Cloning and domain structure of the mammalian splicing factor U2AF. |
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| Authors: | P D Zamore J G Patton M R Green |
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| Institution: | Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138. |
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| Abstract: | A complementary DNA clone encoding the large subunit of the essential mammalian pre-messenger RNA splicing component U2 snRNP auxiliary factor (U2AF65) has been isolated and expressed in vitro. It contains two functional domains: a sequence-specific RNA-binding region composed of three ribonucleoprotein-consensus sequence domains, and an arginine/serine-rich motif necessary for splicing but not for binding to pre-mRNA. |
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