首页 | 本学科首页   官方微博 | 高级检索  
     

海洋细菌11211的生长条件及其几丁质酶研究
引用本文:杨承勇,周世宁,周毅频,林永成. 海洋细菌11211的生长条件及其几丁质酶研究[J]. 中山大学学报(自然科学版), 2000, 39(6): 91-94
作者姓名:杨承勇  周世宁  周毅频  林永成
作者单位:1. 中山大学生命科学学院,广东广州 510275
2. 中国科学院南海海洋研究所,广东广州 510301
3. 中山大学化学与化学工程学院,广东广州 510275
基金项目:国家自然科学基金资助项目(39870023);华南生物科学与技术研究中心资助项目
摘    要:从南海海水中分离到产几丁质酶的弧菌(Vibrio sp.),研究了菌株11211的生长条件和产酶条件。11211菌生长需要Na^+,海水的其他成分对菌生长也有影响。胶体几丁质可诱导几丁质酶产生。在几丁质培养基中11211菌的产酶高峰为生长的第7天。经硫酸铵沉淀,Sephadex G-100、DEAE-纤维素及几丁质柱层析,酶被纯化了18倍。经SDS-PAGE分析,酶的相对分子质量约为30000。该

关 键 词:海洋细菌 几丁质酶 生长条件 11211菌 产酶条件
文章编号:0529-6579(2000)06-0091-04
修稿时间:1999-12-21

Studies on the Growth and Chitinase Production of a Marine Baterium 11211
YANG Cheng yongSchool of Life Sciences,Zhongshan University,Guangzhou ,China,ZHOU Shi ning,ZHOU Yi pin,LIN Yong cheng. Studies on the Growth and Chitinase Production of a Marine Baterium 11211[J]. Acta Scientiarum Naturalium Universitatis Sunyatseni, 2000, 39(6): 91-94
Authors:YANG Cheng yongSchool of Life Sciences  Zhongshan University  Guangzhou   China  ZHOU Shi ning  ZHOU Yi pin  LIN Yong cheng
Affiliation:YANG Cheng yongSchool of Life Sciences,Zhongshan University,Guangzhou 510275,China,ZHOU Shi ning,ZHOU Yi pin,LIN Yong cheng
Abstract:Bacterium 11211 which produces chitinase was isolated from the South China Sea.The growth condition and chitinase production of bacteria were investigated.Na was necessary for bacterium 11211 growth.Colloid chiyin could induce bacteria to produce chitinase.The growth of bacteria was slow in the medium containing colloid chitin as main carbon source,and the enzyme activity reached maximum on 7 d of incubation.Following the purification,the enzyme was characterized and its molecular weight was estimated by SDS PAGE.
Keywords:marine bacteria  chitin  chitinase
本文献已被 CNKI 维普 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号