| 血清胸腺因子（STF）基因的合成 |
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| 引用本文： | 温晋,陈亚民.血清胸腺因子（STF）基因的合成[J].暨南大学学报,1991,12(3):84-87. |
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| 作者姓名： | 温晋 陈亚民 |
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| 作者单位： | 暨南大学生物学系，暨南大学生物学系，暨南大学生物学系，暨南大学生物学系，暨南大学生物学系，暨南大学生物学系 |
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| 摘 要： |
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| 关 键 词： | 血清胸腺因子 基因 合成 DNA片段 |
CHEMICAL SYNTHESIS OF A GENE FOR SERUM THYMIC FACTOR |
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| Wen Jin Chen Yamin Liang Jianning Zhou Jinxin Liu Feipeng Zhou Tianhong.CHEMICAL SYNTHESIS OF A GENE FOR SERUM THYMIC FACTOR[J].Journal of Jinan University(Natural Science & Medicine Edition),1991,12(3):84-87. |
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| Authors: | Wen Jin Chen Yamin Liang Jianning Zhou Jinxin Liu Feipeng Zhou Tianhong |
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| Institution: | Department of Biology |
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| Abstract: | A chemically synthesized DNA fragment encoding for serum thymic factor (STF) was achieved by using Mqdel 381A DNA synthesizer. The synthetic fragment was designed in such a way that the coding sequence of STF gene is preceeded by ATG for the starting of translation and followed by ATG and TAA for the stopping. The ends of the fragment are BamHI and Sall recogartion Sequence separatly. The synthetic STF gene which cloned in the singlestranded phage M13mp18 was analysed by the method of dedeoxy chaintermination DNA sequencing. The sequence determined demonstrated that the synthetic STF gene was exactly the same as designed. |
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| Keywords: | DNA Synthesis DNA Sequencing Serum thymic factor gene |
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