柱前衍生氨基酸的色谱分析

对氨基酸的反相高效液相色谱的双荧光检测了作了介绍。采用邻苯二甲醛／巯基乙醇与９－芴基氯甲酸酯柱前衍生物，同时采用两台串联的荧光检测器对伯氨基酸和仲氨基酸进行分析；利用高半胱氨酸、高丝氨酸、正缬氨酸（ＮＶＡＬ），硫代脯氨酸（ＴＰＲＯ）四种内标消降了系统误差。同时，由样品各组分保留时间重现性的相对误差及标准氨基酸衍生物的回收率实验来确定该方法的准确性。结果表明：此检测氨基酸方法灵敏度高，检出高且重现性

Pre column Derivatization High Performance Liquid Chrometography for the Analysis of Amino Acids

This paper introduces analysis of RP HPLC for amino acids,which uses OPA/MCE and FMOC derivatives with two fluorescence detectors.The system's error is removed through Internal Standard,i.e.HCYS,HSER,NVAL,TPRO.Meanwhile,the method' accuracy is determined by relative error of recoverity with all kinds of amino acid's ramification.The result shows:it is an ideal method for amino acids'analysis with better sensitivity,detection limits and recoverity,overcoming the limitation by single ramification & only one detector.In addition,it gets over influent of the peak of NH 3 to column.And the relative error of retentive time is <5% & experiment of recycling is >90%,which explains credibility of the method.