| 一种改进的小分子多肽SDS-PAGE方法 |
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| 引用本文： | 徐小华,陈天豹,李珑,倪莉,饶平凡.一种改进的小分子多肽SDS-PAGE方法[J].福州大学学报(自然科学版),2001,29(3):113-115. |
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| 作者姓名： | 徐小华 陈天豹 李珑 倪莉 饶平凡 |
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| 作者单位： | 福州大学生物工程研究所, |
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| 基金项目： | 福建省“2 11”重点学科基金资助项目 !(2 12 15 ) |
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| 摘 要： | 提出了一种改进的SDS -PAGE方法 .采用 0 .46mol/LTris - 0 .0 47mol/Lglycine (pH 9.5 7) + 1.0g/LSDS连续缓冲体系 ,分离胶T =15 % (C =3 % )和浓缩胶T =4% (C=3% ) 时在 3.5～ 6 8.0kD分子量范围内具有良好的线性关系 ,相关系数r =0 .94.该法操作简便分离效果好 ,使用常用的电泳试剂 ,适合于蛋白质酶解产物和一些疾病等的小分子多肽分析
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| 关 键 词： | SDS-PAGE 连续缓冲体系 多肽 |
| 文章编号： | 1000-2243(2001)03-0113-03 |
| 修稿时间： | 2000年11月1日 |
An improved SDS-PAGE method for the separation of low molecular weight polypeptides |
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| XU Xiao-hu,CHEN Tian-bao,LI Long,NI Li,RAO Ping-fan.An improved SDS-PAGE method for the separation of low molecular weight polypeptides[J].Journal of Fuzhou University(Natural Science Edition),2001,29(3):113-115. |
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| Authors: | XU Xiao-hu CHEN Tian-bao LI Long NI Li RAO Ping-fan |
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| Institution: | (Institute of Biotechnology, Fuzhou University, Fuzhou, Fujian 350002, China) |
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| Abstract: | An improved SDS-PAGE method for the separation of low molecular weight polypeptides is studied in this paper. A continuous buffer of 0.46?mol/L Tris-0.047?mol/L glycine (pH?9.57)+1.0?g SDS，is used as buffer for both separation gel and stacking gel as well as electrode buffer in our method. Polypeptides with molecular weights from 3.5 to 68.0?kD can be satisfactorily separated with good linearity (correlation coefficient r=0.94) between the relative mobility and the logarithm of molecular weight. It is a SDS-PAGE method with the advantage of being the most widely used one with greater ease and lower cost， and it is very useful for the study of enzymatic cleavage products and diseases. |
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| Keywords: | SDS-PAGE continuous buffer polypeptides |
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