| 脂肪酶催化大豆色拉油甘油解制备二甘酯 |
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| 引用本文： | 张弢,王芳,邓利,聂开立.脂肪酶催化大豆色拉油甘油解制备二甘酯[J].北京化工大学学报(自然科学版),2004,31(5):26-29. |
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| 作者姓名： | 张弢 王芳 邓利 聂开立 |
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| 作者单位： | 北京化工大学生命科学与技术学院,北京,100029;北京化工大学生命科学与技术学院,北京,100029;北京化工大学生命科学与技术学院,北京,100029;北京化工大学生命科学与技术学院,北京,100029 |
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| 基金项目： | 国家高技术研究发展计划(863计划)
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北京市生物加工过程重点实验室基金 |
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| 摘 要： | 研究了脂肪酶催化大豆色拉油甘油解制备二甘酯过程。对多种商业化和自制固定化酶进行了筛选,并对影响甘油解过程的几个重要参数进行了研究,如底物油与甘油摩尔比、酶剂量、初始水质量分数、温度、搅拌方式和溶剂效应。采用递进法进行实验,优化参数的最佳条件为:油与甘油摩尔比1∶1,Novozym435脂肪酶(油质量10%) ,初始水质量分数0% ,反应温度50℃。在无溶剂体系中,反应16h,二甘酯质量分数达到56.3%;当采用叔丁醇作为溶剂,反应24h,二甘酯质量分数达到70.4%。
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| 关 键 词： | 二甘酯 甘油解 脂肪酶催化 |
| 收稿时间： | 2004-01-28 |
| 修稿时间： | 2004年1月8日 |
Prparation of diglyceride by lipase-catalyzed glycerolysis of soybean salad oil with glycerol |
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| Zhang Tao,Wang Fang,Deng Li,Nie Kai-li.Prparation of diglyceride by lipase-catalyzed glycerolysis of soybean salad oil with glycerol[J].Journal of Beijing University of Chemical Technology,2004,31(5):26-29. |
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| Authors: | Zhang Tao Wang Fang Deng Li Nie Kai-li |
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| Institution: | College of Life Science and Technology; Beijing University of Chemical Technology; Beijing 100029; China |
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| Abstract: | Preparation of diglyceride by lipase-catalyzed glycerolysis of soybean salad oil with glycerol in a batch stirred tank reactor was studied. The lipases preparation was chosen based on screening of the lipases from commercial sources as well as that produced in the laboratory. The effect of various parameters including the sutstrate molar ratio, water content, temperature, enzyme dosage and solvent on the glycerolysis catalyzed by Novozym 435 was studied and the 70t% DAG was obtained under the conditions of 10t% lipase dosage,1:1 molar ratio of oil/glycerol,no initial water content at 50℃ after 24h reaction time. |
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| Keywords: | diglyceride glycerolysis lipase-catalyzed |
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