| 金表面吖啶橙自组装膜制备及其对蛋白质测定 |
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| 引用本文： | 胡文英,傅丽君. 金表面吖啶橙自组装膜制备及其对蛋白质测定[J]. 山西大学学报(自然科学版), 2010, 33(1) |
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| 作者姓名： | 胡文英 傅丽君 |
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| 作者单位： | 莆田学院环境与生命科学系,福建莆田,351100 |
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| 基金项目： | 福建省教育厅科技项目，高校服务海西建设重点项目，莆田市科技计划项目(2009S02 |
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| 摘 要： | 借Au-S的作用,将半胱氨酸组装在金表面上,利用吖啶橙与半胱氨酸之间静电的作用,将荧光试剂吖啶橙间接组装于金表面,构建的AO/L-Cys/Au双层膜具有强的荧光信号.利用吖啶橙与蛋白质间的相互作用,引起膜表面荧光信号灵敏的变化测定蛋白质,对蛋白质检测下限为2.301×10~(-8)g/L.
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| 关 键 词： | 自组装膜 吖啶橙 荧光 蛋白质 |
Preparation of Acridine Orange Self-assembled Multilayers of Gold Base and Interfacial Recognition for Bovine Serum Albumin |
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| HU Wen-ying,FU Li-jun. Preparation of Acridine Orange Self-assembled Multilayers of Gold Base and Interfacial Recognition for Bovine Serum Albumin[J]. Journal of Shanxi University (Natural Science Edit, 2010, 33(1) |
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| Authors: | HU Wen-ying FU Li-jun |
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| Affiliation: | HU Wen-ying,FU Li-jun(Department of Environmental , Life Science,Putian University,Putian 351100,China) |
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| Abstract: | By chemical bonding of Au-S,L-Cysteine(L-Cys) was assembled onto the surface of gold to form a self-assembled bilayer.Fluorescent reagent Acridine Orange (AO) was indirectly assembled onto the gold surface via the electrostatic interaction between Acridine Orange and L-Cysteine (L-Cys) and the AO/L-Cys/Au self-assembled bilayers were constructed.This membrane had strong fluorescence signs.Because of the action between Bovine Serum Albumin and Acridine Orange,the fluorimetrical signs of membrane could be cha... |
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| Keywords: | self-assembled bilayer fluorescence acridine orange bovine serum albumin |
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