Molecular characterization of the p83/100 proteins of variousBorrelia burgdorferi sensu lato strains |
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| Authors: | D Rößler H Eiffert S Jauris-Heipke G Lehnert G Liegl V Preac-Mursic J Teepe T Schlott E Soutschek B Wilske |
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| Institution: | (1) Max von Pettenkofer-Institut für Hygiene und Medizinische Mikrobiologie, Ludwig-Maximilians-Universität München, Pettenkoferstr. 9a, D-80336 München, Germany;(2) Mikrogen GmbH, Westendstr. 125, D-80339 München, Germany;(3) Hygiene-Institut der Georg-August Universität, Kreuzbergring 57, D-37075 Göttingen, Germany |
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| Abstract: | Conclusions As in OspA-serotyping experiments, theB garinii group (OspA-sterotype 3–7) showed highest diversity within this internal fragment of p83/100, whereas theB. afzelii group (OspA-type 2) and theB. burgdorferi sensu stricto group (OspA-type 1) were nearly identical. Determination of the size of the PCR products as well as restriction fragment length polymorphism analysis (DraI) can be used for classification into the three species ofB. burgdorferi sensu lato. Since p83/100 is chromosomally encoded, this protein might be a more stable marker for classification than the plasmid-encoded OspA. In contrast to the flagellin gene a subclassification of theB. garinii group is possible due to the diversity of the p83/100 internal fragment. |
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