| 枣疯病植原体PCR检测方法的建立及16SrDNA序列分析 |
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| 引用本文： | 张曼,张娅君,李继东,张长征,张典濮. 枣疯病植原体PCR检测方法的建立及16SrDNA序列分析[J]. 河南科学, 2011, 29(9): 1059-1062 |
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| 作者姓名： | 张曼 张娅君 李继东 张长征 张典濮 |
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| 作者单位： | 1. 河南农业大学林学院,郑州,450002
2. 项城市林业局,河南项城,466000 |
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| 基金项目： | 河南省教育厅自然科学计划项目(2010B222002) |
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| 摘 要： | 以新郑灰枣枣疯病叶片为试验材料,先提取枣疯病病株叶片的DNA,经过巢式PCR扩增后对枣疯病植原体16SrDNA进行测序和分析.获得枣疯病植原体的16SrDNA基因片段为1125bp,通过序列同源性比较,表明枣疯病样品中检测到的植原体与EY(AY197655)同源性高达99％,应属于16SrV组,确定了枣疯病植原体的分类...
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| 关 键 词： | 枣疯病 植原体 16SrDNA序列 序列分析 |
The Establishment of PCR Method for Jujube Phytoplasma and the Sequence Analysis of 16SrDNA |
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| Zhang Man,Zhang Yajun,Li Jidong,Zhang Changzheng,Zhang Dianpu. The Establishment of PCR Method for Jujube Phytoplasma and the Sequence Analysis of 16SrDNA[J]. Henan Science, 2011, 29(9): 1059-1062 |
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| Authors: | Zhang Man Zhang Yajun Li Jidong Zhang Changzheng Zhang Dianpu |
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| Affiliation: | Zhang Man1,Zhang Yajun1,Li Jidong1,Zhang Changzheng2,Zhang Dianpu1(1.College of Forestry,Henan Agricultural University,Zhengzhou 450002,China,2.Forestry Bureau of Xiangcheng,Xiangcheng 466000,Henan China) |
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| Abstract: | In this article we choose jujube to Xinzheng ash leaves as experimental material,firstly we extract the DNA from the leaf of infected plant,secondly do the sequencing work of phytoplasma 16SrDNA and then make an analysis after using the method of nested PCR.We can get the result that the phytoplasma of 16SrDNA gene fragment is 1 125bp,through sequence comparison,it shows that the homology of the phytoplasma detected from the jujube plant samples with EY(AY197655) can be up to 99 %,and it must belongs to the... |
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| Keywords: | jujube witches' broom phytoplasma 16SrDNA sequence sequence analysis |
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