角色的SRB的作用.因为如果从有利于厌气过程中甲烷主要产生者-乙酸利用MPB的生长这一角度来看,适量生长的氢气利用SRB能够完全抑止作为对手的乙酸利用SRB的生长以至发挥重大的正面作用.另外,该研究提供的动力学推定方法能简便、快速、准确的推定出微生物菌体浓度和诸动力学常数,对于生物处理的近乎所有过程都具有参考价值.

Kinetic Competition Between Methanogenic and Sulfate-Reducing Bacteria in Anaerobic Mixed Cultures Ⅱ.Results and Discussion

The estimated kinetic parameters showed that Methanothrix, Methanobacter, Desulfobacter postagei and Desulfovibrio like MPB and SRB were presented during the glucose degradation process. The SRB cannot out-compete MPB for acetate at low concentration of sulfate. This was due to the acetate-utilizing SRB cannot out-compete the hydrogen-utilizing SRB for sulfate, because the latter has a higher (Ks = 10.7 - 13.3 mg/L) affinity than former (19.5 - 21.4 mg/L) for sulfate. It reveals that the hydrogen-utilizing SRB is obviously favorable for acetate-utilizing MPB to out-grew the acetate-utilizing SRB and have been believed to play a critical role in a practical methanogenic process.