慢性不可预知性应激刺激建立大鼠颞下颌关节紊乱病模型

目的对大鼠进行人工干预下的情绪应激试验,观察大鼠下颌关节结构的变化,尝试建立颞下颌关节紊乱病(TMD)的动物模型。方法雄性Wistar大鼠16只,随机分为两组,分别为实验组和对照组。实验组进行慢性不可预知性应激试验,对照组正常饲养。观察各组大鼠行为学改变,6周后处死,完整解剖分离下颌髁状突及关节盘,扫描电镜观察。结果实验组大鼠行为学指标改变明显,扫描电镜下显示髁状突表面凝胶不完整,胶原纤维紊乱,断裂,暴露深层胶原组织等,关节盘表面凝胶样物质覆盖仍较完整,但沟回排列不规则,回隆起缩窄降低,沟变浅,对照组无此改变。结论慢性不可预见性应激刺激可造成大鼠下颌关节盘、突退行性改变,符合TMD病理改变,认为建立大鼠颞下颌关节紊乱病动物模型成功。

TMD Animal Model in Rats Induced by Chronic Unpredictable Emotional Stress

Objective To study the influence of emotional stress to the ultrastructures of temporomandibular Disorder in rats,and to establish the TMD animal model.Methods 16 male Wistar rats were dividing into 2 groups randomly,one was of which experimental group,and the other was control group.The experimental group was subjected to make chronic unpredictable stress animal model.After 6 weeks,all of rats were killed and their condyles and articular discs were dissected and taken out completely,observing by scanning electron microscope.Result In experimental group,the ethology of rats changed significantly and under scanning electron microscope,the condyles in experimental group were found to have the surface gelatum cracked,disordered and broken collagen fibrils,and exposed deep layer collagen fibrils.The articular discs' surface gelatum were still integrated,but the gyri dispose were irregular.In control group,there were no such changes in condyles and articular discs.Conclusion The chronic unpredictable stress can induce the ultrastructural injuries of temporomandinbular joint in rats,which is consistent with the pathological changes of TMD,so we presumed it is feasible to induce the animal model of TMD.