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| 不同因子对苜蓿悬浮培养细胞皂苷合成影响的研究 | |
| 引用本文： | 李波,高玉芝,刘国华.不同因子对苜蓿悬浮培养细胞皂苷合成影响的研究[J].高师理科学刊,2009,29(6):60-63. |
| 作者姓名： | 李波 高玉芝 刘国华 |
| 作者单位： | 1. 齐齐哈尔大学,生命科学与工程学院,黑龙江,齐齐哈尔,161006 2. 大庆师范学院,物理与电气信息工程学院,黑龙江,大庆,163319 3. 齐齐哈尔市科协,黑龙江,齐齐哈尔,161006 |
| 基金项目： | 黑龙江省教育厅科学技术研究项目 |
| 摘 要： | 探讨了在苜蓿愈伤组织悬浮细胞培养过程中,培养液初始pH值、活性炭处理量和接种量对苜蓿皂苷积累的影响,采用超声波法提取其中的皂苷并利用紫外分光光度法测定其质量分数.结果表明,初始pH维持在5.6～5.8,2 g/瓶的接种量对皂苷的形成最为有利.但是,随着活性炭用量的增加,苜蓿悬浮培养细胞的皂苷质量分数随之下降. |
| 关 键 词： | 愈伤组织 悬浮培养 pH值 活性炭 接种量 皂苷 |
Research on effect of culture condition factors on synthesis of saponin glycosides in cell suspension of alfalfa |
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| LI Bo,GAO Yu-zhi,LIU Guo-hua.Research on effect of culture condition factors on synthesis of saponin glycosides in cell suspension of alfalfa[J].Journal of Science of Teachers＇College and University,2009,29(6):60-63. | |
| Authors: | LI Bo GAO Yu-zhi LIU Guo-hua |
| Institution: | LI Bo1,GAO Yu-zhi2,LIU Guo-hua3(1.School of Life Science and Engineering,Qiqihar University,Qiqihar 161006,China,2.School of Physics and Electrical Information Engineering,Daqing Teachers College,Daqing 163319,3.Science and Technology Association of Qiqihar,China) |
| Abstract: | Discussed the effects of the initial pH value of culture substance,the treated concentration of active carbon and the weight of inoculation on saponin accumulation in alfalfa callus suspension cell culture process.The ultrasonic method was used to extract the saponin and UV spectrophotometry to determine their contents.The results showed that 30 mL culture substance was loaded in 100 mL flask,maintaining the pH value at the range over 5.6 to 5.8,and the weight of inoculation was 2 g/flask,that is optimum to... |
| Keywords: | callus suspension culture the pH value active carbon the weight of inoculation saponin |
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