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| 沼泽红假单胞菌的分离鉴定及对重金属铜的响应的研究 | |
| 引用本文： | 张娅婷,张继英,陈坤. 沼泽红假单胞菌的分离鉴定及对重金属铜的响应的研究[J]. 河南科学, 2011, 29(11): 1322-1326 |
| 作者姓名： | 张娅婷 张继英 陈坤 |
| 作者单位： | 1. 信阳农业高等专科学校生物技术系,河南信阳464000;信阳师范学院生命科学学院,河南信阳464000 2. 信阳农业高等专科学校生物技术系,河南信阳,464000 3. 信阳师范学院生命科学学院,河南信阳,464000 |
| 基金项目： | 河南省科技厅科技攻关项目(092102310100) |
| 摘 要： | 从自然水体中分离到一株光合细菌并对其进行鉴定.对光合细菌施加不同浓度Cu2+的影响进行了研究.从信阳市南湾水库大坝下层底泥取样,分离纯化到的红色菌株在显微镜下观察,及生化试验后初步鉴定为一株光合细菌.根据16SrRNA的保守序列设计的常用光合细菌引物.PCR扩增DNA片段大小约为400bp,挑选菌液测序.结果在BLAS... |
| 关 键 词： | 重金属 光合细菌 沼泽红假单胞菌 |
Separated and Identified the Rhodopseudanonas Palustris and Studies on the Response of Heavy Metal Cu~(2+) |
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| Zhang Yating,Zhang Jiying,Chen Kun. Separated and Identified the Rhodopseudanonas Palustris and Studies on the Response of Heavy Metal Cu~(2+)[J]. Henan Science, 2011, 29(11): 1322-1326 | |
| Authors: | Zhang Yating Zhang Jiying Chen Kun |
| Affiliation: | Zhang Yating1,2,Zhang Jiying1,Chen Kun2(1.Xinyang Agricultural College,Biological Technology,Xinyang 464000,Henan China,2.College of Life Science,Xinyang Normal University,Henan China) |
| Abstract: | A stain of PSB was separated and identitied.The effects of treatment with Cu2+ of the PSB were analysised in this article.The water sample was gotten from the sediment of the dam of Nanwan reservoir in Xinyang,and taken to pure separate the red strain which is observed under the microscope,after biochemical confirmed to be a PSB.The common used PSB consensus primers were designed according to 16SrRNA consensus sequence.PCR amplification chip was estimated as 400 bp,and we pick the bacteria liquid to sequenc... |
| Keywords: | heavy metal photosynthetic bacterium Rhodopseudanonas palustris |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |