| 壳聚糖修饰L-天门冬酰胺酶对酶活及抗原的影响 |
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| 引用本文： | 辛秀娟,魏东芝,董常松,周文瑜,刘建文.壳聚糖修饰L-天门冬酰胺酶对酶活及抗原的影响[J].华东理工大学学报(自然科学版),2002,28(4):380-382. |
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| 作者姓名： | 辛秀娟 魏东芝 董常松 周文瑜 刘建文 |
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| 作者单位： | 华东理工大学生物反应器工程国家重点实验室,上海,200237 |
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| 摘 要： | 采用水溶性壳聚糖对L－天门冬酶胺酶进行化学修饰，修饰后的酶活回收率高达705，修饰酶的比活为每毫克蛋白质121.79U，pH值为7.4，更接近于人体血浆pH值(7.2-7.4），对底物的亲和力有所提高，稳定性增加，抗原性仅为自然酶的1／3，增加了临床使用的安全性。
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| 关 键 词： | 酶活 抗原 N 0－羧甲基壳聚糖 L－天门冬酰胺酶 化学修饰 癌细胞 免疫沉淀 生物药品 |
| 文章编号： | 1006-3080(2002)04-0380-03 |
| 修稿时间： | 2001年9月1日 |
Effect of Modification of L-asparaginase by Chitosan on Enzyme Activity a nd Antigenity |
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| XIN Xiu juan,WEI Dong zhi ,DONG Chang song,ZHOU Wen yu,LIU Jian wen.Effect of Modification of L-asparaginase by Chitosan on Enzyme Activity a nd Antigenity[J].Journal of East China University of Science and Technology,2002,28(4):380-382. |
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| Authors: | XIN Xiu juan WEI Dong zhi DONG Chang song ZHOU Wen yu LIU Jian wen |
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| Institution: | XIN Xiu juan,WEI Dong zhi *,DONG Chang song,ZHOU Wen yu,LIU Jian wen |
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| Abstract: | The E.coli L asparaginase was modified by N,O carboxymethyl chitosan. The modified enzyme remained about 70% catalytic activity. Experimental results also showed that the optimal pH of the modified enzyme was about 7.4 and its affinity of L asparagine was stronger than that of the native enzyme. It's stability against trypsin increased and the antigenity was only 1/3 of the native enzyme. It was found that the modified enzyme might be much more useful than that of the native enzyme in clinical treatment of tumor, especially for the acute lymphoblastic leukaemia. |
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| Keywords: | N O carboxymethyl chitosan L asparaginase chemical modification antigen tumor cell immunoprecipitate |
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