真菌Penicillium sp. MO3 产生的抗菌肽AF2的分离纯化和性质的研究

从真菌Penicillium sp. M03发酵液中提取到一种具有抗菌活性的肽类物质AF2，并对其性质进行初步研究. 真菌发酵液经纱布过滤，离心后经10000D超滤膜超滤，冻干，以纯水溶解经Sephadex LH-20，DEAE Sepharose及Sephadex G-15进行分离纯化得到纯净物质，并对其基本性质进行研究. 结果证明：AF2具有典型的肽类物质特征反应，分子量约为1155，含有12种常见氨基酸，对酸碱，胰蛋白酶，β-内酰胺酶稳定，对热有较好的耐受性.

Purification and characterization of a new kind of antimicrobial peptide by fungi-penicillium sp.M03

A kind of antimicrobial peptide AF2 was purified from the broth of fungi. Some properties of AF2 were studied. The broth was subjected to filtration, centrifugation and ultra-filtration. After being freeze-dried, the crude AF2 product was purified by Sephadex LH-20 molecular sieve chromatography , DEAE-Sepharose FF and Sephadex G-15, and a pure component AF2 was obtained. The results show that AF2 has the characteristic reactions of peptides, whose molecular weight is about 1155. The data analysis of amino acids show that AF2 contains twelve kinds of amino acids, Asp, Glu, Ser, Gly, Arg, Thr, Ala, Pro, Cys, Ile, Leu and Phe. AF2 is stable to β-lactamase, acid and alkali, which heat-resistance is also well.