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| 鸭胆汁中高纯度分泌型免疫球蛋白A制备 | |
| 引用本文： | 杨晓燕,周霞,汪铭书,齐雪峰,程安春. 鸭胆汁中高纯度分泌型免疫球蛋白A制备[J]. 大理学院学报：综合版, 2009, 8(8): 43-45,58 |
| 作者姓名： | 杨晓燕 周霞 汪铭书 齐雪峰 程安春 |
| 作者单位： | 1. 大理学院生命科学与化学学院,云南大理,671000;四川农业大学动物科技学院禽病防治研究中心,四川雅安,625014 2. 四川农业大学动物科技学院禽病防治研究中心,四川雅安,625014 3. 四川农业大学动物科技学院禽病防治研究中心,四川雅安,625014;西北农林科技大学动物医学院,陕西杨凌,712100 |
| 基金项目： | 教育部新世纪优秀人才支持计划资助项目(NCET-0600818);;高等学校科技创新工程重大项目培育资金资助项目(706050) |
| 摘 要： | 目的:从鸭胆汁中分离纯化分泌型免疫球蛋白A(SIgA)。方法:采用饱和硫酸铵和不同浓度聚乙二醇PEG-6000沉淀法和Micro-Prep S离子交换法从鸭胆汁中提取SIgA。SIgA的纯度和活性经SDS-PAGE和ELISA法检测并进行比较。结果:建立了从鸭胆汁中分离纯化高纯度SIgA的方法。结论:用终浓度15%的PEG-6000沉淀法可从鸭胆汁中获得电泳纯的SIgA,其产量最高,活性最好。 |
| 关 键 词： | SIgA 硫酸铵 聚乙二醇 Macro-Prep S |
Preparation of High Purity Secretory Immunoglobulin A from Duck Bile |
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| Yang Xiaoyan,Zhou Xia,Wang Mingshu,Qi Xuefeng,Cheng Anchun. Preparation of High Purity Secretory Immunoglobulin A from Duck Bile[J]. Journal of Dali University, 2009, 8(8): 43-45,58 | |
| Authors: | Yang Xiaoyan Zhou Xia Wang Mingshu Qi Xuefeng Cheng Anchun |
| Affiliation: | 1.College of Life Science and Chemistry;Dali university;Dali;Yunnan 671000;China;2.Avian Disease Research Center;College of Animal Science and Veterinary Medicine;Sichuan Agricultural University;Ya'an;Sichuan 625014;3.College of Veterinary Medicine;Northwest Agriculture and Forestry University;Yangling;Shanxi 712100;China |
| Abstract: | Objective: To isolate and purify secretory immunoglobulin A(SIgA) from duck bile.Method: Ammonium sulfate precipitation,different concentration of polyethylene glycol(PEG-6000) precipitation and further purification by Macro-Prep S were used to isolate duck SIgA.The Purification and antibody activity of purified SIgA were estimated by SDS-PAGE and ELISA methods,respectively.Result: The method was developed to isolate and purify high purity SIgA from duck bile.Conclusion: The highest output and purity of duc... |
| Keywords: | SIgA ammonium sulfate PEG Macro-Prep S |
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