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用统计学方法优化柠檬苦素闪式提取工艺
引用本文:刘静,刘灿,荣永海,荣龙.用统计学方法优化柠檬苦素闪式提取工艺[J].华南理工大学学报(自然科学版),2011,39(9):28-33.
作者姓名:刘静  刘灿  荣永海  荣龙
作者单位:北京航空航天大学生物与医学工程学院,北京,100191
基金项目:广东省教育部产学研结合项目(2009B090300323)
摘    要:以柠檬苦素收率为评价指标,比较了正交实验设计法、均匀设计法和响应曲面法对柠檬苦素闪式提取工艺条件的优化效果.采用最优的闪式提取工艺结合结晶法,从广州新会柑种子中提取分离出柠檬苦素,并通过高效液相色谱检测其纯度.结果表明:获得的柠檬苦素纯度达95%;3种优化方法结果相近,其中响应曲面法得到的最佳提取条件下的柠檬苦素收率最...

关 键 词:柠檬苦素  闪式提取  正交实验设计  均匀设计  响应曲面法

Optimization of Homogenate Extraction Process of Limonin via Statistical Methods
Liu Jing,Liu Can,Rong Yong-hai,Rong Long.Optimization of Homogenate Extraction Process of Limonin via Statistical Methods[J].Journal of South China University of Technology(Natural Science Edition),2011,39(9):28-33.
Authors:Liu Jing  Liu Can  Rong Yong-hai  Rong Long
Institution:Liu Jing Liu Can Rong Yong-hai Rong Long(School of Biological Science and Medical Engineering,Beihang University,Beijing 100191,China)
Abstract:The homogenate extraction of limonin respectively by means of the orthogonal design,the uniform design and the response surface methodology(RSM),with the limonin yield,as the evaluation index.Then,by combining the optimal process with the method of crystallization,limonin was extracted from the seeds of oranges(Citrus reticulata Blanco) in Guangzhou.Moreover,its purity was analyzed through the high-performance liquid chromatography.The results show that the purity of the sample reaches 95%,that the effects ...
Keywords:limonin  homogenate extraction  orthogonal test design  uniform design  response surface methodology  
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