Establishment of Genetic Transformation System for Miscanthus sacchariflorus and Obtaining of Its Transgenic Plants |
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| Authors: | Yi Zili Zhou Puhua Chu Chengcai Li Xiang Tian Wenzhong Wang Li Cao Shouyun Tang Zuoshun |
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| Affiliation: | Department of Biotech, Hunan Agricultural University, Changsha 410128, P.R.China;Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, P.R.China |
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| Abstract: | The initiation and regeneration system for embryogenic callus of Miscanthus sacchariflrus is established at very high frequency. Potato proteinaseⅡ(pinⅡ) genes are introduced into the callus of Miscanthus sacchariflorus and some transgenic plants are obtained by the particle bombardment transformation system. PCR and Southern analysis show that target genes are integrated into the genome of these transgenic plants. |
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| Keywords: | Miscanthus sacchariflorus bombardment transformation potato proteinase inhibitor gene |
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