Determination of rare earth elements in plant protoplasts by MAA |
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Authors: | Zhiyong Zhang Yuqi Wang Jingxin Sun Fuliang Li Zhifang Chai Lei Xu Xin Li Guoyin Cao |
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Institution: | (1) Institute of High Energy Physics and Laboratory of Nuclear Analytical Techniques, Chinese Academy of Sciences, 100080 Beijing, China;(2) Graduate School of the Chinese Academy of Agriculture, 100081 Beijing, China |
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Abstract: | A preliminary study on the speciation of rare earth elements in plant cells has been carried out by molecular activation analysis
(MAA). Mesophyll protoplasts ofBrassica napus were isolated by enzymatic digestion. After being washed with isosmotic solution containing EDTA for several times, the protoplasts
were purified by gradient centrifugation. Then the concentration of rare earth elements (REEs) in the protoplasts was determined
by neutron activation analysis. The result shows that REEs can enter the cells of the plant. |
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Keywords: | protoplast molecular activation analysis rare earth elements |
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