基于N-异丙基丙烯酰胺的温度敏感色谱材料

综述了近年来在基于N-异丙基丙烯酰胺的温度敏感色谱材料的研究进展.从不同的色谱类型出发,综述了影响温度敏色谱材料对分析物分离和富集的因素.仅实现了纯水为流动相下对多肽,氨基酸的良好分离,通过温度控制的捕获和释放方式实现了腺苷核苷酸、蛋白质等生物分子的选择性富集和分离,并成功应用于血清样品的蛋白质组学分析.通过温度的改变可以实现腺苷和辣根过氧化物酶等含有顺式二羟基结构生物分子和凝血酶的选择性富集和分离,并利用温度敏感毛细管柱通过温度的改变对酸类小分子进行了分离.评述了温度敏感色谱材料的研究意义和发展方向,并对其应用前景进行了展望. 

Thermo-Response Chromatography Materials Based on N-Isopropylamide

It has become an important research area to develop new temperature response chromatographic materials. The recent progresses about temperature response chromatographic materials based on poly (N-isopropylacrylamide) (PNIPAAm) were reviewed. And the separation and enrichment factors of analytes on temperature response chromatographic materials were summarized from different types of chromatographic materials. Some achievements were gotten for good separation of peptides and Amino acids under the pure water as the mobile phase, and for the selective enrichment and separation of biomolecules such as adenosine nucleotide and protein by the temperature control. And temperature response material is successfully applied in serum proteomics analysis of samples. Results show that thrombin and the cis-diol containing bimolecular such as adenosine and horseradish peroxides can be enriched and separated with the temperature changing, and the separation of acids molecules using temperature sensitive capillary column by the change of temperature can be realized. Moreover,the research significance, development direction and prospects of its application are commented.