The effects of decreased glucose concentrations on the in vitro development of the post-blastocyst mouse embryo in a fetal calf serum-or bovine serum albumin-supplemented medium |
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| Authors: | J T Hendryx R J Wordinger |
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| Institution: | (1) Department of Biology, St. Bonaventure University, 14778 St. Bonaventure, New York, USA;(2) Present address: Department of Anatomy, Texas College of Osteopathic Medicine, Camp Bowie at Montgomery, 76107 Fort Worth, Texas, USA |
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| Abstract: | Summary Decreasing the glucose concentration from 1.0 mg/ml to 0.25 mg/ml has no detrimental effects on postblastocyst embryo development when either dialyzed fetal calf serum (20%) or bovine serum albumin (4.0 mg/ml) is used to supplement Eagle's Basal Medium (BME). Development is reduced in both serum-and BSA-supplemented BME devoid of glucose in comparison to glucose controls. Serum-supplemented media support better overall development than BSA-supplemented media. |
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